Job ID = 5790840 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 24,276,493 reads read : 24,276,493 reads written : 24,276,493 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6055675.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:42 24276493 reads; of these: 24276493 (100.00%) were unpaired; of these: 647006 (2.67%) aligned 0 times 16593890 (68.35%) aligned exactly 1 time 7035597 (28.98%) aligned >1 times 97.33% overall alignment rate Time searching: 00:02:42 Overall time: 00:02:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13451807 / 23629487 = 0.5693 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:56:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:56:42: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:56:42: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:56:47: 1000000 INFO @ Wed, 22 Apr 2020 07:56:51: 2000000 INFO @ Wed, 22 Apr 2020 07:56:56: 3000000 INFO @ Wed, 22 Apr 2020 07:57:01: 4000000 INFO @ Wed, 22 Apr 2020 07:57:06: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:57:10: 6000000 INFO @ Wed, 22 Apr 2020 07:57:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:57:12: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:57:12: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:57:15: 7000000 INFO @ Wed, 22 Apr 2020 07:57:17: 1000000 INFO @ Wed, 22 Apr 2020 07:57:20: 8000000 INFO @ Wed, 22 Apr 2020 07:57:22: 2000000 INFO @ Wed, 22 Apr 2020 07:57:25: 9000000 INFO @ Wed, 22 Apr 2020 07:57:27: 3000000 INFO @ Wed, 22 Apr 2020 07:57:30: 10000000 INFO @ Wed, 22 Apr 2020 07:57:31: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 07:57:31: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 07:57:31: #1 total tags in treatment: 10177680 INFO @ Wed, 22 Apr 2020 07:57:31: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:57:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:57:32: #1 tags after filtering in treatment: 10177680 INFO @ Wed, 22 Apr 2020 07:57:32: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:57:32: #1 finished! INFO @ Wed, 22 Apr 2020 07:57:32: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:57:32: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:57:32: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:57:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:57:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:57:32: 4000000 INFO @ Wed, 22 Apr 2020 07:57:37: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:57:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:57:42: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:57:42: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:57:42: 6000000 INFO @ Wed, 22 Apr 2020 07:57:47: 1000000 INFO @ Wed, 22 Apr 2020 07:57:47: 7000000 INFO @ Wed, 22 Apr 2020 07:57:52: 2000000 INFO @ Wed, 22 Apr 2020 07:57:53: 8000000 INFO @ Wed, 22 Apr 2020 07:57:57: 3000000 INFO @ Wed, 22 Apr 2020 07:57:58: 9000000 INFO @ Wed, 22 Apr 2020 07:58:02: 4000000 INFO @ Wed, 22 Apr 2020 07:58:03: 10000000 INFO @ Wed, 22 Apr 2020 07:58:04: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 07:58:04: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 07:58:04: #1 total tags in treatment: 10177680 INFO @ Wed, 22 Apr 2020 07:58:04: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:58:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:58:04: #1 tags after filtering in treatment: 10177680 INFO @ Wed, 22 Apr 2020 07:58:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:58:04: #1 finished! INFO @ Wed, 22 Apr 2020 07:58:04: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:58:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:58:04: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:58:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:58:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:58:08: 5000000 INFO @ Wed, 22 Apr 2020 07:58:13: 6000000 INFO @ Wed, 22 Apr 2020 07:58:17: 7000000 INFO @ Wed, 22 Apr 2020 07:58:22: 8000000 INFO @ Wed, 22 Apr 2020 07:58:27: 9000000 INFO @ Wed, 22 Apr 2020 07:58:32: 10000000 INFO @ Wed, 22 Apr 2020 07:58:33: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 07:58:33: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 07:58:33: #1 total tags in treatment: 10177680 INFO @ Wed, 22 Apr 2020 07:58:33: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:58:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:58:33: #1 tags after filtering in treatment: 10177680 INFO @ Wed, 22 Apr 2020 07:58:33: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:58:33: #1 finished! INFO @ Wed, 22 Apr 2020 07:58:33: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:58:33: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:58:34: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:58:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:58:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202604/SRX3202604.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。