Job ID = 2010300 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T13:01:49 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - mbedtls_ssl_handshake returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T13:01:49 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - ktls_handshake failed while accessing '130.14.250.26' from '172.19.7.9' 2019-07-05T13:01:49 fasterq-dump.2.9.6 sys: connection failed while opening file within cryptographic module - Failed to create TLS stream for 'sra-download.ncbi.nlm.nih.gov' (130.14.250.26) from '172.19.7.9' 2019-07-05T13:01:49 fasterq-dump.2.9.6 err: connection failed while opening file within cryptographic module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra13/SRR/000926/SRR948406' 2019-07-05T13:01:59 fasterq-dump.2.9.6 err: cmn_iter.c cmn_iter_open_tbl().VDBManagerOpenTableRead( 'SRR948406' ) -> RC(rcDB,rcMgr,rcOpening,rcTable,rcIncorrect) spots read : 2,370,737 reads read : 2,370,737 reads written : 2,370,737 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:17 2370737 reads; of these: 2370737 (100.00%) were unpaired; of these: 1098015 (46.32%) aligned 0 times 1130493 (47.69%) aligned exactly 1 time 142229 (6.00%) aligned >1 times 53.68% overall alignment rate Time searching: 00:00:17 Overall time: 00:00:17 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 499342 / 1272722 = 0.3923 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:04:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:04:36: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:04:36: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:04:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:04:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:04:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:04:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:04:38: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:04:38: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:04:44: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 22:04:44: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 22:04:44: #1 total tags in treatment: 773380 INFO @ Fri, 05 Jul 2019 22:04:44: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:04:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:04:44: #1 tags after filtering in treatment: 773380 INFO @ Fri, 05 Jul 2019 22:04:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:04:44: #1 finished! INFO @ Fri, 05 Jul 2019 22:04:44: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:04:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:04:44: #2 number of paired peaks: 37 WARNING @ Fri, 05 Jul 2019 22:04:44: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:04:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:04:45: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 22:04:45: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 22:04:45: #1 total tags in treatment: 773380 INFO @ Fri, 05 Jul 2019 22:04:45: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:04:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:04:45: #1 tags after filtering in treatment: 773380 INFO @ Fri, 05 Jul 2019 22:04:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:04:45: #1 finished! INFO @ Fri, 05 Jul 2019 22:04:45: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:04:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:04:45: #2 number of paired peaks: 37 WARNING @ Fri, 05 Jul 2019 22:04:45: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:04:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:04:46: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 22:04:46: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 22:04:46: #1 total tags in treatment: 773380 INFO @ Fri, 05 Jul 2019 22:04:46: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:04:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:04:46: #1 tags after filtering in treatment: 773380 INFO @ Fri, 05 Jul 2019 22:04:46: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:04:46: #1 finished! INFO @ Fri, 05 Jul 2019 22:04:46: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:04:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:04:46: #2 number of paired peaks: 37 WARNING @ Fri, 05 Jul 2019 22:04:46: Too few paired peaks (37) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:04:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332106/SRX332106.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。