Job ID = 2010196 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T12:42:59 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T12:43:56 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) 2019-07-05T12:44:42 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 18,970,150 reads read : 37,940,300 reads written : 18,970,150 reads 0-length : 18,970,150 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:26 18970150 reads; of these: 18970150 (100.00%) were unpaired; of these: 960100 (5.06%) aligned 0 times 15130738 (79.76%) aligned exactly 1 time 2879312 (15.18%) aligned >1 times 94.94% overall alignment rate Time searching: 00:03:26 Overall time: 00:03:26 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8990370 / 18010050 = 0.4992 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:57:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:57:14: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:57:14: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:57:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:57:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:57:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:57:22: 1000000 INFO @ Fri, 05 Jul 2019 21:57:22: 1000000 INFO @ Fri, 05 Jul 2019 21:57:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:57:24: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:57:24: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:57:29: 2000000 INFO @ Fri, 05 Jul 2019 21:57:30: 2000000 INFO @ Fri, 05 Jul 2019 21:57:33: 1000000 INFO @ Fri, 05 Jul 2019 21:57:36: 3000000 INFO @ Fri, 05 Jul 2019 21:57:38: 3000000 INFO @ Fri, 05 Jul 2019 21:57:41: 2000000 INFO @ Fri, 05 Jul 2019 21:57:43: 4000000 INFO @ Fri, 05 Jul 2019 21:57:47: 4000000 INFO @ Fri, 05 Jul 2019 21:57:50: 3000000 INFO @ Fri, 05 Jul 2019 21:57:50: 5000000 INFO @ Fri, 05 Jul 2019 21:57:55: 5000000 INFO @ Fri, 05 Jul 2019 21:57:57: 6000000 INFO @ Fri, 05 Jul 2019 21:57:58: 4000000 INFO @ Fri, 05 Jul 2019 21:58:03: 6000000 INFO @ Fri, 05 Jul 2019 21:58:04: 7000000 INFO @ Fri, 05 Jul 2019 21:58:06: 5000000 INFO @ Fri, 05 Jul 2019 21:58:11: 8000000 INFO @ Fri, 05 Jul 2019 21:58:11: 7000000 INFO @ Fri, 05 Jul 2019 21:58:14: 6000000 INFO @ Fri, 05 Jul 2019 21:58:18: 9000000 INFO @ Fri, 05 Jul 2019 21:58:18: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:58:18: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:58:18: #1 total tags in treatment: 9019680 INFO @ Fri, 05 Jul 2019 21:58:18: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:58:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:58:18: #1 tags after filtering in treatment: 9019680 INFO @ Fri, 05 Jul 2019 21:58:18: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:58:18: #1 finished! INFO @ Fri, 05 Jul 2019 21:58:18: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:58:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:58:19: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:58:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:58:19: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:58:19: 8000000 INFO @ Fri, 05 Jul 2019 21:58:22: 7000000 INFO @ Fri, 05 Jul 2019 21:58:26: 9000000 INFO @ Fri, 05 Jul 2019 21:58:27: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:58:27: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:58:27: #1 total tags in treatment: 9019680 INFO @ Fri, 05 Jul 2019 21:58:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:58:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:58:27: #1 tags after filtering in treatment: 9019680 INFO @ Fri, 05 Jul 2019 21:58:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:58:27: #1 finished! INFO @ Fri, 05 Jul 2019 21:58:27: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:58:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:58:27: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:58:27: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:58:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:58:30: 8000000 INFO @ Fri, 05 Jul 2019 21:58:37: 9000000 INFO @ Fri, 05 Jul 2019 21:58:38: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:58:38: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:58:38: #1 total tags in treatment: 9019680 INFO @ Fri, 05 Jul 2019 21:58:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:58:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:58:38: #1 tags after filtering in treatment: 9019680 INFO @ Fri, 05 Jul 2019 21:58:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:58:38: #1 finished! INFO @ Fri, 05 Jul 2019 21:58:38: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:58:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:58:38: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:58:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:58:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294569/SRX3294569.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。