Job ID = 11192934 sra ファイルのダウンロード中... Completed: 597488K bytes transferred in 9 seconds (516375K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 18758366 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3242050/SRR6129612.sra Written 18758366 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3242050/SRR6129612.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:10 18758366 reads; of these: 18758366 (100.00%) were paired; of these: 18122246 (96.61%) aligned concordantly 0 times 530382 (2.83%) aligned concordantly exactly 1 time 105738 (0.56%) aligned concordantly >1 times ---- 18122246 pairs aligned concordantly 0 times; of these: 265711 (1.47%) aligned discordantly 1 time ---- 17856535 pairs aligned 0 times concordantly or discordantly; of these: 35713070 mates make up the pairs; of these: 32480129 (90.95%) aligned 0 times 2792441 (7.82%) aligned exactly 1 time 440500 (1.23%) aligned >1 times 13.42% overall alignment rate Time searching: 00:04:10 Overall time: 00:04:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 343441 / 898231 = 0.3824 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:40:17: # Command line: callpeak -t SRX3242050.bam -f BAM -g 12100000 -n SRX3242050.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3242050.05 # format = BAM # ChIP-seq file = ['SRX3242050.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:40:17: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:40:17: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:40:17: # Command line: callpeak -t SRX3242050.bam -f BAM -g 12100000 -n SRX3242050.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3242050.20 # format = BAM # ChIP-seq file = ['SRX3242050.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:40:17: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:40:17: # Command line: callpeak -t SRX3242050.bam -f BAM -g 12100000 -n SRX3242050.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3242050.10 # format = BAM # ChIP-seq file = ['SRX3242050.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:40:17: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:40:17: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:40:17: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:40:22: 1000000 INFO @ Sat, 15 Sep 2018 10:40:23: 1000000 INFO @ Sat, 15 Sep 2018 10:40:23: 1000000 INFO @ Sat, 15 Sep 2018 10:40:28: 2000000 INFO @ Sat, 15 Sep 2018 10:40:29: 2000000 INFO @ Sat, 15 Sep 2018 10:40:29: 2000000 INFO @ Sat, 15 Sep 2018 10:40:33: 3000000 INFO @ Sat, 15 Sep 2018 10:40:35: 3000000 INFO @ Sat, 15 Sep 2018 10:40:35: 3000000 INFO @ Sat, 15 Sep 2018 10:40:38: 4000000 INFO @ Sat, 15 Sep 2018 10:40:39: #1 tag size is determined as 42 bps INFO @ Sat, 15 Sep 2018 10:40:39: #1 tag size = 42 INFO @ Sat, 15 Sep 2018 10:40:39: #1 total tags in treatment: 422747 INFO @ Sat, 15 Sep 2018 10:40:39: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:40:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:40:39: #1 tags after filtering in treatment: 400910 INFO @ Sat, 15 Sep 2018 10:40:39: #1 Redundant rate of treatment: 0.05 INFO @ Sat, 15 Sep 2018 10:40:39: #1 finished! INFO @ Sat, 15 Sep 2018 10:40:39: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:40:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:40:40: #2 number of paired peaks: 35 WARNING @ Sat, 15 Sep 2018 10:40:40: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:40:40: Process for pairing-model is terminated! cat: SRX3242050.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3242050.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242050.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242050.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:40:40: 4000000 INFO @ Sat, 15 Sep 2018 10:40:40: 4000000 INFO @ Sat, 15 Sep 2018 10:40:42: #1 tag size is determined as 42 bps INFO @ Sat, 15 Sep 2018 10:40:42: #1 tag size = 42 INFO @ Sat, 15 Sep 2018 10:40:42: #1 total tags in treatment: 422747 INFO @ Sat, 15 Sep 2018 10:40:42: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:40:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:40:42: #1 tag size is determined as 42 bps INFO @ Sat, 15 Sep 2018 10:40:42: #1 tag size = 42 INFO @ Sat, 15 Sep 2018 10:40:42: #1 total tags in treatment: 422747 INFO @ Sat, 15 Sep 2018 10:40:42: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:40:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:40:42: #1 tags after filtering in treatment: 400910 INFO @ Sat, 15 Sep 2018 10:40:42: #1 Redundant rate of treatment: 0.05 INFO @ Sat, 15 Sep 2018 10:40:42: #1 finished! INFO @ Sat, 15 Sep 2018 10:40:42: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:40:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:40:42: #1 tags after filtering in treatment: 400910 INFO @ Sat, 15 Sep 2018 10:40:42: #1 Redundant rate of treatment: 0.05 INFO @ Sat, 15 Sep 2018 10:40:42: #1 finished! INFO @ Sat, 15 Sep 2018 10:40:42: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:40:42: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:40:42: #2 number of paired peaks: 35 WARNING @ Sat, 15 Sep 2018 10:40:42: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:40:42: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 10:40:42: #2 number of paired peaks: 35 WARNING @ Sat, 15 Sep 2018 10:40:42: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:40:42: Process for pairing-model is terminated! cat: SRX3242050.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX3242050.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3242050.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242050.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242050.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3242050.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242050.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242050.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。