Job ID = 5790838 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 21,289,976 reads read : 21,289,976 reads written : 21,289,976 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6055674.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:15 21289976 reads; of these: 21289976 (100.00%) were unpaired; of these: 423426 (1.99%) aligned 0 times 16005889 (75.18%) aligned exactly 1 time 4860661 (22.83%) aligned >1 times 98.01% overall alignment rate Time searching: 00:02:15 Overall time: 00:02:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 9447094 / 20866550 = 0.4527 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:55:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:55:11: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:55:11: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:55:16: 1000000 INFO @ Wed, 22 Apr 2020 07:55:21: 2000000 INFO @ Wed, 22 Apr 2020 07:55:27: 3000000 INFO @ Wed, 22 Apr 2020 07:55:32: 4000000 INFO @ Wed, 22 Apr 2020 07:55:37: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:55:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:55:41: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:55:41: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:55:42: 6000000 INFO @ Wed, 22 Apr 2020 07:55:47: 1000000 INFO @ Wed, 22 Apr 2020 07:55:48: 7000000 INFO @ Wed, 22 Apr 2020 07:55:52: 2000000 INFO @ Wed, 22 Apr 2020 07:55:54: 8000000 INFO @ Wed, 22 Apr 2020 07:55:58: 3000000 INFO @ Wed, 22 Apr 2020 07:55:59: 9000000 INFO @ Wed, 22 Apr 2020 07:56:04: 4000000 INFO @ Wed, 22 Apr 2020 07:56:05: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:56:10: 5000000 INFO @ Wed, 22 Apr 2020 07:56:11: 11000000 INFO @ Wed, 22 Apr 2020 07:56:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:56:11: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:56:11: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:56:13: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 07:56:13: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 07:56:13: #1 total tags in treatment: 11419456 INFO @ Wed, 22 Apr 2020 07:56:13: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:56:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:56:13: #1 tags after filtering in treatment: 11419456 INFO @ Wed, 22 Apr 2020 07:56:13: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:56:13: #1 finished! INFO @ Wed, 22 Apr 2020 07:56:13: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:56:13: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:56:14: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:56:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:56:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:56:16: 6000000 INFO @ Wed, 22 Apr 2020 07:56:17: 1000000 INFO @ Wed, 22 Apr 2020 07:56:21: 7000000 INFO @ Wed, 22 Apr 2020 07:56:23: 2000000 INFO @ Wed, 22 Apr 2020 07:56:27: 8000000 INFO @ Wed, 22 Apr 2020 07:56:28: 3000000 INFO @ Wed, 22 Apr 2020 07:56:33: 9000000 INFO @ Wed, 22 Apr 2020 07:56:34: 4000000 INFO @ Wed, 22 Apr 2020 07:56:39: 10000000 INFO @ Wed, 22 Apr 2020 07:56:40: 5000000 INFO @ Wed, 22 Apr 2020 07:56:44: 11000000 INFO @ Wed, 22 Apr 2020 07:56:46: 6000000 INFO @ Wed, 22 Apr 2020 07:56:47: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 07:56:47: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 07:56:47: #1 total tags in treatment: 11419456 INFO @ Wed, 22 Apr 2020 07:56:47: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:56:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:56:47: #1 tags after filtering in treatment: 11419456 INFO @ Wed, 22 Apr 2020 07:56:47: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:56:47: #1 finished! INFO @ Wed, 22 Apr 2020 07:56:47: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:56:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:56:47: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:56:47: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:56:47: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:56:52: 7000000 INFO @ Wed, 22 Apr 2020 07:56:57: 8000000 INFO @ Wed, 22 Apr 2020 07:57:03: 9000000 INFO @ Wed, 22 Apr 2020 07:57:09: 10000000 INFO @ Wed, 22 Apr 2020 07:57:14: 11000000 INFO @ Wed, 22 Apr 2020 07:57:17: #1 tag size is determined as 50 bps INFO @ Wed, 22 Apr 2020 07:57:17: #1 tag size = 50 INFO @ Wed, 22 Apr 2020 07:57:17: #1 total tags in treatment: 11419456 INFO @ Wed, 22 Apr 2020 07:57:17: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:57:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:57:17: #1 tags after filtering in treatment: 11419456 INFO @ Wed, 22 Apr 2020 07:57:17: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:57:17: #1 finished! INFO @ Wed, 22 Apr 2020 07:57:17: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:57:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:57:18: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:57:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:57:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3202603/SRX3202603.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。