Job ID = 2010202 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,820,094 reads read : 41,640,188 reads written : 20,820,094 reads 0-length : 20,820,094 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:12:28 20820094 reads; of these: 20820094 (100.00%) were unpaired; of these: 1422809 (6.83%) aligned 0 times 17221270 (82.71%) aligned exactly 1 time 2176015 (10.45%) aligned >1 times 93.17% overall alignment rate Time searching: 00:12:28 Overall time: 00:12:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7760690 / 19397285 = 0.4001 in library ' ' BAM に変換しました。 Bed ファイルを作成中... INFO @ Fri, 05 Jul 2019 22:06:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off BedGraph に変換中... INFO @ Fri, 05 Jul 2019 22:06:07: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:06:07: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:06:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:06:08: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:06:08: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:06:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 22:06:09: #1 read tag files... INFO @ Fri, 05 Jul 2019 22:06:09: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 22:06:14: 1000000 INFO @ Fri, 05 Jul 2019 22:06:16: 1000000 INFO @ Fri, 05 Jul 2019 22:06:17: 1000000 INFO @ Fri, 05 Jul 2019 22:06:20: 2000000 INFO @ Fri, 05 Jul 2019 22:06:24: 2000000 INFO @ Fri, 05 Jul 2019 22:06:24: 2000000 INFO @ Fri, 05 Jul 2019 22:06:27: 3000000 INFO @ Fri, 05 Jul 2019 22:06:32: 3000000 INFO @ Fri, 05 Jul 2019 22:06:33: 3000000 INFO @ Fri, 05 Jul 2019 22:06:33: 4000000 INFO @ Fri, 05 Jul 2019 22:06:39: 4000000 INFO @ Fri, 05 Jul 2019 22:06:40: 5000000 INFO @ Fri, 05 Jul 2019 22:06:42: 4000000 INFO @ Fri, 05 Jul 2019 22:06:47: 6000000 INFO @ Fri, 05 Jul 2019 22:06:47: 5000000 INFO @ Fri, 05 Jul 2019 22:06:50: 5000000 INFO @ Fri, 05 Jul 2019 22:06:53: 7000000 INFO @ Fri, 05 Jul 2019 22:06:54: 6000000 INFO @ Fri, 05 Jul 2019 22:06:59: 6000000 INFO @ Fri, 05 Jul 2019 22:06:59: 8000000 INFO @ Fri, 05 Jul 2019 22:07:02: 7000000 INFO @ Fri, 05 Jul 2019 22:07:06: 9000000 INFO @ Fri, 05 Jul 2019 22:07:07: 7000000 INFO @ Fri, 05 Jul 2019 22:07:09: 8000000 INFO @ Fri, 05 Jul 2019 22:07:12: 10000000 INFO @ Fri, 05 Jul 2019 22:07:15: 8000000 INFO @ Fri, 05 Jul 2019 22:07:16: 9000000 INFO @ Fri, 05 Jul 2019 22:07:19: 11000000 INFO @ Fri, 05 Jul 2019 22:07:23: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:07:23: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:07:23: #1 total tags in treatment: 11636595 INFO @ Fri, 05 Jul 2019 22:07:23: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:07:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:07:23: #1 tags after filtering in treatment: 11636595 INFO @ Fri, 05 Jul 2019 22:07:23: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:07:23: #1 finished! INFO @ Fri, 05 Jul 2019 22:07:23: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:07:23: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:07:24: 9000000 INFO @ Fri, 05 Jul 2019 22:07:24: 10000000 INFO @ Fri, 05 Jul 2019 22:07:24: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:07:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:07:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:07:31: 11000000 INFO @ Fri, 05 Jul 2019 22:07:32: 10000000 INFO @ Fri, 05 Jul 2019 22:07:36: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:07:36: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:07:36: #1 total tags in treatment: 11636595 INFO @ Fri, 05 Jul 2019 22:07:36: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:07:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:07:36: #1 tags after filtering in treatment: 11636595 INFO @ Fri, 05 Jul 2019 22:07:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:07:36: #1 finished! INFO @ Fri, 05 Jul 2019 22:07:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:07:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:07:37: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:07:37: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:07:37: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 22:07:40: 11000000 INFO @ Fri, 05 Jul 2019 22:07:45: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 22:07:45: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 22:07:45: #1 total tags in treatment: 11636595 INFO @ Fri, 05 Jul 2019 22:07:45: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 22:07:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 22:07:45: #1 tags after filtering in treatment: 11636595 INFO @ Fri, 05 Jul 2019 22:07:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 22:07:45: #1 finished! INFO @ Fri, 05 Jul 2019 22:07:45: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 22:07:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 22:07:46: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 22:07:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 22:07:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294574/SRX3294574.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。