Job ID = 2010245 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,009,654 reads read : 18,019,308 reads written : 9,009,654 reads 0-length : 9,009,654 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:54 9009654 reads; of these: 9009654 (100.00%) were unpaired; of these: 795795 (8.83%) aligned 0 times 7211859 (80.05%) aligned exactly 1 time 1002000 (11.12%) aligned >1 times 91.17% overall alignment rate Time searching: 00:01:54 Overall time: 00:01:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3427363 / 8213859 = 0.4173 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:03:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:03:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:03:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:03:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:03:34: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:03:34: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:03:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:03:35: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:03:35: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:03:41: 1000000 INFO @ Fri, 05 Jul 2019 22:03:44: 1000000 INFO @ Fri, 05 Jul 2019 22:03:44: 1000000 INFO @ Fri, 05 Jul 2019 22:03:49: 2000000 INFO @ Fri, 05 Jul 2019 22:03:52: 2000000 INFO @ Fri, 05 Jul 2019 22:03:54: 2000000 INFO @ Fri, 05 Jul 2019 22:03:57: 3000000 INFO @ Fri, 05 Jul 2019 22:04:01: 3000000 INFO @ Fri, 05 Jul 2019 22:04:04: 3000000 INFO @ Fri, 05 Jul 2019 22:04:05: 4000000 INFO @ Fri, 05 Jul 2019 22:04:09: 4000000 INFO @ Fri, 05 Jul 2019 22:04:11: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:04:11: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:04:11: #1 total tags in treatment: 4786496 INFO @ Fri, 05 Jul 2019 22:04:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:04:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:04:11: #1 tags after filtering in treatment: 4786496 INFO @ Fri, 05 Jul 2019 22:04:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:04:11: #1 finished! INFO @ Fri, 05 Jul 2019 22:04:11: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:04:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:04:12: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:04:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:04:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:04:14: 4000000 INFO @ Fri, 05 Jul 2019 22:04:15: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:04:15: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:04:15: #1 total tags in treatment: 4786496 INFO @ Fri, 05 Jul 2019 22:04:15: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:04:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:04:15: #1 tags after filtering in treatment: 4786496 INFO @ Fri, 05 Jul 2019 22:04:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:04:15: #1 finished! INFO @ Fri, 05 Jul 2019 22:04:15: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:04:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:04:16: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:04:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:04:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:04:21: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:04:21: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:04:21: #1 total tags in treatment: 4786496 INFO @ Fri, 05 Jul 2019 22:04:21: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:04:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:04:21: #1 tags after filtering in treatment: 4786496 INFO @ Fri, 05 Jul 2019 22:04:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:04:21: #1 finished! INFO @ Fri, 05 Jul 2019 22:04:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:04:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:04:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:04:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:04:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294613/SRX3294613.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。