Job ID = 11192918 sra ファイルのダウンロード中... Completed: 355591K bytes transferred in 6 seconds (443983K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 10964794 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3242033/SRR6129629.sra Written 10964794 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3242033/SRR6129629.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:11 10964794 reads; of these: 10964794 (100.00%) were paired; of these: 10487554 (95.65%) aligned concordantly 0 times 405887 (3.70%) aligned concordantly exactly 1 time 71353 (0.65%) aligned concordantly >1 times ---- 10487554 pairs aligned concordantly 0 times; of these: 125054 (1.19%) aligned discordantly 1 time ---- 10362500 pairs aligned 0 times concordantly or discordantly; of these: 20725000 mates make up the pairs; of these: 19261682 (92.94%) aligned 0 times 1262872 (6.09%) aligned exactly 1 time 200446 (0.97%) aligned >1 times 12.17% overall alignment rate Time searching: 00:02:11 Overall time: 00:02:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 184664 / 600422 = 0.3076 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:32:30: # Command line: callpeak -t SRX3242033.bam -f BAM -g 12100000 -n SRX3242033.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3242033.05 # format = BAM # ChIP-seq file = ['SRX3242033.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:32:30: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:32:30: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:32:30: # Command line: callpeak -t SRX3242033.bam -f BAM -g 12100000 -n SRX3242033.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3242033.20 # format = BAM # ChIP-seq file = ['SRX3242033.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:32:30: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:32:30: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:32:30: # Command line: callpeak -t SRX3242033.bam -f BAM -g 12100000 -n SRX3242033.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3242033.10 # format = BAM # ChIP-seq file = ['SRX3242033.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:32:30: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:32:30: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:32:34: 1000000 INFO @ Sat, 15 Sep 2018 10:32:34: 1000000 INFO @ Sat, 15 Sep 2018 10:32:34: 1000000 INFO @ Sat, 15 Sep 2018 10:32:39: 2000000 INFO @ Sat, 15 Sep 2018 10:32:39: 2000000 INFO @ Sat, 15 Sep 2018 10:32:39: 2000000 INFO @ Sat, 15 Sep 2018 10:32:40: #1 tag size is determined as 42 bps INFO @ Sat, 15 Sep 2018 10:32:40: #1 tag size = 42 INFO @ Sat, 15 Sep 2018 10:32:40: #1 total tags in treatment: 341964 INFO @ Sat, 15 Sep 2018 10:32:40: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:32:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:32:40: #1 tags after filtering in treatment: 327322 INFO @ Sat, 15 Sep 2018 10:32:40: #1 Redundant rate of treatment: 0.04 INFO @ Sat, 15 Sep 2018 10:32:40: #1 finished! INFO @ Sat, 15 Sep 2018 10:32:40: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:32:40: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:32:40: #2 number of paired peaks: 99 WARNING @ Sat, 15 Sep 2018 10:32:40: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:32:40: Process for pairing-model is terminated! cat: SRX3242033.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3242033.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242033.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242033.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:32:41: #1 tag size is determined as 42 bps INFO @ Sat, 15 Sep 2018 10:32:41: #1 tag size = 42 INFO @ Sat, 15 Sep 2018 10:32:41: #1 total tags in treatment: 341964 INFO @ Sat, 15 Sep 2018 10:32:41: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:32:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:32:41: #1 tags after filtering in treatment: 327322 INFO @ Sat, 15 Sep 2018 10:32:41: #1 Redundant rate of treatment: 0.04 INFO @ Sat, 15 Sep 2018 10:32:41: #1 finished! INFO @ Sat, 15 Sep 2018 10:32:41: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:32:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:32:41: #2 number of paired peaks: 99 WARNING @ Sat, 15 Sep 2018 10:32:41: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:32:41: Process for pairing-model is terminated! INFO @ Sat, 15 Sep 2018 10:32:41: #1 tag size is determined as 42 bps INFO @ Sat, 15 Sep 2018 10:32:41: #1 tag size = 42 INFO @ Sat, 15 Sep 2018 10:32:41: #1 total tags in treatment: 341964 INFO @ Sat, 15 Sep 2018 10:32:41: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:32:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) cat: SRX3242033.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) INFO @ Sat, 15 Sep 2018 10:32:41: #1 tags after filtering in treatment: 327322 INFO @ Sat, 15 Sep 2018 10:32:41: #1 Redundant rate of treatment: 0.04 INFO @ Sat, 15 Sep 2018 10:32:41: #1 finished! INFO @ Sat, 15 Sep 2018 10:32:41: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:32:41: #2 looking for paired plus/minus strand peaks... rm: cannot remove `SRX3242033.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242033.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242033.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:32:41: #2 number of paired peaks: 99 WARNING @ Sat, 15 Sep 2018 10:32:41: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 15 Sep 2018 10:32:41: Process for pairing-model is terminated! cat: SRX3242033.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3242033.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242033.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3242033.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。