Job ID = 11296213 sra ファイルのダウンロード中... Completed: 368269K bytes transferred in 14 seconds (203465K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 14237772 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969175/SRR5770162.sra Written 14237772 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2969175/SRR5770162.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:39 14237772 reads; of these: 14237772 (100.00%) were unpaired; of these: 304093 (2.14%) aligned 0 times 12102326 (85.00%) aligned exactly 1 time 1831353 (12.86%) aligned >1 times 97.86% overall alignment rate Time searching: 00:02:39 Overall time: 00:02:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4772949 / 13933679 = 0.3425 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 05 Nov 2018 17:47:22: # Command line: callpeak -t SRX2969175.bam -f BAM -g 12100000 -n SRX2969175.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2969175.10 # format = BAM # ChIP-seq file = ['SRX2969175.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:47:22: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:47:22: # Command line: callpeak -t SRX2969175.bam -f BAM -g 12100000 -n SRX2969175.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2969175.05 # format = BAM # ChIP-seq file = ['SRX2969175.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:47:22: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:47:22: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:47:22: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:47:22: # Command line: callpeak -t SRX2969175.bam -f BAM -g 12100000 -n SRX2969175.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2969175.20 # format = BAM # ChIP-seq file = ['SRX2969175.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 05 Nov 2018 17:47:22: #1 read tag files... INFO @ Mon, 05 Nov 2018 17:47:22: #1 read treatment tags... INFO @ Mon, 05 Nov 2018 17:47:29: 1000000 INFO @ Mon, 05 Nov 2018 17:47:30: 1000000 INFO @ Mon, 05 Nov 2018 17:47:30: 1000000 INFO @ Mon, 05 Nov 2018 17:47:36: 2000000 INFO @ Mon, 05 Nov 2018 17:47:39: 2000000 INFO @ Mon, 05 Nov 2018 17:47:39: 2000000 INFO @ Mon, 05 Nov 2018 17:47:44: 3000000 INFO @ Mon, 05 Nov 2018 17:47:48: 3000000 INFO @ Mon, 05 Nov 2018 17:47:49: 3000000 INFO @ Mon, 05 Nov 2018 17:47:51: 4000000 INFO @ Mon, 05 Nov 2018 17:47:57: 4000000 INFO @ Mon, 05 Nov 2018 17:47:57: 4000000 INFO @ Mon, 05 Nov 2018 17:47:59: 5000000 INFO @ Mon, 05 Nov 2018 17:48:06: 6000000 INFO @ Mon, 05 Nov 2018 17:48:06: 5000000 INFO @ Mon, 05 Nov 2018 17:48:06: 5000000 INFO @ Mon, 05 Nov 2018 17:48:14: 7000000 INFO @ Mon, 05 Nov 2018 17:48:15: 6000000 INFO @ Mon, 05 Nov 2018 17:48:15: 6000000 INFO @ Mon, 05 Nov 2018 17:48:21: 8000000 INFO @ Mon, 05 Nov 2018 17:48:24: 7000000 INFO @ Mon, 05 Nov 2018 17:48:24: 7000000 INFO @ Mon, 05 Nov 2018 17:48:29: 9000000 INFO @ Mon, 05 Nov 2018 17:48:30: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:48:30: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:48:30: #1 total tags in treatment: 9160730 INFO @ Mon, 05 Nov 2018 17:48:30: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:48:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:48:30: #1 tags after filtering in treatment: 9160730 INFO @ Mon, 05 Nov 2018 17:48:30: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:48:30: #1 finished! INFO @ Mon, 05 Nov 2018 17:48:30: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:48:30: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:48:30: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:48:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:48:30: Process for pairing-model is terminated! cat: SRX2969175.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969175.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969175.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969175.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 17:48:33: 8000000 INFO @ Mon, 05 Nov 2018 17:48:33: 8000000 INFO @ Mon, 05 Nov 2018 17:48:42: 9000000 INFO @ Mon, 05 Nov 2018 17:48:42: 9000000 INFO @ Mon, 05 Nov 2018 17:48:43: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:48:43: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:48:43: #1 total tags in treatment: 9160730 INFO @ Mon, 05 Nov 2018 17:48:43: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:48:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:48:43: #1 tag size is determined as 50 bps INFO @ Mon, 05 Nov 2018 17:48:43: #1 tag size = 50 INFO @ Mon, 05 Nov 2018 17:48:43: #1 total tags in treatment: 9160730 INFO @ Mon, 05 Nov 2018 17:48:43: #1 user defined the maximum tags... INFO @ Mon, 05 Nov 2018 17:48:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 05 Nov 2018 17:48:43: #1 tags after filtering in treatment: 9160730 INFO @ Mon, 05 Nov 2018 17:48:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:48:43: #1 finished! INFO @ Mon, 05 Nov 2018 17:48:43: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:48:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:48:43: #1 tags after filtering in treatment: 9160730 INFO @ Mon, 05 Nov 2018 17:48:43: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 05 Nov 2018 17:48:43: #1 finished! INFO @ Mon, 05 Nov 2018 17:48:43: #2 Build Peak Model... INFO @ Mon, 05 Nov 2018 17:48:43: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 05 Nov 2018 17:48:43: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:48:43: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:48:43: Process for pairing-model is terminated! cat: SRX2969175.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969175.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969175.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969175.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 05 Nov 2018 17:48:44: #2 number of paired peaks: 0 WARNING @ Mon, 05 Nov 2018 17:48:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 05 Nov 2018 17:48:44: Process for pairing-model is terminated! cat: SRX2969175.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2969175.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969175.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2969175.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。