Job ID = 2010207 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 9,525,061 reads read : 19,050,122 reads written : 9,525,061 reads 0-length : 9,525,061 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:04 9525061 reads; of these: 9525061 (100.00%) were unpaired; of these: 557569 (5.85%) aligned 0 times 8225781 (86.36%) aligned exactly 1 time 741711 (7.79%) aligned >1 times 94.15% overall alignment rate Time searching: 00:02:04 Overall time: 00:02:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2853940 / 8967492 = 0.3183 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:51:28: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:51:28: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:51:28: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:51:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:51:29: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:51:29: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:51:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:51:30: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:51:30: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:51:37: 1000000 INFO @ Fri, 05 Jul 2019 21:51:39: 1000000 INFO @ Fri, 05 Jul 2019 21:51:39: 1000000 INFO @ Fri, 05 Jul 2019 21:51:45: 2000000 INFO @ Fri, 05 Jul 2019 21:51:49: 2000000 INFO @ Fri, 05 Jul 2019 21:51:49: 2000000 INFO @ Fri, 05 Jul 2019 21:51:53: 3000000 INFO @ Fri, 05 Jul 2019 21:51:57: 3000000 INFO @ Fri, 05 Jul 2019 21:52:00: 3000000 INFO @ Fri, 05 Jul 2019 21:52:01: 4000000 INFO @ Fri, 05 Jul 2019 21:52:05: 4000000 INFO @ Fri, 05 Jul 2019 21:52:09: 5000000 INFO @ Fri, 05 Jul 2019 21:52:09: 4000000 INFO @ Fri, 05 Jul 2019 21:52:13: 5000000 INFO @ Fri, 05 Jul 2019 21:52:17: 6000000 INFO @ Fri, 05 Jul 2019 21:52:18: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:52:18: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:52:18: #1 total tags in treatment: 6113552 INFO @ Fri, 05 Jul 2019 21:52:18: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:52:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:52:18: #1 tags after filtering in treatment: 6113552 INFO @ Fri, 05 Jul 2019 21:52:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:52:18: #1 finished! INFO @ Fri, 05 Jul 2019 21:52:18: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:52:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:52:19: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:52:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:52:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:52:19: 5000000 INFO @ Fri, 05 Jul 2019 21:52:21: 6000000 INFO @ Fri, 05 Jul 2019 21:52:22: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:52:22: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:52:22: #1 total tags in treatment: 6113552 INFO @ Fri, 05 Jul 2019 21:52:22: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:52:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:52:22: #1 tags after filtering in treatment: 6113552 INFO @ Fri, 05 Jul 2019 21:52:22: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:52:22: #1 finished! INFO @ Fri, 05 Jul 2019 21:52:22: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:52:22: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:52:23: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:52:23: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:52:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:52:28: 6000000 INFO @ Fri, 05 Jul 2019 21:52:29: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:52:29: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:52:29: #1 total tags in treatment: 6113552 INFO @ Fri, 05 Jul 2019 21:52:29: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:52:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:52:29: #1 tags after filtering in treatment: 6113552 INFO @ Fri, 05 Jul 2019 21:52:29: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:52:29: #1 finished! INFO @ Fri, 05 Jul 2019 21:52:29: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:52:29: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:52:30: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:52:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:52:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294579/SRX3294579.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。