Job ID = 2010229 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 6,992,136 reads read : 13,984,272 reads written : 6,992,136 reads 0-length : 6,992,136 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:18 6992136 reads; of these: 6992136 (100.00%) were unpaired; of these: 524677 (7.50%) aligned 0 times 5570876 (79.67%) aligned exactly 1 time 896583 (12.82%) aligned >1 times 92.50% overall alignment rate Time searching: 00:01:18 Overall time: 00:01:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1517035 / 6467459 = 0.2346 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:57:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:57:22: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:57:22: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:57:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:57:23: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:57:23: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:57:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:57:24: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:57:24: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:57:30: 1000000 INFO @ Fri, 05 Jul 2019 21:57:32: 1000000 INFO @ Fri, 05 Jul 2019 21:57:33: 1000000 INFO @ Fri, 05 Jul 2019 21:57:39: 2000000 INFO @ Fri, 05 Jul 2019 21:57:39: 2000000 INFO @ Fri, 05 Jul 2019 21:57:42: 2000000 INFO @ Fri, 05 Jul 2019 21:57:46: 3000000 INFO @ Fri, 05 Jul 2019 21:57:47: 3000000 INFO @ Fri, 05 Jul 2019 21:57:51: 3000000 INFO @ Fri, 05 Jul 2019 21:57:54: 4000000 INFO @ Fri, 05 Jul 2019 21:57:55: 4000000 INFO @ Fri, 05 Jul 2019 21:58:00: 4000000 INFO @ Fri, 05 Jul 2019 21:58:01: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:58:01: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:58:01: #1 total tags in treatment: 4950424 INFO @ Fri, 05 Jul 2019 21:58:01: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:58:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:58:01: #1 tags after filtering in treatment: 4950424 INFO @ Fri, 05 Jul 2019 21:58:01: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:58:01: #1 finished! INFO @ Fri, 05 Jul 2019 21:58:01: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:58:01: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:58:01: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:58:01: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:58:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:58:02: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:58:02: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:58:02: #1 total tags in treatment: 4950424 INFO @ Fri, 05 Jul 2019 21:58:02: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:58:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:58:03: #1 tags after filtering in treatment: 4950424 INFO @ Fri, 05 Jul 2019 21:58:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:58:03: #1 finished! INFO @ Fri, 05 Jul 2019 21:58:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:58:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:58:03: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:58:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:58:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:58:09: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:58:09: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:58:09: #1 total tags in treatment: 4950424 INFO @ Fri, 05 Jul 2019 21:58:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:58:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:58:09: #1 tags after filtering in treatment: 4950424 INFO @ Fri, 05 Jul 2019 21:58:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:58:09: #1 finished! INFO @ Fri, 05 Jul 2019 21:58:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:58:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:58:09: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:58:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:58:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294599/SRX3294599.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。