Job ID = 5790835 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 12,171,484 reads read : 12,171,484 reads written : 12,171,484 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6050730.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:24 12171484 reads; of these: 12171484 (100.00%) were unpaired; of these: 784718 (6.45%) aligned 0 times 8837971 (72.61%) aligned exactly 1 time 2548795 (20.94%) aligned >1 times 93.55% overall alignment rate Time searching: 00:01:24 Overall time: 00:01:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5143911 / 11386766 = 0.4517 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:50:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:50:15: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:50:15: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:50:20: 1000000 INFO @ Wed, 22 Apr 2020 07:50:24: 2000000 INFO @ Wed, 22 Apr 2020 07:50:29: 3000000 INFO @ Wed, 22 Apr 2020 07:50:33: 4000000 INFO @ Wed, 22 Apr 2020 07:50:37: 5000000 INFO @ Wed, 22 Apr 2020 07:50:42: 6000000 INFO @ Wed, 22 Apr 2020 07:50:43: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:50:43: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:50:43: #1 total tags in treatment: 6242855 INFO @ Wed, 22 Apr 2020 07:50:43: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:50:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:50:43: #1 tags after filtering in treatment: 6242855 INFO @ Wed, 22 Apr 2020 07:50:43: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:50:43: #1 finished! INFO @ Wed, 22 Apr 2020 07:50:43: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:50:43: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:50:43: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:50:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:50:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:50:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:50:45: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:50:45: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:50:50: 1000000 INFO @ Wed, 22 Apr 2020 07:50:54: 2000000 INFO @ Wed, 22 Apr 2020 07:50:59: 3000000 INFO @ Wed, 22 Apr 2020 07:51:03: 4000000 INFO @ Wed, 22 Apr 2020 07:51:07: 5000000 INFO @ Wed, 22 Apr 2020 07:51:12: 6000000 INFO @ Wed, 22 Apr 2020 07:51:13: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:51:13: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:51:13: #1 total tags in treatment: 6242855 INFO @ Wed, 22 Apr 2020 07:51:13: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:51:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:51:13: #1 tags after filtering in treatment: 6242855 INFO @ Wed, 22 Apr 2020 07:51:13: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:51:13: #1 finished! INFO @ Wed, 22 Apr 2020 07:51:13: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:51:13: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:51:13: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:51:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:51:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:51:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:51:15: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:51:15: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:51:20: 1000000 INFO @ Wed, 22 Apr 2020 07:51:25: 2000000 INFO @ Wed, 22 Apr 2020 07:51:29: 3000000 INFO @ Wed, 22 Apr 2020 07:51:34: 4000000 INFO @ Wed, 22 Apr 2020 07:51:38: 5000000 INFO @ Wed, 22 Apr 2020 07:51:43: 6000000 INFO @ Wed, 22 Apr 2020 07:51:44: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:51:44: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:51:44: #1 total tags in treatment: 6242855 INFO @ Wed, 22 Apr 2020 07:51:44: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:51:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:51:44: #1 tags after filtering in treatment: 6242855 INFO @ Wed, 22 Apr 2020 07:51:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:51:44: #1 finished! INFO @ Wed, 22 Apr 2020 07:51:44: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:51:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:51:44: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:51:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:51:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197697/SRX3197697.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。