Job ID = 2010246 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 8,570,321 reads read : 17,140,642 reads written : 8,570,321 reads 0-length : 8,570,321 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:25 8570321 reads; of these: 8570321 (100.00%) were unpaired; of these: 1033833 (12.06%) aligned 0 times 6657854 (77.69%) aligned exactly 1 time 878634 (10.25%) aligned >1 times 87.94% overall alignment rate Time searching: 00:01:25 Overall time: 00:01:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1894462 / 7536488 = 0.2514 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:03:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:03:37: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:03:37: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:03:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:03:38: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:03:38: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:03:39: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:03:39: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:03:39: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:03:45: 1000000 INFO @ Fri, 05 Jul 2019 22:03:46: 1000000 INFO @ Fri, 05 Jul 2019 22:03:47: 1000000 INFO @ Fri, 05 Jul 2019 22:03:52: 2000000 INFO @ Fri, 05 Jul 2019 22:03:53: 2000000 INFO @ Fri, 05 Jul 2019 22:03:54: 2000000 INFO @ Fri, 05 Jul 2019 22:03:59: 3000000 INFO @ Fri, 05 Jul 2019 22:04:00: 3000000 INFO @ Fri, 05 Jul 2019 22:04:02: 3000000 INFO @ Fri, 05 Jul 2019 22:04:07: 4000000 INFO @ Fri, 05 Jul 2019 22:04:08: 4000000 INFO @ Fri, 05 Jul 2019 22:04:09: 4000000 INFO @ Fri, 05 Jul 2019 22:04:14: 5000000 INFO @ Fri, 05 Jul 2019 22:04:15: 5000000 INFO @ Fri, 05 Jul 2019 22:04:16: 5000000 INFO @ Fri, 05 Jul 2019 22:04:18: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:04:18: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:04:18: #1 total tags in treatment: 5642026 INFO @ Fri, 05 Jul 2019 22:04:18: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:04:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:04:18: #1 tags after filtering in treatment: 5642026 INFO @ Fri, 05 Jul 2019 22:04:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:04:18: #1 finished! INFO @ Fri, 05 Jul 2019 22:04:18: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:04:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:04:19: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:04:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:04:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:04:19: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:04:19: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:04:19: #1 total tags in treatment: 5642026 INFO @ Fri, 05 Jul 2019 22:04:19: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:04:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:04:19: #1 tags after filtering in treatment: 5642026 INFO @ Fri, 05 Jul 2019 22:04:19: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:04:19: #1 finished! INFO @ Fri, 05 Jul 2019 22:04:19: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:04:19: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:04:20: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:04:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:04:20: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:04:20: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:04:20: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:04:20: #1 total tags in treatment: 5642026 INFO @ Fri, 05 Jul 2019 22:04:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:04:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:04:21: #1 tags after filtering in treatment: 5642026 INFO @ Fri, 05 Jul 2019 22:04:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:04:21: #1 finished! INFO @ Fri, 05 Jul 2019 22:04:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:04:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:04:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:04:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:04:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294614/SRX3294614.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。