Job ID = 5790842 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 15,275,580 reads read : 15,275,580 reads written : 15,275,580 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6055810.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:42 15275580 reads; of these: 15275580 (100.00%) were unpaired; of these: 999990 (6.55%) aligned 0 times 8908886 (58.32%) aligned exactly 1 time 5366704 (35.13%) aligned >1 times 93.45% overall alignment rate Time searching: 00:01:42 Overall time: 00:01:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7118530 / 14275590 = 0.4987 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:54:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:54:51: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:54:51: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:54:55: 1000000 INFO @ Wed, 22 Apr 2020 07:55:00: 2000000 INFO @ Wed, 22 Apr 2020 07:55:05: 3000000 INFO @ Wed, 22 Apr 2020 07:55:10: 4000000 INFO @ Wed, 22 Apr 2020 07:55:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:55:20: 6000000 INFO @ Wed, 22 Apr 2020 07:55:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:55:21: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:55:21: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:55:25: 7000000 INFO @ Wed, 22 Apr 2020 07:55:26: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:55:26: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:55:26: #1 total tags in treatment: 7157060 INFO @ Wed, 22 Apr 2020 07:55:26: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:55:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:55:26: #1 tags after filtering in treatment: 7157060 INFO @ Wed, 22 Apr 2020 07:55:26: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:55:26: #1 finished! INFO @ Wed, 22 Apr 2020 07:55:26: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:55:26: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:55:26: 1000000 INFO @ Wed, 22 Apr 2020 07:55:26: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:55:26: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:55:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:55:31: 2000000 INFO @ Wed, 22 Apr 2020 07:55:36: 3000000 INFO @ Wed, 22 Apr 2020 07:55:41: 4000000 INFO @ Wed, 22 Apr 2020 07:55:46: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:55:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:55:51: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:55:51: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:55:51: 6000000 INFO @ Wed, 22 Apr 2020 07:55:56: 1000000 INFO @ Wed, 22 Apr 2020 07:55:56: 7000000 INFO @ Wed, 22 Apr 2020 07:55:57: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:55:57: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:55:57: #1 total tags in treatment: 7157060 INFO @ Wed, 22 Apr 2020 07:55:57: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:55:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:55:57: #1 tags after filtering in treatment: 7157060 INFO @ Wed, 22 Apr 2020 07:55:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:55:57: #1 finished! INFO @ Wed, 22 Apr 2020 07:55:57: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:55:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:55:58: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:55:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:55:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:56:01: 2000000 INFO @ Wed, 22 Apr 2020 07:56:06: 3000000 INFO @ Wed, 22 Apr 2020 07:56:11: 4000000 INFO @ Wed, 22 Apr 2020 07:56:16: 5000000 INFO @ Wed, 22 Apr 2020 07:56:21: 6000000 INFO @ Wed, 22 Apr 2020 07:56:26: 7000000 INFO @ Wed, 22 Apr 2020 07:56:27: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:56:27: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:56:27: #1 total tags in treatment: 7157060 INFO @ Wed, 22 Apr 2020 07:56:27: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:56:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:56:27: #1 tags after filtering in treatment: 7157060 INFO @ Wed, 22 Apr 2020 07:56:27: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:56:27: #1 finished! INFO @ Wed, 22 Apr 2020 07:56:27: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:56:27: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:56:28: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:56:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:56:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202739/SRX3202739.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。