Job ID = 2010240 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 7,106,533 reads read : 14,213,066 reads written : 7,106,533 reads 0-length : 7,106,533 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:23 7106533 reads; of these: 7106533 (100.00%) were unpaired; of these: 753918 (10.61%) aligned 0 times 5652655 (79.54%) aligned exactly 1 time 699960 (9.85%) aligned >1 times 89.39% overall alignment rate Time searching: 00:01:23 Overall time: 00:01:23 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1572201 / 6352615 = 0.2475 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:00:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:00:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:00:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:00:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:00:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:00:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:00:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:00:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:00:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:00:22: 1000000 INFO @ Fri, 05 Jul 2019 22:00:24: 1000000 INFO @ Fri, 05 Jul 2019 22:00:25: 1000000 INFO @ Fri, 05 Jul 2019 22:00:29: 2000000 INFO @ Fri, 05 Jul 2019 22:00:32: 2000000 INFO @ Fri, 05 Jul 2019 22:00:32: 2000000 INFO @ Fri, 05 Jul 2019 22:00:36: 3000000 INFO @ Fri, 05 Jul 2019 22:00:40: 3000000 INFO @ Fri, 05 Jul 2019 22:00:40: 3000000 INFO @ Fri, 05 Jul 2019 22:00:42: 4000000 INFO @ Fri, 05 Jul 2019 22:00:47: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:00:47: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:00:47: #1 total tags in treatment: 4780414 INFO @ Fri, 05 Jul 2019 22:00:47: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:00:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:00:47: 4000000 INFO @ Fri, 05 Jul 2019 22:00:47: #1 tags after filtering in treatment: 4780414 INFO @ Fri, 05 Jul 2019 22:00:47: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:00:47: #1 finished! INFO @ Fri, 05 Jul 2019 22:00:47: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:00:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:00:48: 4000000 INFO @ Fri, 05 Jul 2019 22:00:48: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:00:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:00:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:00:53: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:00:53: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:00:53: #1 total tags in treatment: 4780414 INFO @ Fri, 05 Jul 2019 22:00:53: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:00:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:00:53: #1 tags after filtering in treatment: 4780414 INFO @ Fri, 05 Jul 2019 22:00:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:00:53: #1 finished! INFO @ Fri, 05 Jul 2019 22:00:53: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:00:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:00:53: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:00:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:00:53: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 22:00:53: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:00:53: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:00:53: #1 total tags in treatment: 4780414 INFO @ Fri, 05 Jul 2019 22:00:53: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:00:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:00:53: #1 tags after filtering in treatment: 4780414 INFO @ Fri, 05 Jul 2019 22:00:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:00:53: #1 finished! INFO @ Fri, 05 Jul 2019 22:00:53: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:00:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:00:54: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:00:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:00:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294608/SRX3294608.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。