Job ID = 10609004 sra ファイルのダウンロード中... Completed: 219260K bytes transferred in 7 seconds (229272K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Written 2545293 spots for /home/okishinya/chipatlas/results/sacCer3/SRX3063092/SRR5901522.sra Written 2545293 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:45 2545293 reads; of these: 2545293 (100.00%) were paired; of these: 180900 (7.11%) aligned concordantly 0 times 2029379 (79.73%) aligned concordantly exactly 1 time 335014 (13.16%) aligned concordantly >1 times ---- 180900 pairs aligned concordantly 0 times; of these: 30408 (16.81%) aligned discordantly 1 time ---- 150492 pairs aligned 0 times concordantly or discordantly; of these: 300984 mates make up the pairs; of these: 255673 (84.95%) aligned 0 times 30133 (10.01%) aligned exactly 1 time 15178 (5.04%) aligned >1 times 94.98% overall alignment rate Time searching: 00:02:45 Overall time: 00:02:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 32850 / 2392422 = 0.0137 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Thu, 03 May 2018 23:05:38: # Command line: callpeak -t SRX3063092.bam -f BAM -g 12100000 -n SRX3063092.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX3063092.05 # format = BAM # ChIP-seq file = ['SRX3063092.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:05:38: #1 read tag files... INFO @ Thu, 03 May 2018 23:05:38: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:05:38: # Command line: callpeak -t SRX3063092.bam -f BAM -g 12100000 -n SRX3063092.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX3063092.10 # format = BAM # ChIP-seq file = ['SRX3063092.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:05:38: #1 read tag files... INFO @ Thu, 03 May 2018 23:05:38: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:05:38: # Command line: callpeak -t SRX3063092.bam -f BAM -g 12100000 -n SRX3063092.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX3063092.20 # format = BAM # ChIP-seq file = ['SRX3063092.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Thu, 03 May 2018 23:05:38: #1 read tag files... INFO @ Thu, 03 May 2018 23:05:38: #1 read treatment tags... INFO @ Thu, 03 May 2018 23:05:49: 1000000 INFO @ Thu, 03 May 2018 23:05:49: 1000000 INFO @ Thu, 03 May 2018 23:05:49: 1000000 INFO @ Thu, 03 May 2018 23:05:59: 2000000 INFO @ Thu, 03 May 2018 23:05:59: 2000000 INFO @ Thu, 03 May 2018 23:05:59: 2000000 INFO @ Thu, 03 May 2018 23:06:10: 3000000 INFO @ Thu, 03 May 2018 23:06:10: 3000000 INFO @ Thu, 03 May 2018 23:06:10: 3000000 INFO @ Thu, 03 May 2018 23:06:18: 4000000 INFO @ Thu, 03 May 2018 23:06:18: 4000000 INFO @ Thu, 03 May 2018 23:06:18: 4000000 INFO @ Thu, 03 May 2018 23:06:24: #1 tag size is determined as 79 bps INFO @ Thu, 03 May 2018 23:06:24: #1 tag size = 79 INFO @ Thu, 03 May 2018 23:06:24: #1 total tags in treatment: 2331706 INFO @ Thu, 03 May 2018 23:06:24: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:06:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:06:24: #1 tag size is determined as 79 bps INFO @ Thu, 03 May 2018 23:06:24: #1 tag size = 79 INFO @ Thu, 03 May 2018 23:06:24: #1 total tags in treatment: 2331706 INFO @ Thu, 03 May 2018 23:06:24: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:06:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:06:24: #1 tags after filtering in treatment: 2082090 INFO @ Thu, 03 May 2018 23:06:24: #1 Redundant rate of treatment: 0.11 INFO @ Thu, 03 May 2018 23:06:24: #1 finished! INFO @ Thu, 03 May 2018 23:06:24: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:06:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:06:24: #1 tags after filtering in treatment: 2082090 INFO @ Thu, 03 May 2018 23:06:24: #1 Redundant rate of treatment: 0.11 INFO @ Thu, 03 May 2018 23:06:24: #1 finished! INFO @ Thu, 03 May 2018 23:06:24: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:06:24: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:06:24: #2 number of paired peaks: 36 WARNING @ Thu, 03 May 2018 23:06:24: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:06:24: Process for pairing-model is terminated! INFO @ Thu, 03 May 2018 23:06:24: #2 number of paired peaks: 36 WARNING @ Thu, 03 May 2018 23:06:24: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:06:24: Process for pairing-model is terminated! cat: SRX3063092.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX3063092.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3063092.20_model.r': そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis rm: cannot remove `SRX3063092.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3063092.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3063092.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3063092.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3063092.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Thu, 03 May 2018 23:06:25: #1 tag size is determined as 79 bps INFO @ Thu, 03 May 2018 23:06:25: #1 tag size = 79 INFO @ Thu, 03 May 2018 23:06:25: #1 total tags in treatment: 2331706 INFO @ Thu, 03 May 2018 23:06:25: #1 user defined the maximum tags... INFO @ Thu, 03 May 2018 23:06:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Thu, 03 May 2018 23:06:25: #1 tags after filtering in treatment: 2082090 INFO @ Thu, 03 May 2018 23:06:25: #1 Redundant rate of treatment: 0.11 INFO @ Thu, 03 May 2018 23:06:25: #1 finished! INFO @ Thu, 03 May 2018 23:06:25: #2 Build Peak Model... INFO @ Thu, 03 May 2018 23:06:25: #2 looking for paired plus/minus strand peaks... INFO @ Thu, 03 May 2018 23:06:25: #2 number of paired peaks: 36 WARNING @ Thu, 03 May 2018 23:06:25: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Thu, 03 May 2018 23:06:25: Process for pairing-model is terminated! cat: SRX3063092.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX3063092.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3063092.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX3063092.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。