Job ID = 2010195 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,369,597 reads read : 36,739,194 reads written : 18,369,597 reads 0-length : 18,369,597 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:11 18369597 reads; of these: 18369597 (100.00%) were unpaired; of these: 1421694 (7.74%) aligned 0 times 14798740 (80.56%) aligned exactly 1 time 2149163 (11.70%) aligned >1 times 92.26% overall alignment rate Time searching: 00:03:11 Overall time: 00:03:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6418741 / 16947903 = 0.3787 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:54:13: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:54:13: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:54:13: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:54:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:54:14: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:54:14: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:54:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:54:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:54:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:54:22: 1000000 INFO @ Fri, 05 Jul 2019 21:54:23: 1000000 INFO @ Fri, 05 Jul 2019 21:54:24: 1000000 INFO @ Fri, 05 Jul 2019 21:54:31: 2000000 INFO @ Fri, 05 Jul 2019 21:54:32: 2000000 INFO @ Fri, 05 Jul 2019 21:54:33: 2000000 INFO @ Fri, 05 Jul 2019 21:54:40: 3000000 INFO @ Fri, 05 Jul 2019 21:54:40: 3000000 INFO @ Fri, 05 Jul 2019 21:54:42: 3000000 INFO @ Fri, 05 Jul 2019 21:54:48: 4000000 INFO @ Fri, 05 Jul 2019 21:54:49: 4000000 INFO @ Fri, 05 Jul 2019 21:54:52: 4000000 INFO @ Fri, 05 Jul 2019 21:54:56: 5000000 INFO @ Fri, 05 Jul 2019 21:54:58: 5000000 INFO @ Fri, 05 Jul 2019 21:55:01: 5000000 INFO @ Fri, 05 Jul 2019 21:55:04: 6000000 INFO @ Fri, 05 Jul 2019 21:55:07: 6000000 INFO @ Fri, 05 Jul 2019 21:55:10: 6000000 INFO @ Fri, 05 Jul 2019 21:55:12: 7000000 INFO @ Fri, 05 Jul 2019 21:55:16: 7000000 INFO @ Fri, 05 Jul 2019 21:55:20: 7000000 INFO @ Fri, 05 Jul 2019 21:55:20: 8000000 INFO @ Fri, 05 Jul 2019 21:55:25: 8000000 INFO @ Fri, 05 Jul 2019 21:55:28: 9000000 INFO @ Fri, 05 Jul 2019 21:55:29: 8000000 INFO @ Fri, 05 Jul 2019 21:55:35: 9000000 INFO @ Fri, 05 Jul 2019 21:55:36: 10000000 INFO @ Fri, 05 Jul 2019 21:55:39: 9000000 INFO @ Fri, 05 Jul 2019 21:55:40: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:55:40: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:55:40: #1 total tags in treatment: 10529162 INFO @ Fri, 05 Jul 2019 21:55:40: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:55:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:55:41: #1 tags after filtering in treatment: 10529162 INFO @ Fri, 05 Jul 2019 21:55:41: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:55:41: #1 finished! INFO @ Fri, 05 Jul 2019 21:55:41: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:55:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:55:41: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:55:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:55:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:55:44: 10000000 INFO @ Fri, 05 Jul 2019 21:55:49: 10000000 INFO @ Fri, 05 Jul 2019 21:55:49: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:55:49: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:55:49: #1 total tags in treatment: 10529162 INFO @ Fri, 05 Jul 2019 21:55:49: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:55:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:55:49: #1 tags after filtering in treatment: 10529162 INFO @ Fri, 05 Jul 2019 21:55:49: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:55:49: #1 finished! INFO @ Fri, 05 Jul 2019 21:55:49: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:55:49: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:55:50: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:55:50: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:55:50: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:55:53: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:55:53: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:55:53: #1 total tags in treatment: 10529162 INFO @ Fri, 05 Jul 2019 21:55:53: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:55:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:55:53: #1 tags after filtering in treatment: 10529162 INFO @ Fri, 05 Jul 2019 21:55:53: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:55:53: #1 finished! INFO @ Fri, 05 Jul 2019 21:55:53: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:55:53: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:55:54: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:55:54: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:55:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3294568/SRX3294568.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。