Job ID = 2010233 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,042,357 reads read : 24,084,714 reads written : 12,042,357 reads 0-length : 12,042,357 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:14 12042357 reads; of these: 12042357 (100.00%) were unpaired; of these: 926565 (7.69%) aligned 0 times 9695450 (80.51%) aligned exactly 1 time 1420342 (11.79%) aligned >1 times 92.31% overall alignment rate Time searching: 00:02:14 Overall time: 00:02:14 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3438399 / 11115792 = 0.3093 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:01:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:01:42: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:01:42: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:01:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:01:43: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:01:43: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:01:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:01:44: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:01:44: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:01:53: 1000000 INFO @ Fri, 05 Jul 2019 22:01:54: 1000000 INFO @ Fri, 05 Jul 2019 22:01:56: 1000000 INFO @ Fri, 05 Jul 2019 22:02:01: 2000000 INFO @ Fri, 05 Jul 2019 22:02:06: 2000000 INFO @ Fri, 05 Jul 2019 22:02:08: 2000000 INFO @ Fri, 05 Jul 2019 22:02:10: 3000000 INFO @ Fri, 05 Jul 2019 22:02:18: 4000000 INFO @ Fri, 05 Jul 2019 22:02:18: 3000000 INFO @ Fri, 05 Jul 2019 22:02:20: 3000000 INFO @ Fri, 05 Jul 2019 22:02:26: 5000000 INFO @ Fri, 05 Jul 2019 22:02:29: 4000000 INFO @ Fri, 05 Jul 2019 22:02:31: 4000000 INFO @ Fri, 05 Jul 2019 22:02:35: 6000000 INFO @ Fri, 05 Jul 2019 22:02:40: 5000000 INFO @ Fri, 05 Jul 2019 22:02:42: 5000000 INFO @ Fri, 05 Jul 2019 22:02:44: 7000000 INFO @ Fri, 05 Jul 2019 22:02:50: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:02:50: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:02:50: #1 total tags in treatment: 7677393 INFO @ Fri, 05 Jul 2019 22:02:50: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:02:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:02:50: #1 tags after filtering in treatment: 7677393 INFO @ Fri, 05 Jul 2019 22:02:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:02:50: #1 finished! INFO @ Fri, 05 Jul 2019 22:02:50: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:02:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:02:50: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:02:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:02:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:02:51: 6000000 INFO @ Fri, 05 Jul 2019 22:02:53: 6000000 INFO @ Fri, 05 Jul 2019 22:03:02: 7000000 INFO @ Fri, 05 Jul 2019 22:03:04: 7000000 INFO @ Fri, 05 Jul 2019 22:03:09: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:03:09: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:03:09: #1 total tags in treatment: 7677393 INFO @ Fri, 05 Jul 2019 22:03:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:03:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:03:09: #1 tags after filtering in treatment: 7677393 INFO @ Fri, 05 Jul 2019 22:03:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:03:09: #1 finished! INFO @ Fri, 05 Jul 2019 22:03:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:03:09: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 05 Jul 2019 22:03:10: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:03:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:03:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:03:11: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 22:03:11: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 22:03:11: #1 total tags in treatment: 7677393 INFO @ Fri, 05 Jul 2019 22:03:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:03:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:03:11: #1 tags after filtering in treatment: 7677393 INFO @ Fri, 05 Jul 2019 22:03:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:03:11: #1 finished! INFO @ Fri, 05 Jul 2019 22:03:11: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:03:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:03:12: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:03:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:03:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294603/SRX3294603.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。