Job ID = 12531516 SRX = SRX2797025 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11113027 spots for SRR5527839/SRR5527839.sra Written 11113027 spots for SRR5527839/SRR5527839.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Warning: skipping mate #1 of read 'SRR5527839.37095 37095 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.37095 37095 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.86337 86337 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.86337 86337 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.151776 151776 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.151776 151776 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.162721 162721 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.162721 162721 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.165004 165004 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.165004 165004 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.169282 169282 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.169282 169282 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.193408 193408 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.193408 193408 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.213961 213961 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.213961 213961 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.231441 231441 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.231441 231441 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.248502 248502 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.248502 248502 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.284441 284441 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.284441 284441 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.290465 290465 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.290465 290465 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.333338 333338 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.333338 333338 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.335688 335688 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.335688 335688 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.353019 353019 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.353019 353019 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.365988 365988 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.365988 365988 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.369879 369879 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.369879 369879 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.418927 418927 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.418927 418927 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.426669 426669 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.426669 426669 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.426669 426669 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.426669 426669 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.445842 445842 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.445842 445842 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.579471 579471 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.579471 579471 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.639205 639205 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.639205 639205 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.646694 646694 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.646694 646694 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.665630 665630 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.665630 665630 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.671753 671753 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.671753 671753 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.714765 714765 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.714765 714765 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.752238 752238 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.752238 752238 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.752849 752849 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.752849 752849 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.770653 770653 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.770653 770653 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.779069 779069 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.779069 779069 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.854876 854876 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.854876 854876 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.882664 882664 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.882664 882664 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.895123 895123 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.895123 895123 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.925010 925010 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.925010 925010 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.926730 926730 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.926730 926730 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.927176 927176 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.927176 927176 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.971219 971219 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.971219 971219 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.979703 979703 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.979703 979703 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1016473 1016473 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1016473 1016473 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1088593 1088593 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1088593 1088593 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1092941 1092941 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1092941 1092941 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1130760 1130760 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1130760 1130760 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1172780 1172780 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1172780 1172780 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1190202 1190202 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1190202 1190202 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1332742 1332742 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1332742 1332742 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1389868 1389868 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1389868 1389868 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1424071 1424071 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1424071 1424071 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1427095 1427095 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1427095 1427095 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1436956 1436956 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1436956 1436956 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1450272 1450272 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1450272 1450272 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1476256 1476256 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1476256 1476256 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1480524 1480524 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1480524 1480524 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1490896 1490896 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1490896 1490896 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1498634 1498634 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1498634 1498634 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1542382 1542382 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1542382 1542382 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1555432 1555432 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1555432 1555432 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1562167 1562167 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1562167 1562167 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1590041 1590041 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1590041 1590041 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1633061 1633061 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1633061 1633061 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1675798 1675798 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1675798 1675798 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1678698 1678698 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1678698 1678698 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1682529 1682529 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1682529 1682529 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1682997 1682997 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1682997 1682997 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1759741 1759741 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1759741 1759741 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1894947 1894947 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1894947 1894947 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.1900074 1900074 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.1900074 1900074 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.1985551 1985551 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.1985551 1985551 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2002101 2002101 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2002101 2002101 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2004157 2004157 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2004157 2004157 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2057971 2057971 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2057971 2057971 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.2113085 2113085 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.2113085 2113085 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2133613 2133613 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2133613 2133613 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.2139583 2139583 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.2139583 2139583 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2156877 2156877 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2156877 2156877 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2179184 2179184 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2179184 2179184 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2298646 2298646 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2298646 2298646 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2333623 2333623 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2333623 2333623 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2406691 2406691 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2406691 2406691 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.2419074 2419074 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.2419074 2419074 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2424614 2424614 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2424614 2424614 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.2553768 2553768 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.2553768 2553768 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.2680085 2680085 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.2680085 2680085 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2791465 2791465 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2791465 2791465 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2847039 2847039 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2847039 2847039 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.2847172 2847172 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.2847172 2847172 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.2869653 2869653 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.2869653 2869653 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2870957 2870957 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2870957 2870957 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.2962502 2962502 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.2962502 2962502 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.2965231 2965231 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.2965231 2965231 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3061933 3061933 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3061933 3061933 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3123645 3123645 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3123645 3123645 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3153255 3153255 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3153255 3153255 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3185758 3185758 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3185758 3185758 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3214934 3214934 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3214934 3214934 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3215057 3215057 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3215057 3215057 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3222555 3222555 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3222555 3222555 