Job ID = 11296257 sra ファイルのダウンロード中... Completed: 399640K bytes transferred in 12 seconds (257620K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 14932034 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969195/SRR5770182.sra Written 14932034 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969195/SRR5770182.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:49 14932034 reads; of these: 14932034 (100.00%) were unpaired; of these: 334795 (2.24%) aligned 0 times 12603639 (84.41%) aligned exactly 1 time 1993600 (13.35%) aligned >1 times 97.76% overall alignment rate Time searching: 00:02:49 Overall time: 00:02:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4559129 / 14597239 = 0.3123 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 05 Nov 2018 17:56:08: # Command line: callpeak -t SRX2969195.bam -f BAM -g 12100000 -n SRX2969195.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2969195.10 # format = BAM # ChIP-seq file = ['SRX2969195.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:56:08: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:56:08: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:56:08: # Command line: callpeak -t SRX2969195.bam -f BAM -g 12100000 -n SRX2969195.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2969195.05 # format = BAM # ChIP-seq file = ['SRX2969195.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:56:08: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:56:08: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:56:08: # Command line: callpeak -t SRX2969195.bam -f BAM -g 12100000 -n SRX2969195.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2969195.20 # format = BAM # ChIP-seq file = ['SRX2969195.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:56:08: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:56:08: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:56:15: 1000000 INFO @ Mon, 05 Nov 2018 17:56:15: 1000000 INFO @ Mon, 05 Nov 2018 17:56:16: 1000000 INFO @ Mon, 05 Nov 2018 17:56:23: 2000000 INFO @ Mon, 05 Nov 2018 17:56:23: 2000000 INFO @ Mon, 05 Nov 2018 17:56:24: 2000000 INFO @ Mon, 05 Nov 2018 17:56:30: 3000000 INFO @ Mon, 05 Nov 2018 17:56:30: 3000000 INFO @ Mon, 05 Nov 2018 17:56:32: 3000000 INFO @ Mon, 05 Nov 2018 17:56:38: 4000000 INFO @ Mon, 05 Nov 2018 17:56:38: 4000000 INFO @ Mon, 05 Nov 2018 17:56:41: 4000000 INFO @ Mon, 05 Nov 2018 17:56:45: 5000000 INFO @ Mon, 05 Nov 2018 17:56:46: 5000000 INFO @ Mon, 05 Nov 2018 17:56:49: 5000000 INFO @ Mon, 05 Nov 2018 17:56:53: 6000000 INFO @ Mon, 05 Nov 2018 17:56:53: 6000000 INFO @ Mon, 05 Nov 2018 17:56:57: 6000000 INFO @ Mon, 05 Nov 2018 17:57:00: 7000000 INFO @ Mon, 05 Nov 2018 17:57:01: 7000000 INFO @ Mon, 05 Nov 2018 17:57:05: 7000000 INFO @ Mon, 05 Nov 2018 17:57:08: 8000000 INFO @ Mon, 05 Nov 2018 17:57:08: 8000000 INFO @ Mon, 05 Nov 2018 17:57:13: 8000000 INFO @ Mon, 05 Nov 2018 17:57:15: 9000000 INFO @ Mon, 05 Nov 2018 17:57:16: 9000000 INFO @ Mon, 05 Nov 2018 17:57:22: 9000000 INFO @ Mon, 05 Nov 2018 17:57:23: 10000000 INFO @ Mon, 05 Nov 2018 17:57:23: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:57:23: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:57:23: #1 total tags in treatment: 10038110 INFO @ Mon, 05 Nov 2018 17:57:23: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:57:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:57:23: #1 tags after filtering in treatment: 10038110 INFO @ Mon, 05 Nov 2018 17:57:23: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:57:23: #1 finished! INFO @ Mon, 05 Nov 2018 17:57:23: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:57:23: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:57:23: 10000000 INFO @ Mon, 05 Nov 2018 17:57:24: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:57:24: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:57:24: Process for pairing-model is terminated! cat: SRX2969195.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Mon, 05 Nov 2018 17:57:24: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:57:24: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:57:24: #1 total tags in treatment: 10038110 INFO @ Mon, 05 Nov 2018 17:57:24: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:57:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969195.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969195.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969195.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 17:57:24: #1 tags after filtering in treatment: 10038110 INFO @ Mon, 05 Nov 2018 17:57:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:57:24: #1 finished! INFO @ Mon, 05 Nov 2018 17:57:24: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:57:24: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:57:25: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:57:25: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:57:25: Process for pairing-model is terminated! cat: SRX2969195.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969195.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969195.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969195.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 17:57:29: 10000000 INFO @ Mon, 05 Nov 2018 17:57:29: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:57:29: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:57:29: #1 total tags in treatment: 10038110 INFO @ Mon, 05 Nov 2018 17:57:29: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:57:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:57:29: #1 tags after filtering in treatment: 10038110 INFO @ Mon, 05 Nov 2018 17:57:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:57:29: #1 finished! INFO @ Mon, 05 Nov 2018 17:57:29: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:57:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:57:30: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:57:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:57:30: Process for pairing-model is terminated! cat: SRX2969195.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969195.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969195.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969195.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。