Job ID = 2010220 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T12:48:20 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T12:48:20 fasterq-dump.2.9.6 err: connection busy while validating within network system module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra53/SRR/006039/SRR6184442' 2019-07-05T12:48:20 fasterq-dump.2.9.6 err: invalid accession 'SRR6184442' 2019-07-05T12:48:35 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T12:48:35 fasterq-dump.2.9.6 err: connection busy while validating within network system module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra53/SRR/006039/SRR6184442' 2019-07-05T12:48:35 fasterq-dump.2.9.6 err: invalid accession 'SRR6184442' 2019-07-05T12:48:50 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T12:48:50 fasterq-dump.2.9.6 err: connection busy while validating within network system module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra53/SRR/006039/SRR6184442' 2019-07-05T12:48:50 fasterq-dump.2.9.6 err: invalid accession 'SRR6184442' 2019-07-05T12:49:05 fasterq-dump.2.9.6 sys: connection busy while validating within network system module - Failed to Make Connection in KClientHttpOpen to 'sra-download.ncbi.nlm.nih.gov:443' 2019-07-05T12:49:05 fasterq-dump.2.9.6 err: connection busy while validating within network system module - error with https open 'https://sra-download.ncbi.nlm.nih.gov/traces/sra53/SRR/006039/SRR6184442' 2019-07-05T12:49:05 fasterq-dump.2.9.6 err: invalid accession 'SRR6184442' spots read : 7,559,310 reads read : 15,118,620 reads written : 7,559,310 reads 0-length : 7,559,310 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:22 7559310 reads; of these: 7559310 (100.00%) were unpaired; of these: 595121 (7.87%) aligned 0 times 6137616 (81.19%) aligned exactly 1 time 826573 (10.93%) aligned >1 times 92.13% overall alignment rate Time searching: 00:01:22 Overall time: 00:01:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1576937 / 6964189 = 0.2264 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:57:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:57:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:57:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:57:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:57:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:57:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:57:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:57:24: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:57:24: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:57:27: 1000000 INFO @ Fri, 05 Jul 2019 21:57:29: 1000000 INFO @ Fri, 05 Jul 2019 21:57:35: 1000000 INFO @ Fri, 05 Jul 2019 21:57:38: 2000000 INFO @ Fri, 05 Jul 2019 21:57:41: 2000000 INFO @ Fri, 05 Jul 2019 21:57:45: 2000000 INFO @ Fri, 05 Jul 2019 21:57:50: 3000000 INFO @ Fri, 05 Jul 2019 21:57:52: 3000000 INFO @ Fri, 05 Jul 2019 21:57:56: 3000000 INFO @ Fri, 05 Jul 2019 21:58:01: 4000000 INFO @ Fri, 05 Jul 2019 21:58:03: 4000000 INFO @ Fri, 05 Jul 2019 21:58:06: 4000000 INFO @ Fri, 05 Jul 2019 21:58:12: 5000000 INFO @ Fri, 05 Jul 2019 21:58:14: 5000000 INFO @ Fri, 05 Jul 2019 21:58:15: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:58:15: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:58:15: #1 total tags in treatment: 5387252 INFO @ Fri, 05 Jul 2019 21:58:15: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:58:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:58:15: #1 tags after filtering in treatment: 5387252 INFO @ Fri, 05 Jul 2019 21:58:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:58:15: #1 finished! INFO @ Fri, 05 Jul 2019 21:58:15: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:58:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:58:15: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:58:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:58:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:58:17: 5000000 INFO @ Fri, 05 Jul 2019 21:58:18: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:58:18: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:58:18: #1 total tags in treatment: 5387252 INFO @ Fri, 05 Jul 2019 21:58:18: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:58:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:58:18: #1 tags after filtering in treatment: 5387252 INFO @ Fri, 05 Jul 2019 21:58:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:58:18: #1 finished! INFO @ Fri, 05 Jul 2019 21:58:18: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:58:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:58:18: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:58:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:58:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:58:21: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:58:21: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:58:21: #1 total tags in treatment: 5387252 INFO @ Fri, 05 Jul 2019 21:58:21: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:58:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:58:21: #1 tags after filtering in treatment: 5387252 INFO @ Fri, 05 Jul 2019 21:58:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:58:21: #1 finished! INFO @ Fri, 05 Jul 2019 21:58:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:58:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:58:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:58:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:58:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294590/SRX3294590.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。