Job ID = 2010200 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T12:41:50 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T12:42:36 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T12:42:36 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 15,867,474 reads read : 31,734,948 reads written : 15,867,474 reads 0-length : 15,867,474 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:03 15867474 reads; of these: 15867474 (100.00%) were unpaired; of these: 916645 (5.78%) aligned 0 times 12609119 (79.47%) aligned exactly 1 time 2341710 (14.76%) aligned >1 times 94.22% overall alignment rate Time searching: 00:03:03 Overall time: 00:03:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7917681 / 14950829 = 0.5296 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:54:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:54:19: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:54:19: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:54:20: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:54:20: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:54:20: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:54:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:54:21: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:54:21: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:54:26: 1000000 INFO @ Fri, 05 Jul 2019 21:54:27: 1000000 INFO @ Fri, 05 Jul 2019 21:54:28: 1000000 INFO @ Fri, 05 Jul 2019 21:54:34: 2000000 INFO @ Fri, 05 Jul 2019 21:54:35: 2000000 INFO @ Fri, 05 Jul 2019 21:54:35: 2000000 INFO @ Fri, 05 Jul 2019 21:54:41: 3000000 INFO @ Fri, 05 Jul 2019 21:54:42: 3000000 INFO @ Fri, 05 Jul 2019 21:54:43: 3000000 INFO @ Fri, 05 Jul 2019 21:54:49: 4000000 INFO @ Fri, 05 Jul 2019 21:54:49: 4000000 INFO @ Fri, 05 Jul 2019 21:54:50: 4000000 INFO @ Fri, 05 Jul 2019 21:54:55: 5000000 INFO @ Fri, 05 Jul 2019 21:54:56: 5000000 INFO @ Fri, 05 Jul 2019 21:54:57: 5000000 INFO @ Fri, 05 Jul 2019 21:55:02: 6000000 INFO @ Fri, 05 Jul 2019 21:55:03: 6000000 INFO @ Fri, 05 Jul 2019 21:55:04: 6000000 INFO @ Fri, 05 Jul 2019 21:55:09: 7000000 INFO @ Fri, 05 Jul 2019 21:55:09: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:55:09: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:55:09: #1 total tags in treatment: 7033148 INFO @ Fri, 05 Jul 2019 21:55:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:55:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:55:09: #1 tags after filtering in treatment: 7033148 INFO @ Fri, 05 Jul 2019 21:55:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:55:09: #1 finished! INFO @ Fri, 05 Jul 2019 21:55:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:55:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:55:10: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:55:10: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:55:10: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:55:10: 7000000 INFO @ Fri, 05 Jul 2019 21:55:11: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:55:11: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:55:11: #1 total tags in treatment: 7033148 INFO @ Fri, 05 Jul 2019 21:55:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:55:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:55:11: #1 tags after filtering in treatment: 7033148 INFO @ Fri, 05 Jul 2019 21:55:11: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:55:11: #1 finished! INFO @ Fri, 05 Jul 2019 21:55:11: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:55:11: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:55:11: 7000000 INFO @ Fri, 05 Jul 2019 21:55:11: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:55:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:55:11: Process for pairing-model is terminated! INFO @ Fri, 05 Jul 2019 21:55:11: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:55:11: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:55:11: #1 total tags in treatment: 7033148 INFO @ Fri, 05 Jul 2019 21:55:11: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:55:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:55:12: #1 tags after filtering in treatment: 7033148 INFO @ Fri, 05 Jul 2019 21:55:12: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:55:12: #1 finished! INFO @ Fri, 05 Jul 2019 21:55:12: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:55:12: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:55:12: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:55:12: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:55:12: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294573/SRX3294573.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。