Job ID = 2010308 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 66,104,334 reads read : 132,208,668 reads written : 66,104,334 reads 0-length : 66,104,334 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:11:34 66104334 reads; of these: 66104334 (100.00%) were unpaired; of these: 6922986 (10.47%) aligned 0 times 46857832 (70.88%) aligned exactly 1 time 12323516 (18.64%) aligned >1 times 89.53% overall alignment rate Time searching: 00:11:34 Overall time: 00:11:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 28 files... [bam_rmdupse_core] 40356308 / 59181348 = 0.6819 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:48:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:48:30: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:48:30: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:48:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:48:31: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:48:31: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:48:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:48:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:48:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:48:38: 1000000 INFO @ Fri, 05 Jul 2019 22:48:39: 1000000 INFO @ Fri, 05 Jul 2019 22:48:40: 1000000 INFO @ Fri, 05 Jul 2019 22:48:46: 2000000 INFO @ Fri, 05 Jul 2019 22:48:47: 2000000 INFO @ Fri, 05 Jul 2019 22:48:48: 2000000 INFO @ Fri, 05 Jul 2019 22:48:53: 3000000 INFO @ Fri, 05 Jul 2019 22:48:55: 3000000 INFO @ Fri, 05 Jul 2019 22:48:56: 3000000 INFO @ Fri, 05 Jul 2019 22:49:00: 4000000 INFO @ Fri, 05 Jul 2019 22:49:03: 4000000 INFO @ Fri, 05 Jul 2019 22:49:04: 4000000 INFO @ Fri, 05 Jul 2019 22:49:07: 5000000 INFO @ Fri, 05 Jul 2019 22:49:10: 5000000 INFO @ Fri, 05 Jul 2019 22:49:12: 5000000 INFO @ Fri, 05 Jul 2019 22:49:15: 6000000 INFO @ Fri, 05 Jul 2019 22:49:18: 6000000 INFO @ Fri, 05 Jul 2019 22:49:21: 6000000 INFO @ Fri, 05 Jul 2019 22:49:22: 7000000 INFO @ Fri, 05 Jul 2019 22:49:25: 7000000 INFO @ Fri, 05 Jul 2019 22:49:29: 7000000 INFO @ Fri, 05 Jul 2019 22:49:29: 8000000 INFO @ Fri, 05 Jul 2019 22:49:32: 8000000 INFO @ Fri, 05 Jul 2019 22:49:36: 9000000 INFO @ Fri, 05 Jul 2019 22:49:38: 8000000 INFO @ Fri, 05 Jul 2019 22:49:39: 9000000 INFO @ Fri, 05 Jul 2019 22:49:44: 10000000 INFO @ Fri, 05 Jul 2019 22:49:47: 10000000 INFO @ Fri, 05 Jul 2019 22:49:47: 9000000 INFO @ Fri, 05 Jul 2019 22:49:51: 11000000 INFO @ Fri, 05 Jul 2019 22:49:54: 11000000 INFO @ Fri, 05 Jul 2019 22:49:57: 10000000 INFO @ Fri, 05 Jul 2019 22:49:58: 12000000 INFO @ Fri, 05 Jul 2019 22:50:01: 12000000 INFO @ Fri, 05 Jul 2019 22:50:05: 13000000 INFO @ Fri, 05 Jul 2019 22:50:06: 11000000 INFO @ Fri, 05 Jul 2019 22:50:08: 13000000 INFO @ Fri, 05 Jul 2019 22:50:12: 14000000 INFO @ Fri, 05 Jul 2019 22:50:15: 14000000 INFO @ Fri, 05 Jul 2019 22:50:16: 12000000 INFO @ Fri, 05 Jul 2019 22:50:19: 15000000 INFO @ Fri, 05 Jul 2019 22:50:22: 15000000 INFO @ Fri, 05 Jul 2019 22:50:25: 13000000 INFO @ Fri, 05 Jul 2019 22:50:26: 16000000 INFO @ Fri, 05 Jul 2019 22:50:30: 16000000 INFO @ Fri, 05 Jul 2019 22:50:34: 17000000 INFO @ Fri, 05 Jul 2019 22:50:34: 14000000 INFO @ Fri, 05 Jul 2019 22:50:37: 17000000 INFO @ Fri, 05 Jul 2019 22:50:41: 18000000 INFO @ Fri, 05 Jul 2019 22:50:43: 15000000 INFO @ Fri, 05 Jul 2019 22:50:44: 18000000 INFO @ Fri, 05 Jul 2019 22:50:47: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:50:47: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:50:47: #1 total tags in treatment: 18825040 INFO @ Fri, 05 Jul 2019 22:50:47: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:50:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:50:47: #1 tags after filtering in treatment: 18825040 INFO @ Fri, 05 Jul 2019 22:50:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:50:47: #1 finished! INFO @ Fri, 05 Jul 2019 22:50:47: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:50:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:50:49: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:50:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:50:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:50:50: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:50:50: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:50:50: #1 total tags in treatment: 18825040 INFO @ Fri, 05 Jul 2019 22:50:50: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:50:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:50:51: #1 tags after filtering in treatment: 18825040 INFO @ Fri, 05 Jul 2019 22:50:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:50:51: #1 finished! INFO @ Fri, 05 Jul 2019 22:50:51: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:50:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:50:52: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:50:52: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:50:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:50:53: 16000000 INFO @ Fri, 05 Jul 2019 22:51:03: 17000000 INFO @ Fri, 05 Jul 2019 22:51:12: 18000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 22:51:18: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:51:18: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:51:18: #1 total tags in treatment: 18825040 INFO @ Fri, 05 Jul 2019 22:51:18: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:51:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:51:19: #1 tags after filtering in treatment: 18825040 INFO @ Fri, 05 Jul 2019 22:51:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:51:19: #1 finished! INFO @ Fri, 05 Jul 2019 22:51:19: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:51:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:51:20: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:51:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:51:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3334033/SRX3334033.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。