Job ID = 2010266 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 11,680,726 reads read : 23,361,452 reads written : 11,680,726 reads 0-length : 11,680,726 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:57 11680726 reads; of these: 11680726 (100.00%) were unpaired; of these: 727740 (6.23%) aligned 0 times 9441146 (80.83%) aligned exactly 1 time 1511840 (12.94%) aligned >1 times 93.77% overall alignment rate Time searching: 00:02:57 Overall time: 00:02:57 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3199516 / 10952986 = 0.2921 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:07:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:07:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:07:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:07:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:07:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:07:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:07:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:07:18: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:07:18: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:07:23: 1000000 INFO @ Fri, 05 Jul 2019 22:07:25: 1000000 INFO @ Fri, 05 Jul 2019 22:07:27: 1000000 INFO @ Fri, 05 Jul 2019 22:07:30: 2000000 INFO @ Fri, 05 Jul 2019 22:07:33: 2000000 INFO @ Fri, 05 Jul 2019 22:07:36: 2000000 INFO @ Fri, 05 Jul 2019 22:07:37: 3000000 INFO @ Fri, 05 Jul 2019 22:07:42: 3000000 INFO @ Fri, 05 Jul 2019 22:07:43: 4000000 INFO @ Fri, 05 Jul 2019 22:07:45: 3000000 INFO @ Fri, 05 Jul 2019 22:07:50: 5000000 INFO @ Fri, 05 Jul 2019 22:07:50: 4000000 INFO @ Fri, 05 Jul 2019 22:07:53: 4000000 INFO @ Fri, 05 Jul 2019 22:07:56: 6000000 INFO @ Fri, 05 Jul 2019 22:07:58: 5000000 INFO @ Fri, 05 Jul 2019 22:08:01: 5000000 INFO @ Fri, 05 Jul 2019 22:08:03: 7000000 INFO @ Fri, 05 Jul 2019 22:08:06: 6000000 INFO @ Fri, 05 Jul 2019 22:08:08: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:08:08: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:08:08: #1 total tags in treatment: 7753470 INFO @ Fri, 05 Jul 2019 22:08:08: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:08:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:08:08: #1 tags after filtering in treatment: 7753470 INFO @ Fri, 05 Jul 2019 22:08:08: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:08:08: #1 finished! INFO @ Fri, 05 Jul 2019 22:08:08: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:08:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:08:09: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:08:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:08:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:08:09: 6000000 INFO @ Fri, 05 Jul 2019 22:08:14: 7000000 INFO @ Fri, 05 Jul 2019 22:08:17: 7000000 INFO @ Fri, 05 Jul 2019 22:08:20: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:08:20: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:08:20: #1 total tags in treatment: 7753470 INFO @ Fri, 05 Jul 2019 22:08:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:08:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:08:20: #1 tags after filtering in treatment: 7753470 INFO @ Fri, 05 Jul 2019 22:08:20: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:08:20: #1 finished! INFO @ Fri, 05 Jul 2019 22:08:20: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:08:20: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:08:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:08:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:08:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:08:23: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:08:23: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:08:23: #1 total tags in treatment: 7753470 INFO @ Fri, 05 Jul 2019 22:08:23: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:08:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:08:23: #1 tags after filtering in treatment: 7753470 INFO @ Fri, 05 Jul 2019 22:08:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:08:23: #1 finished! INFO @ Fri, 05 Jul 2019 22:08:23: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:08:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:08:24: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:08:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:08:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3316855/SRX3316855.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。