Job ID = 2010264 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,552,192 reads read : 13,104,384 reads written : 6,552,192 reads 0-length : 6,552,192 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:15 6552192 reads; of these: 6552192 (100.00%) were unpaired; of these: 688900 (10.51%) aligned 0 times 4284587 (65.39%) aligned exactly 1 time 1578705 (24.09%) aligned >1 times 89.49% overall alignment rate Time searching: 00:01:15 Overall time: 00:01:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1905776 / 5863292 = 0.3250 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:03:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:03:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:03:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:03:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:03:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:03:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:03:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:03:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:03:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:03:25: 1000000 INFO @ Fri, 05 Jul 2019 22:03:26: 1000000 INFO @ Fri, 05 Jul 2019 22:03:27: 1000000 INFO @ Fri, 05 Jul 2019 22:03:34: 2000000 INFO @ Fri, 05 Jul 2019 22:03:36: 2000000 INFO @ Fri, 05 Jul 2019 22:03:37: 2000000 INFO @ Fri, 05 Jul 2019 22:03:42: 3000000 INFO @ Fri, 05 Jul 2019 22:03:47: 3000000 INFO @ Fri, 05 Jul 2019 22:03:47: 3000000 INFO @ Fri, 05 Jul 2019 22:03:50: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:03:50: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:03:50: #1 total tags in treatment: 3957516 INFO @ Fri, 05 Jul 2019 22:03:50: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:03:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:03:50: #1 tags after filtering in treatment: 3957516 INFO @ Fri, 05 Jul 2019 22:03:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:03:50: #1 finished! INFO @ Fri, 05 Jul 2019 22:03:50: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:03:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:03:50: #2 number of paired peaks: 28 WARNING @ Fri, 05 Jul 2019 22:03:50: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:03:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 532 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:03:57: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:03:57: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:03:57: #1 total tags in treatment: 3957516 INFO @ Fri, 05 Jul 2019 22:03:57: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:03:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:03:57: #1 tags after filtering in treatment: 3957516 INFO @ Fri, 05 Jul 2019 22:03:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:03:57: #1 finished! INFO @ Fri, 05 Jul 2019 22:03:57: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:03:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:03:57: #2 number of paired peaks: 28 WARNING @ Fri, 05 Jul 2019 22:03:57: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:03:57: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.20_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 22:03:57: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:03:57: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:03:57: #1 total tags in treatment: 3957516 INFO @ Fri, 05 Jul 2019 22:03:57: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:03:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:03:57: #1 tags after filtering in treatment: 3957516 INFO @ Fri, 05 Jul 2019 22:03:57: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:03:57: #1 finished! INFO @ Fri, 05 Jul 2019 22:03:57: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:03:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:03:58: #2 number of paired peaks: 28 WARNING @ Fri, 05 Jul 2019 22:03:58: Too few paired peaks (28) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:03:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 314 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294619/SRX3294619.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。