Job ID = 5790830 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 17,354,601 reads read : 17,354,601 reads written : 17,354,601 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘/home/okishinya/ncbi/public/sra/SRR6050727.sra.cache’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:10 17354601 reads; of these: 17354601 (100.00%) were unpaired; of these: 364808 (2.10%) aligned 0 times 14180712 (81.71%) aligned exactly 1 time 2809081 (16.19%) aligned >1 times 97.90% overall alignment rate Time searching: 00:02:10 Overall time: 00:02:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6285976 / 16989793 = 0.3700 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:51:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:51:23: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:51:23: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:51:28: 1000000 INFO @ Wed, 22 Apr 2020 07:51:32: 2000000 INFO @ Wed, 22 Apr 2020 07:51:36: 3000000 INFO @ Wed, 22 Apr 2020 07:51:41: 4000000 INFO @ Wed, 22 Apr 2020 07:51:45: 5000000 INFO @ Wed, 22 Apr 2020 07:51:50: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:51:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:51:53: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:51:53: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:51:54: 7000000 INFO @ Wed, 22 Apr 2020 07:51:58: 8000000 INFO @ Wed, 22 Apr 2020 07:51:59: 1000000 INFO @ Wed, 22 Apr 2020 07:52:03: 9000000 INFO @ Wed, 22 Apr 2020 07:52:04: 2000000 INFO @ Wed, 22 Apr 2020 07:52:07: 10000000 INFO @ Wed, 22 Apr 2020 07:52:09: 3000000 INFO @ Wed, 22 Apr 2020 07:52:10: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:52:10: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:52:10: #1 total tags in treatment: 10703817 INFO @ Wed, 22 Apr 2020 07:52:10: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:52:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:52:11: #1 tags after filtering in treatment: 10703817 INFO @ Wed, 22 Apr 2020 07:52:11: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:52:11: #1 finished! INFO @ Wed, 22 Apr 2020 07:52:11: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:52:11: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:52:11: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:52:11: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:52:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:52:14: 4000000 INFO @ Wed, 22 Apr 2020 07:52:19: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Apr 2020 07:52:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Apr 2020 07:52:23: #1 read tag files... INFO @ Wed, 22 Apr 2020 07:52:23: #1 read treatment tags... INFO @ Wed, 22 Apr 2020 07:52:24: 6000000 INFO @ Wed, 22 Apr 2020 07:52:28: 1000000 INFO @ Wed, 22 Apr 2020 07:52:29: 7000000 INFO @ Wed, 22 Apr 2020 07:52:33: 2000000 INFO @ Wed, 22 Apr 2020 07:52:34: 8000000 INFO @ Wed, 22 Apr 2020 07:52:38: 3000000 INFO @ Wed, 22 Apr 2020 07:52:39: 9000000 INFO @ Wed, 22 Apr 2020 07:52:43: 4000000 INFO @ Wed, 22 Apr 2020 07:52:44: 10000000 INFO @ Wed, 22 Apr 2020 07:52:47: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:52:47: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:52:47: #1 total tags in treatment: 10703817 INFO @ Wed, 22 Apr 2020 07:52:47: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:52:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:52:47: #1 tags after filtering in treatment: 10703817 INFO @ Wed, 22 Apr 2020 07:52:47: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:52:47: #1 finished! INFO @ Wed, 22 Apr 2020 07:52:47: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:52:47: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:52:48: 5000000 INFO @ Wed, 22 Apr 2020 07:52:48: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:52:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:52:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Wed, 22 Apr 2020 07:52:53: 6000000 INFO @ Wed, 22 Apr 2020 07:52:58: 7000000 INFO @ Wed, 22 Apr 2020 07:53:03: 8000000 INFO @ Wed, 22 Apr 2020 07:53:08: 9000000 INFO @ Wed, 22 Apr 2020 07:53:13: 10000000 INFO @ Wed, 22 Apr 2020 07:53:16: #1 tag size is determined as 51 bps INFO @ Wed, 22 Apr 2020 07:53:16: #1 tag size = 51 INFO @ Wed, 22 Apr 2020 07:53:16: #1 total tags in treatment: 10703817 INFO @ Wed, 22 Apr 2020 07:53:16: #1 user defined the maximum tags... INFO @ Wed, 22 Apr 2020 07:53:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Apr 2020 07:53:17: #1 tags after filtering in treatment: 10703817 INFO @ Wed, 22 Apr 2020 07:53:17: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Apr 2020 07:53:17: #1 finished! INFO @ Wed, 22 Apr 2020 07:53:17: #2 Build Peak Model... INFO @ Wed, 22 Apr 2020 07:53:17: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Apr 2020 07:53:17: #2 number of paired peaks: 0 WARNING @ Wed, 22 Apr 2020 07:53:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Apr 2020 07:53:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX3197694/SRX3197694.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。