Job ID = 11296214 sra ファイルのダウンロード中... Completed: 393111K bytes transferred in 16 seconds (198983K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 14691198 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969176/SRR5770163.sra Written 14691198 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969176/SRR5770163.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:48 14691198 reads; of these: 14691198 (100.00%) were unpaired; of these: 279414 (1.90%) aligned 0 times 12609678 (85.83%) aligned exactly 1 time 1802106 (12.27%) aligned >1 times 98.10% overall alignment rate Time searching: 00:02:48 Overall time: 00:02:49 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4483017 / 14411784 = 0.3111 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 05 Nov 2018 17:47:46: # Command line: callpeak -t SRX2969176.bam -f BAM -g 12100000 -n SRX2969176.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2969176.05 # format = BAM # ChIP-seq file = ['SRX2969176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:47:46: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:47:46: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:47:46: # Command line: callpeak -t SRX2969176.bam -f BAM -g 12100000 -n SRX2969176.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2969176.10 # format = BAM # ChIP-seq file = ['SRX2969176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:47:46: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:47:46: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:47:46: # Command line: callpeak -t SRX2969176.bam -f BAM -g 12100000 -n SRX2969176.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2969176.20 # format = BAM # ChIP-seq file = ['SRX2969176.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:47:46: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:47:46: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:47:53: 1000000 INFO @ Mon, 05 Nov 2018 17:47:53: 1000000 INFO @ Mon, 05 Nov 2018 17:47:53: 1000000 INFO @ Mon, 05 Nov 2018 17:47:59: 2000000 INFO @ Mon, 05 Nov 2018 17:47:59: 2000000 INFO @ Mon, 05 Nov 2018 17:47:59: 2000000 INFO @ Mon, 05 Nov 2018 17:48:05: 3000000 INFO @ Mon, 05 Nov 2018 17:48:05: 3000000 INFO @ Mon, 05 Nov 2018 17:48:06: 3000000 INFO @ Mon, 05 Nov 2018 17:48:11: 4000000 INFO @ Mon, 05 Nov 2018 17:48:11: 4000000 INFO @ Mon, 05 Nov 2018 17:48:12: 4000000 INFO @ Mon, 05 Nov 2018 17:48:17: 5000000 INFO @ Mon, 05 Nov 2018 17:48:18: 5000000 INFO @ Mon, 05 Nov 2018 17:48:19: 5000000 INFO @ Mon, 05 Nov 2018 17:48:24: 6000000 INFO @ Mon, 05 Nov 2018 17:48:24: 6000000 INFO @ Mon, 05 Nov 2018 17:48:26: 6000000 INFO @ Mon, 05 Nov 2018 17:48:30: 7000000 INFO @ Mon, 05 Nov 2018 17:48:31: 7000000 INFO @ Mon, 05 Nov 2018 17:48:33: 7000000 INFO @ Mon, 05 Nov 2018 17:48:36: 8000000 INFO @ Mon, 05 Nov 2018 17:48:37: 8000000 INFO @ Mon, 05 Nov 2018 17:48:39: 8000000 INFO @ Mon, 05 Nov 2018 17:48:42: 9000000 INFO @ Mon, 05 Nov 2018 17:48:44: 9000000 INFO @ Mon, 05 Nov 2018 17:48:46: 9000000 INFO @ Mon, 05 Nov 2018 17:48:48: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:48:48: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:48:48: #1 total tags in treatment: 9928767 INFO @ Mon, 05 Nov 2018 17:48:48: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:48:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:48:48: #1 tags after filtering in treatment: 9928767 INFO @ Mon, 05 Nov 2018 17:48:48: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:48:48: #1 finished! INFO @ Mon, 05 Nov 2018 17:48:48: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:48:48: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:48:49: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:48:49: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:48:49: Process for pairing-model is terminated! cat: SRX2969176.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969176.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969176.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969176.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 17:48:50: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:48:50: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:48:50: #1 total tags in treatment: 9928767 INFO @ Mon, 05 Nov 2018 17:48:50: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:48:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:48:50: #1 tags after filtering in treatment: 9928767 INFO @ Mon, 05 Nov 2018 17:48:50: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:48:50: #1 finished! INFO @ Mon, 05 Nov 2018 17:48:50: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:48:50: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:48:51: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:48:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:48:51: Process for pairing-model is terminated! cat: SRX2969176.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969176.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969176.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969176.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 17:48:52: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:48:52: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:48:52: #1 total tags in treatment: 9928767 INFO @ Mon, 05 Nov 2018 17:48:52: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:48:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:48:52: #1 tags after filtering in treatment: 9928767 INFO @ Mon, 05 Nov 2018 17:48:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:48:52: #1 finished! INFO @ Mon, 05 Nov 2018 17:48:52: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:48:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:48:53: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:48:53: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:48:53: Process for pairing-model is terminated! cat: SRX2969176.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969176.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969176.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969176.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。