Job ID = 2010280 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 59,792,319 reads read : 59,792,319 reads written : 59,792,319 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:01 59792319 reads; of these: 59792319 (100.00%) were unpaired; of these: 19247863 (32.19%) aligned 0 times 36499795 (61.04%) aligned exactly 1 time 4044661 (6.76%) aligned >1 times 67.81% overall alignment rate Time searching: 00:06:01 Overall time: 00:06:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 33013522 / 40544456 = 0.8143 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:40:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:40:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:40:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:40:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:40:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:40:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:40:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:40:35: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:40:35: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:40:41: 1000000 INFO @ Fri, 05 Jul 2019 22:40:42: 1000000 INFO @ Fri, 05 Jul 2019 22:40:42: 1000000 INFO @ Fri, 05 Jul 2019 22:40:49: 2000000 INFO @ Fri, 05 Jul 2019 22:40:50: 2000000 INFO @ Fri, 05 Jul 2019 22:40:50: 2000000 INFO @ Fri, 05 Jul 2019 22:40:56: 3000000 INFO @ Fri, 05 Jul 2019 22:40:59: 3000000 INFO @ Fri, 05 Jul 2019 22:40:59: 3000000 INFO @ Fri, 05 Jul 2019 22:41:02: 4000000 INFO @ Fri, 05 Jul 2019 22:41:07: 4000000 INFO @ Fri, 05 Jul 2019 22:41:07: 4000000 INFO @ Fri, 05 Jul 2019 22:41:08: 5000000 INFO @ Fri, 05 Jul 2019 22:41:15: 6000000 INFO @ Fri, 05 Jul 2019 22:41:15: 5000000 INFO @ Fri, 05 Jul 2019 22:41:15: 5000000 INFO @ Fri, 05 Jul 2019 22:41:21: 7000000 INFO @ Fri, 05 Jul 2019 22:41:23: 6000000 INFO @ Fri, 05 Jul 2019 22:41:24: 6000000 INFO @ Fri, 05 Jul 2019 22:41:25: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 22:41:25: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 22:41:25: #1 total tags in treatment: 7530934 INFO @ Fri, 05 Jul 2019 22:41:25: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:41:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:41:25: #1 tags after filtering in treatment: 7530934 INFO @ Fri, 05 Jul 2019 22:41:25: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:41:25: #1 finished! INFO @ Fri, 05 Jul 2019 22:41:25: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:41:25: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:41:25: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:41:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:41:25: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 22:41:30: 7000000 INFO @ Fri, 05 Jul 2019 22:41:31: 7000000 INFO @ Fri, 05 Jul 2019 22:41:34: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 22:41:34: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 22:41:34: #1 total tags in treatment: 7530934 INFO @ Fri, 05 Jul 2019 22:41:34: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:41:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:41:35: #1 tags after filtering in treatment: 7530934 INFO @ Fri, 05 Jul 2019 22:41:35: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:41:35: #1 finished! INFO @ Fri, 05 Jul 2019 22:41:35: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:41:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:41:35: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:41:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:41:35: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 22:41:35: #1 tag size is determined as 40 bps INFO @ Fri, 05 Jul 2019 22:41:35: #1 tag size = 40 INFO @ Fri, 05 Jul 2019 22:41:35: #1 total tags in treatment: 7530934 INFO @ Fri, 05 Jul 2019 22:41:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:41:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:41:36: #1 tags after filtering in treatment: 7530934 INFO @ Fri, 05 Jul 2019 22:41:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:41:36: #1 finished! INFO @ Fri, 05 Jul 2019 22:41:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:41:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:41:36: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:41:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:41:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.20_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.10_peaks.narrowPeak: No such file or directory cut: /home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.05_model.r’rm: : No such file or directorycannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.10_*.xls’ : No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.10_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.20_peaks.narrowPeak’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX332075/SRX332075.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling CompletedMACS2peakCalling CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。