Job ID = 11296266 sra ファイルのダウンロード中... Completed: 381614K bytes transferred in 14 seconds (219865K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 14494278 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969202/SRR5770189.sra Written 14494278 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969202/SRR5770189.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:46 14494278 reads; of these: 14494278 (100.00%) were unpaired; of these: 381286 (2.63%) aligned 0 times 12598257 (86.92%) aligned exactly 1 time 1514735 (10.45%) aligned >1 times 97.37% overall alignment rate Time searching: 00:02:46 Overall time: 00:02:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4108393 / 14112992 = 0.2911 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 05 Nov 2018 17:58:31: # Command line: callpeak -t SRX2969202.bam -f BAM -g 12100000 -n SRX2969202.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2969202.10 # format = BAM # ChIP-seq file = ['SRX2969202.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:58:31: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:58:31: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:58:31: # Command line: callpeak -t SRX2969202.bam -f BAM -g 12100000 -n SRX2969202.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2969202.20 # format = BAM # ChIP-seq file = ['SRX2969202.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:58:31: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:58:31: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:58:31: # Command line: callpeak -t SRX2969202.bam -f BAM -g 12100000 -n SRX2969202.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2969202.05 # format = BAM # ChIP-seq file = ['SRX2969202.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:58:31: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:58:31: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:58:37: 1000000 INFO @ Mon, 05 Nov 2018 17:58:38: 1000000 INFO @ Mon, 05 Nov 2018 17:58:38: 1000000 INFO @ Mon, 05 Nov 2018 17:58:43: 2000000 INFO @ Mon, 05 Nov 2018 17:58:45: 2000000 INFO @ Mon, 05 Nov 2018 17:58:45: 2000000 INFO @ Mon, 05 Nov 2018 17:58:50: 3000000 INFO @ Mon, 05 Nov 2018 17:58:51: 3000000 INFO @ Mon, 05 Nov 2018 17:58:51: 3000000 INFO @ Mon, 05 Nov 2018 17:58:56: 4000000 INFO @ Mon, 05 Nov 2018 17:58:58: 4000000 INFO @ Mon, 05 Nov 2018 17:58:58: 4000000 INFO @ Mon, 05 Nov 2018 17:59:02: 5000000 INFO @ Mon, 05 Nov 2018 17:59:05: 5000000 INFO @ Mon, 05 Nov 2018 17:59:05: 5000000 INFO @ Mon, 05 Nov 2018 17:59:09: 6000000 INFO @ Mon, 05 Nov 2018 17:59:12: 6000000 INFO @ Mon, 05 Nov 2018 17:59:12: 6000000 INFO @ Mon, 05 Nov 2018 17:59:15: 7000000 INFO @ Mon, 05 Nov 2018 17:59:19: 7000000 INFO @ Mon, 05 Nov 2018 17:59:19: 7000000 INFO @ Mon, 05 Nov 2018 17:59:21: 8000000 INFO @ Mon, 05 Nov 2018 17:59:26: 8000000 INFO @ Mon, 05 Nov 2018 17:59:26: 8000000 INFO @ Mon, 05 Nov 2018 17:59:28: 9000000 INFO @ Mon, 05 Nov 2018 17:59:33: 9000000 INFO @ Mon, 05 Nov 2018 17:59:33: 9000000 INFO @ Mon, 05 Nov 2018 17:59:34: 10000000 INFO @ Mon, 05 Nov 2018 17:59:34: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:59:34: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:59:34: #1 total tags in treatment: 10004599 INFO @ Mon, 05 Nov 2018 17:59:34: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:59:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:59:34: #1 tags after filtering in treatment: 10004599 INFO @ Mon, 05 Nov 2018 17:59:34: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:59:34: #1 finished! INFO @ Mon, 05 Nov 2018 17:59:34: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:59:34: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:59:35: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:59:35: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:59:35: Process for pairing-model is terminated! cat: SRX2969202.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969202.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969202.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969202.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 17:59:40: 10000000 INFO @ Mon, 05 Nov 2018 17:59:40: 10000000 INFO @ Mon, 05 Nov 2018 17:59:40: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:59:40: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:59:40: #1 total tags in treatment: 10004599 INFO @ Mon, 05 Nov 2018 17:59:40: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:59:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:59:40: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:59:40: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:59:40: #1 total tags in treatment: 10004599 INFO @ Mon, 05 Nov 2018 17:59:40: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:59:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:59:40: #1 tags after filtering in treatment: 10004599 INFO @ Mon, 05 Nov 2018 17:59:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:59:40: #1 finished! INFO @ Mon, 05 Nov 2018 17:59:40: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:59:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:59:40: #1 tags after filtering in treatment: 10004599 INFO @ Mon, 05 Nov 2018 17:59:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:59:40: #1 finished! INFO @ Mon, 05 Nov 2018 17:59:40: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:59:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:59:41: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:59:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:59:41: Process for pairing-model is terminated! INFO @ Mon, 05 Nov 2018 17:59:41: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:59:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:59:41: Process for pairing-model is terminated! cat: SRX2969202.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません cat: SRX2969202.10_peaks.narrowPeakpass1 - making usageList (0 chroms): 1 millis : そのようなファイルやディレクトリはありません needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969202.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969202.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969202.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969202.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969202.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969202.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。