Job ID = 2010174 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,186,978 reads read : 16,186,978 reads written : 16,186,978 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:56 16186978 reads; of these: 16186978 (100.00%) were unpaired; of these: 469806 (2.90%) aligned 0 times 13725532 (84.79%) aligned exactly 1 time 1991640 (12.30%) aligned >1 times 97.10% overall alignment rate Time searching: 00:02:56 Overall time: 00:02:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6780844 / 15717172 = 0.4314 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:49:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:49:10: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:49:10: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:49:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:49:11: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:49:11: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:49:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:49:12: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:49:12: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:49:17: 1000000 INFO @ Fri, 05 Jul 2019 21:49:19: 1000000 INFO @ Fri, 05 Jul 2019 21:49:20: 1000000 INFO @ Fri, 05 Jul 2019 21:49:24: 2000000 INFO @ Fri, 05 Jul 2019 21:49:26: 2000000 INFO @ Fri, 05 Jul 2019 21:49:27: 2000000 INFO @ Fri, 05 Jul 2019 21:49:31: 3000000 INFO @ Fri, 05 Jul 2019 21:49:34: 3000000 INFO @ Fri, 05 Jul 2019 21:49:35: 3000000 INFO @ Fri, 05 Jul 2019 21:49:37: 4000000 INFO @ Fri, 05 Jul 2019 21:49:41: 4000000 INFO @ Fri, 05 Jul 2019 21:49:42: 4000000 INFO @ Fri, 05 Jul 2019 21:49:43: 5000000 INFO @ Fri, 05 Jul 2019 21:49:48: 5000000 INFO @ Fri, 05 Jul 2019 21:49:49: 5000000 INFO @ Fri, 05 Jul 2019 21:49:50: 6000000 INFO @ Fri, 05 Jul 2019 21:49:54: 6000000 INFO @ Fri, 05 Jul 2019 21:49:56: 6000000 INFO @ Fri, 05 Jul 2019 21:49:56: 7000000 INFO @ Fri, 05 Jul 2019 21:50:01: 7000000 INFO @ Fri, 05 Jul 2019 21:50:03: 7000000 INFO @ Fri, 05 Jul 2019 21:50:03: 8000000 INFO @ Fri, 05 Jul 2019 21:50:08: 8000000 INFO @ Fri, 05 Jul 2019 21:50:09: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:50:09: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:50:09: #1 total tags in treatment: 8936328 INFO @ Fri, 05 Jul 2019 21:50:09: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:50:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:50:09: #1 tags after filtering in treatment: 8936328 INFO @ Fri, 05 Jul 2019 21:50:09: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:50:09: #1 finished! INFO @ Fri, 05 Jul 2019 21:50:09: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:50:09: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:50:09: 8000000 INFO @ Fri, 05 Jul 2019 21:50:09: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:50:09: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:50:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:50:15: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:50:15: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:50:15: #1 total tags in treatment: 8936328 INFO @ Fri, 05 Jul 2019 21:50:15: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:50:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:50:15: #1 tags after filtering in treatment: 8936328 INFO @ Fri, 05 Jul 2019 21:50:15: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:50:15: #1 finished! INFO @ Fri, 05 Jul 2019 21:50:15: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:50:15: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:50:16: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:50:16: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:50:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:50:16: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:50:16: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:50:16: #1 total tags in treatment: 8936328 INFO @ Fri, 05 Jul 2019 21:50:16: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:50:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:50:16: #1 tags after filtering in treatment: 8936328 INFO @ Fri, 05 Jul 2019 21:50:16: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:50:16: #1 finished! INFO @ Fri, 05 Jul 2019 21:50:16: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:50:16: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:50:17: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:50:17: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:50:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894842/SRX2894842.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。