Job ID = 11192912 sra ファイルのダウンロード中... Completed: 262973K bytes transferred in 5 seconds (387576K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 6669241 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3242027/SRR6129635.sra Written 6669241 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3242027/SRR6129635.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:05 6669241 reads; of these: 6669241 (100.00%) were paired; of these: 4973077 (74.57%) aligned concordantly 0 times 1034831 (15.52%) aligned concordantly exactly 1 time 661333 (9.92%) aligned concordantly >1 times ---- 4973077 pairs aligned concordantly 0 times; of these: 667414 (13.42%) aligned discordantly 1 time ---- 4305663 pairs aligned 0 times concordantly or discordantly; of these: 8611326 mates make up the pairs; of these: 5835572 (67.77%) aligned 0 times 2220019 (25.78%) aligned exactly 1 time 555735 (6.45%) aligned >1 times 56.25% overall alignment rate Time searching: 00:05:05 Overall time: 00:05:05 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 430395 / 2056104 = 0.2093 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:30:04: # Command line: callpeak -t SRX3242027.bam -f BAM -g 12100000 -n SRX3242027.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3242027.20 # format = BAM # ChIP-seq file = ['SRX3242027.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:30:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:30:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:30:04: # Command line: callpeak -t SRX3242027.bam -f BAM -g 12100000 -n SRX3242027.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3242027.05 # format = BAM # ChIP-seq file = ['SRX3242027.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:30:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:30:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:30:04: # Command line: callpeak -t SRX3242027.bam -f BAM -g 12100000 -n SRX3242027.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3242027.10 # format = BAM # ChIP-seq file = ['SRX3242027.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:30:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:30:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:30:09: 1000000 INFO @ Sat, 15 Sep 2018 10:30:09: 1000000 INFO @ Sat, 15 Sep 2018 10:30:09: 1000000 INFO @ Sat, 15 Sep 2018 10:30:13: 2000000 INFO @ Sat, 15 Sep 2018 10:30:13: 2000000 INFO @ Sat, 15 Sep 2018 10:30:13: 2000000 INFO @ Sat, 15 Sep 2018 10:30:18: 3000000 INFO @ Sat, 15 Sep 2018 10:30:18: 3000000 INFO @ Sat, 15 Sep 2018 10:30:18: 3000000 INFO @ Sat, 15 Sep 2018 10:30:23: 4000000 INFO @ Sat, 15 Sep 2018 10:30:23: 4000000 INFO @ Sat, 15 Sep 2018 10:30:23: 4000000 INFO @ Sat, 15 Sep 2018 10:30:27: 5000000 INFO @ Sat, 15 Sep 2018 10:30:27: 5000000 INFO @ Sat, 15 Sep 2018 10:30:28: 5000000 INFO @ Sat, 15 Sep 2018 10:30:32: 6000000 INFO @ Sat, 15 Sep 2018 10:30:32: 6000000 INFO @ Sat, 15 Sep 2018 10:30:33: 6000000 INFO @ Sat, 15 Sep 2018 10:30:35: #1 tag size is determined as 42 bps INFO @ Sat, 15 Sep 2018 10:30:35: #1 tag size = 42 INFO @ Sat, 15 Sep 2018 10:30:35: #1 total tags in treatment: 1440286 INFO @ Sat, 15 Sep 2018 10:30:35: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:30:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:30:35: #1 tag size is determined as 42 bps INFO @ Sat, 15 Sep 2018 10:30:35: #1 tag size = 42 INFO @ Sat, 15 Sep 2018 10:30:35: #1 total tags in treatment: 1440286 INFO @ Sat, 15 Sep 2018 10:30:35: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:30:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:30:35: #1 tags after filtering in treatment: 997505 INFO @ Sat, 15 Sep 2018 10:30:35: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Sep 2018 10:30:35: #1 finished! INFO @ Sat, 15 Sep 2018 10:30:35: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:30:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:30:35: #1 tags after filtering in treatment: 997505 INFO @ Sat, 15 Sep 2018 10:30:35: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Sep 2018 10:30:35: #1 finished! INFO @ Sat, 15 Sep 2018 10:30:35: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:30:35: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:30:35: #2 number of paired peaks: 727 WARNING @ Sat, 15 Sep 2018 10:30:35: Fewer paired peaks (727) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 727 pairs to build model! INFO @ Sat, 15 Sep 2018 10:30:35: start model_add_line... INFO @ Sat, 15 Sep 2018 10:30:35: #2 number of paired peaks: 727 WARNING @ Sat, 15 Sep 2018 10:30:35: Fewer paired peaks (727) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 727 pairs to build model! INFO @ Sat, 15 Sep 2018 10:30:35: start model_add_line... INFO @ Sat, 15 Sep 2018 10:30:35: start X-correlation... INFO @ Sat, 15 Sep 2018 10:30:35: end of X-cor INFO @ Sat, 15 Sep 2018 10:30:35: #2 finished! INFO @ Sat, 15 Sep 2018 10:30:35: #2 predicted fragment length is 166 bps INFO @ Sat, 15 Sep 2018 10:30:35: #2 alternative fragment length(s) may be 4,166 bps INFO @ Sat, 15 Sep 2018 10:30:35: #2.2 Generate R script for model : SRX3242027.20_model.r INFO @ Sat, 15 Sep 2018 10:30:35: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:30:35: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:30:35: start X-correlation... INFO @ Sat, 15 Sep 2018 10:30:35: end of X-cor INFO @ Sat, 15 Sep 2018 10:30:35: #2 finished! INFO @ Sat, 15 Sep 2018 10:30:35: #2 predicted fragment length is 166 bps INFO @ Sat, 15 Sep 2018 10:30:35: #2 alternative fragment length(s) may be 4,166 bps INFO @ Sat, 15 Sep 2018 10:30:35: #2.2 Generate R script for model : SRX3242027.05_model.r INFO @ Sat, 15 Sep 2018 10:30:35: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:30:35: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:30:36: #1 tag size is determined as 42 bps INFO @ Sat, 15 Sep 2018 10:30:36: #1 tag size = 42 INFO @ Sat, 15 Sep 2018 10:30:36: #1 total tags in treatment: 1440286 INFO @ Sat, 15 Sep 2018 10:30:36: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:30:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:30:36: #1 tags after filtering in treatment: 997505 INFO @ Sat, 15 Sep 2018 10:30:36: #1 Redundant rate of treatment: 0.31 INFO @ Sat, 15 Sep 2018 10:30:36: #1 finished! INFO @ Sat, 15 Sep 2018 10:30:36: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:30:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:30:36: #2 number of paired peaks: 727 WARNING @ Sat, 15 Sep 2018 10:30:36: Fewer paired peaks (727) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 727 pairs to build model! INFO @ Sat, 15 Sep 2018 10:30:36: start model_add_line... INFO @ Sat, 15 Sep 2018 10:30:36: start X-correlation... INFO @ Sat, 15 Sep 2018 10:30:36: end of X-cor INFO @ Sat, 15 Sep 2018 10:30:36: #2 finished! INFO @ Sat, 15 Sep 2018 10:30:36: #2 predicted fragment length is 166 bps INFO @ Sat, 15 Sep 2018 10:30:36: #2 alternative fragment length(s) may be 4,166 bps INFO @ Sat, 15 Sep 2018 10:30:36: #2.2 Generate R script for model : SRX3242027.10_model.r INFO @ Sat, 15 Sep 2018 10:30:36: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:30:36: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:30:40: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:30:40: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:30:41: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:30:41: #4 Write output xls file... SRX3242027.20_peaks.xls INFO @ Sat, 15 Sep 2018 10:30:41: #4 Write peak in narrowPeak format file... SRX3242027.20_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:30:41: #4 Write summits bed file... SRX3242027.20_summits.bed INFO @ Sat, 15 Sep 2018 10:30:41: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (222 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:30:41: #4 Write output xls file... SRX3242027.05_peaks.xls INFO @ Sat, 15 Sep 2018 10:30:41: #4 Write peak in narrowPeak format file... SRX3242027.05_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:30:41: #4 Write summits bed file... SRX3242027.05_summits.bed INFO @ Sat, 15 Sep 2018 10:30:41: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (398 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:30:42: #4 Write output xls file... SRX3242027.10_peaks.xls INFO @ Sat, 15 Sep 2018 10:30:42: #4 Write peak in narrowPeak format file... SRX3242027.10_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:30:42: #4 Write summits bed file... SRX3242027.10_summits.bed INFO @ Sat, 15 Sep 2018 10:30:42: Done! pass1 - making usageList (17 chroms): 1 millis pass2 - checking and writing primary data (312 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。