Job ID = 2010154 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,655,786 reads read : 20,655,786 reads written : 20,655,786 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:03:39 20655786 reads; of these: 20655786 (100.00%) were unpaired; of these: 485286 (2.35%) aligned 0 times 16838032 (81.52%) aligned exactly 1 time 3332468 (16.13%) aligned >1 times 97.65% overall alignment rate Time searching: 00:03:39 Overall time: 00:03:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 8313703 / 20170500 = 0.4122 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:47:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:47:51: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:47:51: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:47:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:47:52: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:47:52: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:47:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:47:53: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:47:53: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:47:59: 1000000 INFO @ Fri, 05 Jul 2019 21:48:00: 1000000 INFO @ Fri, 05 Jul 2019 21:48:02: 1000000 INFO @ Fri, 05 Jul 2019 21:48:07: 2000000 INFO @ Fri, 05 Jul 2019 21:48:09: 2000000 INFO @ Fri, 05 Jul 2019 21:48:10: 2000000 INFO @ Fri, 05 Jul 2019 21:48:16: 3000000 INFO @ Fri, 05 Jul 2019 21:48:17: 3000000 INFO @ Fri, 05 Jul 2019 21:48:18: 3000000 INFO @ Fri, 05 Jul 2019 21:48:24: 4000000 INFO @ Fri, 05 Jul 2019 21:48:26: 4000000 INFO @ Fri, 05 Jul 2019 21:48:27: 4000000 INFO @ Fri, 05 Jul 2019 21:48:32: 5000000 INFO @ Fri, 05 Jul 2019 21:48:34: 5000000 INFO @ Fri, 05 Jul 2019 21:48:35: 5000000 INFO @ Fri, 05 Jul 2019 21:48:40: 6000000 INFO @ Fri, 05 Jul 2019 21:48:42: 6000000 INFO @ Fri, 05 Jul 2019 21:48:43: 6000000 INFO @ Fri, 05 Jul 2019 21:48:48: 7000000 INFO @ Fri, 05 Jul 2019 21:48:51: 7000000 INFO @ Fri, 05 Jul 2019 21:48:52: 7000000 INFO @ Fri, 05 Jul 2019 21:48:56: 8000000 INFO @ Fri, 05 Jul 2019 21:48:59: 8000000 INFO @ Fri, 05 Jul 2019 21:49:00: 8000000 INFO @ Fri, 05 Jul 2019 21:49:04: 9000000 INFO @ Fri, 05 Jul 2019 21:49:07: 9000000 INFO @ Fri, 05 Jul 2019 21:49:09: 9000000 INFO @ Fri, 05 Jul 2019 21:49:12: 10000000 INFO @ Fri, 05 Jul 2019 21:49:16: 10000000 INFO @ Fri, 05 Jul 2019 21:49:17: 10000000 INFO @ Fri, 05 Jul 2019 21:49:20: 11000000 INFO @ Fri, 05 Jul 2019 21:49:24: 11000000 INFO @ Fri, 05 Jul 2019 21:49:25: 11000000 INFO @ Fri, 05 Jul 2019 21:49:27: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:49:27: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:49:27: #1 total tags in treatment: 11856797 INFO @ Fri, 05 Jul 2019 21:49:27: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:49:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:49:27: #1 tags after filtering in treatment: 11856797 INFO @ Fri, 05 Jul 2019 21:49:27: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:49:27: #1 finished! INFO @ Fri, 05 Jul 2019 21:49:27: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:49:27: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:49:28: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:49:28: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:49:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:49:31: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:49:31: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:49:31: #1 total tags in treatment: 11856797 INFO @ Fri, 05 Jul 2019 21:49:31: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:49:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:49:31: #1 tags after filtering in treatment: 11856797 INFO @ Fri, 05 Jul 2019 21:49:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:49:31: #1 finished! INFO @ Fri, 05 Jul 2019 21:49:31: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:49:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:49:32: #1 tag size is determined as 51 bps INFO @ Fri, 05 Jul 2019 21:49:32: #1 tag size = 51 INFO @ Fri, 05 Jul 2019 21:49:32: #1 total tags in treatment: 11856797 INFO @ Fri, 05 Jul 2019 21:49:32: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:49:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:49:32: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:49:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:49:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.10_peaks.narrowPeak: No such file or directory INFO @ Fri, 05 Jul 2019 21:49:32: #1 tags after filtering in treatment: 11856797 INFO @ Fri, 05 Jul 2019 21:49:32: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:49:32: #1 finished! INFO @ Fri, 05 Jul 2019 21:49:32: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:49:32: #2 looking for paired plus/minus strand peaks... pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:49:33: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:49:33: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:49:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2894824/SRX2894824.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。