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3236421 3236421 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3236421 3236421 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3260845 3260845 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3260845 3260845 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3271813 3271813 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3271813 3271813 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3286224 3286224 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3286224 3286224 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3288127 3288127 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3288127 3288127 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3351425 3351425 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3351425 3351425 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3352852 3352852 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3352852 3352852 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3356260 3356260 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3356260 3356260 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3373370 3373370 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3373370 3373370 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3382956 3382956 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3382956 3382956 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3414751 3414751 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3414751 3414751 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3428705 3428705 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3428705 3428705 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3483149 3483149 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3483149 3483149 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3496118 3496118 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3496118 3496118 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3496831 3496831 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3496831 3496831 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3521916 3521916 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3521916 3521916 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3569170 3569170 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3569170 3569170 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3569170 3569170 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3569170 3569170 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3577679 3577679 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3577679 3577679 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3636083 3636083 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3636083 3636083 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3665951 3665951 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3665951 3665951 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3673981 3673981 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3673981 3673981 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3749010 3749010 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3749010 3749010 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3813758 3813758 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3813758 3813758 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3822750 3822750 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3822750 3822750 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3833332 3833332 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3833332 3833332 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3844261 3844261 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3844261 3844261 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3896645 3896645 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3896645 3896645 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.3912888 3912888 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.3912888 3912888 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.3940354 3940354 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.3940354 3940354 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4022400 4022400 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4022400 4022400 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4022400 4022400 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4022400 4022400 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4031976 4031976 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4031976 4031976 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4072591 4072591 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4072591 4072591 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4266380 4266380 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4266380 4266380 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4270439 4270439 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4270439 4270439 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4358451 4358451 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4358451 4358451 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4391743 4391743 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4391743 4391743 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4414658 4414658 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4414658 4414658 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4445256 4445256 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4445256 4445256 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4481962 4481962 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4481962 4481962 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4519184 4519184 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4519184 4519184 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4555973 4555973 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4555973 4555973 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4614076 4614076 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4614076 4614076 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4634995 4634995 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4634995 4634995 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4676421 4676421 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4676421 4676421 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4707762 4707762 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4707762 4707762 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4808999 4808999 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4808999 4808999 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4815606 4815606 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4815606 4815606 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.4840276 4840276 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.4840276 4840276 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4901464 4901464 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4901464 4901464 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4931100 4931100 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4931100 4931100 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4948996 4948996 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4948996 4948996 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4958304 4958304 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4958304 4958304 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.4995981 4995981 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.4995981 4995981 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.5042186 5042186 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.5042186 5042186 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.5165720 5165720 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.5165720 5165720 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.5198525 5198525 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.5198525 5198525 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.5249042 5249042 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.5249042 5249042 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.5265296 5265296 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.5265296 5265296 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.5312606 5312606 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.5312606 5312606 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.5333781 5333781 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.5333781 5333781 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.5497793 5497793 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.5497793 5497793 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.5744039 5744039 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.5744039 5744039 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.5754453 5754453 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.5754453 5754453 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.5790164 5790164 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.5790164 5790164 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.5899668 5899668 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.5899668 5899668 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.5924500 5924500 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.5924500 5924500 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.5929222 5929222 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.5929222 5929222 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.5970653 5970653 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.5970653 5970653 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.5987900 5987900 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.5987900 5987900 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6006265 6006265 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6006265 6006265 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6068781 6068781 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6068781 6068781 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6129041 6129041 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6129041 6129041 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.6221684 6221684 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.6221684 6221684 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.6314780 6314780 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.6314780 6314780 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6319794 6319794 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6319794 6319794 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.6371182 6371182 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.6371182 6371182 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6395187 6395187 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6395187 6395187 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6405928 6405928 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6405928 6405928 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.6411747 6411747 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.6411747 6411747 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6515215 6515215 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6515215 6515215 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6530898 6530898 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6530898 6530898 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6537801 6537801 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6537801 6537801 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.6538352 6538352 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.6538352 6538352 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6565024 6565024 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6565024 6565024 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6593155 6593155 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6593155 6593155 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6657698 6657698 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6657698 6657698 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6660798 6660798 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6660798 6660798 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.6769628 6769628 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.6769628 6769628 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.6810555 6810555 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.6810555 6810555 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.6878630 6878630 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.6878630 6878630 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.6932007 6932007 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.6932007 6932007 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7017914 7017914 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7017914 7017914 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7033541 7033541 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7033541 7033541 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7059946 7059946 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7059946 7059946 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7141808 7141808 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7141808 7141808 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.7201198 7201198 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.7201198 