Job ID = 5790833 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,801,194 reads read : 16,801,194 reads written : 16,801,194 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6050729.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:55 16801194 reads; of these: 16801194 (100.00%) were unpaired; of these: 502418 (2.99%) aligned 0 times 12427706 (73.97%) aligned exactly 1 time 3871070 (23.04%) aligned >1 times 97.01% overall alignment rate Time searching: 00:01:55 Overall time: 00:01:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6932333 / 16298776 = 0.4253 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:52:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:52:12: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:52:12: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:52:17: 1000000 INFO @ Wed, 22 Apr 2020 07:52:23: 2000000 INFO @ Wed, 22 Apr 2020 07:52:28: 3000000 INFO @ Wed, 22 Apr 2020 07:52:33: 4000000 INFO @ Wed, 22 Apr 2020 07:52:39: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:52:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:52:42: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:52:42: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:52:44: 6000000 INFO @ Wed, 22 Apr 2020 07:52:47: 1000000 INFO @ Wed, 22 Apr 2020 07:52:50: 7000000 INFO @ Wed, 22 Apr 2020 07:52:52: 2000000 INFO @ Wed, 22 Apr 2020 07:52:56: 8000000 INFO @ Wed, 22 Apr 2020 07:52:57: 3000000 INFO @ Wed, 22 Apr 2020 07:53:01: 9000000 INFO @ Wed, 22 Apr 2020 07:53:02: 4000000 INFO @ Wed, 22 Apr 2020 07:53:03: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:53:03: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:53:03: #1 total tags in treatment: 9366443 INFO @ Wed, 22 Apr 2020 07:53:03: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:53:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:53:03: #1 tags after filtering in treatment: 9366443 INFO @ Wed, 22 Apr 2020 07:53:03: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:53:03: #1 finished! INFO @ Wed, 22 Apr 2020 07:53:03: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:53:03: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:53:04: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:53:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:53:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:53:07: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:53:12: 6000000 INFO @ Wed, 22 Apr 2020 07:53:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:53:12: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:53:12: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:53:17: 7000000 INFO @ Wed, 22 Apr 2020 07:53:18: 1000000 INFO @ Wed, 22 Apr 2020 07:53:22: 8000000 INFO @ Wed, 22 Apr 2020 07:53:23: 2000000 INFO @ Wed, 22 Apr 2020 07:53:27: 9000000 INFO @ Wed, 22 Apr 2020 07:53:29: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:53:29: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:53:29: #1 total tags in treatment: 9366443 INFO @ Wed, 22 Apr 2020 07:53:29: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:53:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:53:29: #1 tags after filtering in treatment: 9366443 INFO @ Wed, 22 Apr 2020 07:53:29: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:53:29: #1 finished! INFO @ Wed, 22 Apr 2020 07:53:29: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:53:29: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:53:29: 3000000 INFO @ Wed, 22 Apr 2020 07:53:29: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:53:29: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:53:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:53:34: 4000000 INFO @ Wed, 22 Apr 2020 07:53:40: 5000000 INFO @ Wed, 22 Apr 2020 07:53:45: 6000000 INFO @ Wed, 22 Apr 2020 07:53:51: 7000000 INFO @ Wed, 22 Apr 2020 07:53:56: 8000000 INFO @ Wed, 22 Apr 2020 07:54:01: 9000000 INFO @ Wed, 22 Apr 2020 07:54:03: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:54:03: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:54:03: #1 total tags in treatment: 9366443 INFO @ Wed, 22 Apr 2020 07:54:03: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:54:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:54:04: #1 tags after filtering in treatment: 9366443 INFO @ Wed, 22 Apr 2020 07:54:04: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:54:04: #1 finished! INFO @ Wed, 22 Apr 2020 07:54:04: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:54:04: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:54:04: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:54:04: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:54:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197696/SRX3197696.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。