Job ID = 2010169 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2019-07-05T12:41:36 fasterq-dump.2.9.6 sys: timeout exhausted while reading file within network system module - mbedtls_ssl_read returned -76 ( NET - Reading information from the socket failed ) spots read : 17,007,038 reads read : 17,007,038 reads written : 17,007,038 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:15 17007038 reads; of these: 17007038 (100.00%) were unpaired; of these: 322178 (1.89%) aligned 0 times 14509750 (85.32%) aligned exactly 1 time 2175110 (12.79%) aligned >1 times 98.11% overall alignment rate Time searching: 00:04:15 Overall time: 00:04:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 7931997 / 16684860 = 0.4754 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:53:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:53:31: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:53:31: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:53:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:53:32: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:53:32: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:53:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:53:33: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:53:33: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:53:39: 1000000 INFO @ Fri, 05 Jul 2019 21:53:39: 1000000 INFO @ Fri, 05 Jul 2019 21:53:41: 1000000 INFO @ Fri, 05 Jul 2019 21:53:47: 2000000 INFO @ Fri, 05 Jul 2019 21:53:47: 2000000 INFO @ Fri, 05 Jul 2019 21:53:49: 2000000 INFO @ Fri, 05 Jul 2019 21:53:54: 3000000 INFO @ Fri, 05 Jul 2019 21:53:55: 3000000 INFO @ Fri, 05 Jul 2019 21:53:56: 3000000 INFO @ Fri, 05 Jul 2019 21:54:01: 4000000 INFO @ Fri, 05 Jul 2019 21:54:02: 4000000 INFO @ Fri, 05 Jul 2019 21:54:03: 4000000 INFO @ Fri, 05 Jul 2019 21:54:08: 5000000 INFO @ Fri, 05 Jul 2019 21:54:10: 5000000 INFO @ Fri, 05 Jul 2019 21:54:10: 5000000 INFO @ Fri, 05 Jul 2019 21:54:14: 6000000 INFO @ Fri, 05 Jul 2019 21:54:17: 6000000 INFO @ Fri, 05 Jul 2019 21:54:17: 6000000 INFO @ Fri, 05 Jul 2019 21:54:21: 7000000 INFO @ Fri, 05 Jul 2019 21:54:23: 7000000 INFO @ Fri, 05 Jul 2019 21:54:25: 7000000 INFO @ Fri, 05 Jul 2019 21:54:28: 8000000 INFO @ Fri, 05 Jul 2019 21:54:30: 8000000 INFO @ Fri, 05 Jul 2019 21:54:32: 8000000 INFO @ Fri, 05 Jul 2019 21:54:33: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:54:33: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:54:33: #1 total tags in treatment: 8752863 INFO @ Fri, 05 Jul 2019 21:54:33: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:54:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:54:33: #1 tags after filtering in treatment: 8752863 INFO @ Fri, 05 Jul 2019 21:54:33: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:54:33: #1 finished! INFO @ Fri, 05 Jul 2019 21:54:33: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:54:33: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:54:34: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:54:34: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:54:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:54:35: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:54:35: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:54:35: #1 total tags in treatment: 8752863 INFO @ Fri, 05 Jul 2019 21:54:35: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:54:35: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:54:36: #1 tags after filtering in treatment: 8752863 INFO @ Fri, 05 Jul 2019 21:54:36: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:54:36: #1 finished! INFO @ Fri, 05 Jul 2019 21:54:36: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:54:36: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:54:36: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:54:36: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:54:36: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:54:38: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:54:38: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:54:38: #1 total tags in treatment: 8752863 INFO @ Fri, 05 Jul 2019 21:54:38: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:54:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:54:38: #1 tags after filtering in treatment: 8752863 INFO @ Fri, 05 Jul 2019 21:54:38: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:54:38: #1 finished! INFO @ Fri, 05 Jul 2019 21:54:38: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:54:38: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:54:38: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:54:38: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:54:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894838/SRX2894838.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。