Job ID = 11296255 sra ファイルのダウンロード中... Completed: 520685K bytes transferred in 17 seconds (248242K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 18654087 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969193/SRR5770180.sra Written 18654087 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969193/SRR5770180.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:36 18654087 reads; of these: 18654087 (100.00%) were unpaired; of these: 438033 (2.35%) aligned 0 times 15917423 (85.33%) aligned exactly 1 time 2298631 (12.32%) aligned >1 times 97.65% overall alignment rate Time searching: 00:03:36 Overall time: 00:03:36 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7304701 / 18216054 = 0.4010 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 05 Nov 2018 17:57:33: # Command line: callpeak -t SRX2969193.bam -f BAM -g 12100000 -n SRX2969193.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2969193.05 # format = BAM # ChIP-seq file = ['SRX2969193.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:57:33: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:57:33: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:57:33: # Command line: callpeak -t SRX2969193.bam -f BAM -g 12100000 -n SRX2969193.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2969193.20 # format = BAM # ChIP-seq file = ['SRX2969193.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:57:33: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:57:33: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:57:33: # Command line: callpeak -t SRX2969193.bam -f BAM -g 12100000 -n SRX2969193.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2969193.10 # format = BAM # ChIP-seq file = ['SRX2969193.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:57:33: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:57:33: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:57:41: 1000000 INFO @ Mon, 05 Nov 2018 17:57:41: 1000000 INFO @ Mon, 05 Nov 2018 17:57:41: 1000000 INFO @ Mon, 05 Nov 2018 17:57:48: 2000000 INFO @ Mon, 05 Nov 2018 17:57:48: 2000000 INFO @ Mon, 05 Nov 2018 17:57:49: 2000000 INFO @ Mon, 05 Nov 2018 17:57:56: 3000000 INFO @ Mon, 05 Nov 2018 17:57:56: 3000000 INFO @ Mon, 05 Nov 2018 17:57:58: 3000000 INFO @ Mon, 05 Nov 2018 17:58:03: 4000000 INFO @ Mon, 05 Nov 2018 17:58:04: 4000000 INFO @ Mon, 05 Nov 2018 17:58:06: 4000000 INFO @ Mon, 05 Nov 2018 17:58:10: 5000000 INFO @ Mon, 05 Nov 2018 17:58:11: 5000000 INFO @ Mon, 05 Nov 2018 17:58:14: 5000000 INFO @ Mon, 05 Nov 2018 17:58:18: 6000000 INFO @ Mon, 05 Nov 2018 17:58:19: 6000000 INFO @ Mon, 05 Nov 2018 17:58:22: 6000000 INFO @ Mon, 05 Nov 2018 17:58:25: 7000000 INFO @ Mon, 05 Nov 2018 17:58:26: 7000000 INFO @ Mon, 05 Nov 2018 17:58:30: 7000000 INFO @ Mon, 05 Nov 2018 17:58:32: 8000000 INFO @ Mon, 05 Nov 2018 17:58:34: 8000000 INFO @ Mon, 05 Nov 2018 17:58:38: 8000000 INFO @ Mon, 05 Nov 2018 17:58:39: 9000000 INFO @ Mon, 05 Nov 2018 17:58:42: 9000000 INFO @ Mon, 05 Nov 2018 17:58:47: 9000000 INFO @ Mon, 05 Nov 2018 17:58:47: 10000000 INFO @ Mon, 05 Nov 2018 17:58:49: 10000000 INFO @ Mon, 05 Nov 2018 17:58:54: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:58:54: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:58:54: #1 total tags in treatment: 10911353 INFO @ Mon, 05 Nov 2018 17:58:54: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:58:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:58:54: #1 tags after filtering in treatment: 10911353 INFO @ Mon, 05 Nov 2018 17:58:54: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:58:54: #1 finished! INFO @ Mon, 05 Nov 2018 17:58:54: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:58:54: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:58:55: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:58:55: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:58:55: Process for pairing-model is terminated! cat: SRX2969193.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969193.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969193.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969193.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 17:58:55: 10000000 INFO @ Mon, 05 Nov 2018 17:58:56: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:58:56: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:58:56: #1 total tags in treatment: 10911353 INFO @ Mon, 05 Nov 2018 17:58:56: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:58:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:58:57: #1 tags after filtering in treatment: 10911353 INFO @ Mon, 05 Nov 2018 17:58:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:58:57: #1 finished! INFO @ Mon, 05 Nov 2018 17:58:57: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:58:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:58:57: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:58:57: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:58:57: Process for pairing-model is terminated! cat: SRX2969193.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969193.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969193.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969193.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 17:59:02: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:59:02: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:59:02: #1 total tags in treatment: 10911353 INFO @ Mon, 05 Nov 2018 17:59:02: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:59:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:59:03: #1 tags after filtering in treatment: 10911353 INFO @ Mon, 05 Nov 2018 17:59:03: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:59:03: #1 finished! INFO @ Mon, 05 Nov 2018 17:59:03: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:59:03: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:59:03: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:59:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:59:03: Process for pairing-model is terminated! cat: SRX2969193.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969193.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969193.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969193.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。