Job ID = 2010285 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 640,203 reads read : 640,203 reads written : 640,203 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:07 640203 reads; of these: 640203 (100.00%) were unpaired; of these: 219192 (34.24%) aligned 0 times 352061 (54.99%) aligned exactly 1 time 68950 (10.77%) aligned >1 times 65.76% overall alignment rate Time searching: 00:00:07 Overall time: 00:00:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 65227 / 421011 = 0.1549 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:00:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:00:24: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:00:24: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:00:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:00:25: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:00:25: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:00:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:00:26: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:00:26: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:00:27: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 22:00:27: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 22:00:27: #1 total tags in treatment: 355784 INFO @ Fri, 05 Jul 2019 22:00:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:00:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:00:27: #1 tags after filtering in treatment: 355784 INFO @ Fri, 05 Jul 2019 22:00:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:00:27: #1 finished! INFO @ Fri, 05 Jul 2019 22:00:27: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:00:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:00:27: #2 number of paired peaks: 35 WARNING @ Fri, 05 Jul 2019 22:00:27: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:00:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:00:28: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 22:00:28: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 22:00:28: #1 total tags in treatment: 355784 INFO @ Fri, 05 Jul 2019 22:00:28: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:00:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:00:28: #1 tags after filtering in treatment: 355784 INFO @ Fri, 05 Jul 2019 22:00:28: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:00:28: #1 finished! INFO @ Fri, 05 Jul 2019 22:00:28: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:00:28: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:00:28: #2 number of paired peaks: 35 WARNING @ Fri, 05 Jul 2019 22:00:28: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:00:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:00:29: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 22:00:29: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 22:00:29: #1 total tags in treatment: 355784 INFO @ Fri, 05 Jul 2019 22:00:29: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:00:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:00:29: #1 tags after filtering in treatment: 355784 INFO @ Fri, 05 Jul 2019 22:00:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:00:29: #1 finished! INFO @ Fri, 05 Jul 2019 22:00:29: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:00:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:00:30: #2 number of paired peaks: 35 WARNING @ Fri, 05 Jul 2019 22:00:30: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:00:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332086/SRX332086.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。