Job ID = 11192927 sra ファイルのダウンロード中... Completed: 46893K bytes transferred in 3 seconds (110953K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 2060772 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3242042/SRR6129620.sra Written 2060772 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3242042/SRR6129620.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:17 2060772 reads; of these: 2060772 (100.00%) were unpaired; of these: 914644 (44.38%) aligned 0 times 1018259 (49.41%) aligned exactly 1 time 127869 (6.20%) aligned >1 times 55.62% overall alignment rate Time searching: 00:00:18 Overall time: 00:00:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 295093 / 1146128 = 0.2575 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:31:47: # Command line: callpeak -t SRX3242042.bam -f BAM -g 12100000 -n SRX3242042.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3242042.05 # format = BAM # ChIP-seq file = ['SRX3242042.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:31:47: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:31:47: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:31:47: # Command line: callpeak -t SRX3242042.bam -f BAM -g 12100000 -n SRX3242042.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3242042.10 # format = BAM # ChIP-seq file = ['SRX3242042.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:31:47: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:31:47: # Command line: callpeak -t SRX3242042.bam -f BAM -g 12100000 -n SRX3242042.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3242042.20 # format = BAM # ChIP-seq file = ['SRX3242042.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:31:47: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:31:47: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:31:47: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:31:52: #1 tag size is determined as 51 bps INFO @ Sat, 15 Sep 2018 10:31:52: #1 tag size is determined as 51 bps INFO @ Sat, 15 Sep 2018 10:31:52: #1 tag size = 51 INFO @ Sat, 15 Sep 2018 10:31:52: #1 tag size = 51 INFO @ Sat, 15 Sep 2018 10:31:52: #1 total tags in treatment: 851035 INFO @ Sat, 15 Sep 2018 10:31:52: #1 total tags in treatment: 851035 INFO @ Sat, 15 Sep 2018 10:31:52: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:31:52: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:31:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:31:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:31:52: #1 tags after filtering in treatment: 851035 INFO @ Sat, 15 Sep 2018 10:31:52: #1 tags after filtering in treatment: 851035 INFO @ Sat, 15 Sep 2018 10:31:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:31:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:31:52: #1 finished! INFO @ Sat, 15 Sep 2018 10:31:52: #1 finished! INFO @ Sat, 15 Sep 2018 10:31:52: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:31:52: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:31:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:31:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:31:52: #1 tag size is determined as 51 bps INFO @ Sat, 15 Sep 2018 10:31:52: #1 tag size = 51 INFO @ Sat, 15 Sep 2018 10:31:52: #1 total tags in treatment: 851035 INFO @ Sat, 15 Sep 2018 10:31:52: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:31:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:31:52: #1 tags after filtering in treatment: 851035 INFO @ Sat, 15 Sep 2018 10:31:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 15 Sep 2018 10:31:52: #1 finished! INFO @ Sat, 15 Sep 2018 10:31:52: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:31:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:31:52: #2 number of paired peaks: 54 WARNING @ Sat, 15 Sep 2018 10:31:52: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:31:52: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 10:31:52: #2 number of paired peaks: 54 WARNING @ Sat, 15 Sep 2018 10:31:52: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:31:52: Process for pairing-model is terminated! cat: cat: SRX3242042.10_peaks.narrowPeakSRX3242042.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3242042.10_model.r'rm: cannot remove `SRX3242042.20_model.r': そのようなファイルやディレクトリはありません : そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242042.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242042.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242042.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242042.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:31:53: #2 number of paired peaks: 54 WARNING @ Sat, 15 Sep 2018 10:31:53: Too few paired peaks (54) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:31:53: Process for pairing-model is terminated! cat: SRX3242042.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3242042.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242042.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242042.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。