7201198 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7208896 7208896 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7208896 7208896 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7224236 7224236 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7224236 7224236 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.7256624 7256624 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.7256624 7256624 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7283336 7283336 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7283336 7283336 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.7289014 7289014 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.7289014 7289014 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7383632 7383632 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7383632 7383632 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7427150 7427150 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7427150 7427150 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.7430065 7430065 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.7430065 7430065 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7496381 7496381 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7496381 7496381 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7497165 7497165 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7497165 7497165 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7549710 7549710 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7549710 7549710 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7571692 7571692 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7571692 7571692 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7648107 7648107 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7648107 7648107 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.7658516 7658516 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.7658516 7658516 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.7704722 7704722 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.7704722 7704722 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7720662 7720662 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7720662 7720662 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7825163 7825163 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7825163 7825163 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7880439 7880439 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7880439 7880439 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.7953294 7953294 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.7953294 7953294 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.7957596 7957596 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.7957596 7957596 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8002657 8002657 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8002657 8002657 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8051587 8051587 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8051587 8051587 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8052932 8052932 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8052932 8052932 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.8090410 8090410 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.8090410 8090410 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8351006 8351006 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8351006 8351006 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.8416241 8416241 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.8416241 8416241 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.8461171 8461171 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.8461171 8461171 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.8470593 8470593 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.8470593 8470593 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.8555207 8555207 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.8555207 8555207 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.8566892 8566892 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.8566892 8566892 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.8580422 8580422 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.8580422 8580422 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8594547 8594547 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8594547 8594547 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8655499 8655499 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8655499 8655499 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8725635 8725635 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8725635 8725635 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8833469 8833469 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8833469 8833469 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8855215 8855215 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8855215 8855215 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8879553 8879553 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8879553 8879553 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8891777 8891777 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8891777 8891777 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8900142 8900142 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8900142 8900142 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.8917038 8917038 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.8917038 8917038 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.8925089 8925089 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.8925089 8925089 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.9104647 9104647 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.9104647 9104647 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.9203218 9203218 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.9203218 9203218 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.9203218 9203218 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.9203218 9203218 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.9210512 9210512 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.9210512 9210512 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.9220506 9220506 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.9220506 9220506 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.9223721 9223721 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.9223721 9223721 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.9299436 9299436 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.9299436 9299436 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.9363693 9363693 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.9363693 9363693 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.9416235 9416235 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.9416235 9416235 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.9443982 9443982 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.9443982 9443982 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.9445221 9445221 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.9445221 9445221 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.9509328 9509328 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.9509328 9509328 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.9527396 9527396 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.9527396 9527396 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.9547164 9547164 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.9547164 9547164 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.9596501 9596501 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.9596501 9596501 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.9795246 9795246 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.9795246 9795246 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.9994588 9994588 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.9994588 9994588 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10018442 10018442 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10018442 10018442 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10028126 10028126 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10028126 10028126 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10075174 10075174 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10075174 10075174 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10134500 10134500 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10134500 10134500 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10179270 10179270 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10179270 10179270 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10185173 10185173 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10185173 10185173 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10272235 10272235 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10272235 10272235 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10340419 10340419 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10340419 10340419 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10348255 10348255 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10348255 10348255 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10379757 10379757 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10379757 10379757 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10403956 10403956 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10403956 10403956 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10489759 10489759 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10489759 10489759 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10538473 10538473 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10538473 10538473 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10559377 10559377 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10559377 10559377 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10627301 10627301 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10627301 10627301 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10651858 10651858 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10651858 10651858 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10651858 10651858 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10651858 10651858 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10665394 10665394 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10665394 10665394 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10802565 10802565 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10802565 10802565 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10867818 10867818 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10867818 10867818 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10893652 10893652 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10893652 10893652 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.10900052 10900052 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.10900052 10900052 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527839.10907657 10907657 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527839.10907657 10907657 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527839.11006995 11006995 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527839.11006995 11006995 length=1' because it was < 2 characters long Multiseed full-index search: 00:09:28 11113027 reads; of these: 11113027 (100.00%) were paired; of these: 1070579 (9.63%) aligned concordantly 0 times 5965235 (53.68%) aligned concordantly exactly 1 time 4077213 (36.69%) aligned concordantly >1 times ---- 1070579 pairs aligned concordantly 0 times; of these: 458840 (42.86%) aligned discordantly 1 time ---- 611739 pairs aligned 0 times concordantly or discordantly; of these: 1223478 mates make up the pairs; of these: 437642 (35.77%) aligned 0 times 87041 (7.11%) aligned exactly 1 time 698795 (57.12%) aligned >1 times 98.03% overall alignment rate Time searching: 00:09:28 Overall time: 00:09:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3525191 / 10441996 = 0.3376 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 08:15:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 08:15:34: #1 read tag files... INFO @ Sat, 17 Apr 2021 08:15:34: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 08:15:42: 1000000 INFO @ Sat, 17 Apr 2021 08:15:50: 2000000 INFO @ Sat, 17 Apr 2021 08:15:59: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 08:16:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 08:16:03: #1 read tag files... INFO @ Sat, 17 Apr 2021 08:16:03: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 08:16:08: 4000000 INFO @ Sat, 17 Apr 2021 08:16:12: 1000000 INFO @ Sat, 17 Apr 2021 08:16:16: 5000000 INFO @ Sat, 17 Apr 2021 08:16:20: 2000000 INFO @ Sat, 17 Apr 2021 08:16:25: 6000000 INFO @ Sat, 17 Apr 2021 08:16:29: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 08:16:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 08:16:33: #1 read tag files... INFO @ Sat, 17 Apr 2021 08:16:33: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 08:16:34: 7000000 INFO @ Sat, 17 Apr 2021 08:16:38: 4000000 INFO @ Sat, 17 Apr 2021 08:16:41: 1000000 INFO @ Sat, 17 Apr 2021 08:16:43: 8000000 INFO @ Sat, 17 Apr 2021 08:16:47: 5000000 INFO @ Sat, 17 Apr 2021 08:16:49: 2000000 INFO @ Sat, 17 Apr 2021 08:16:52: 9000000 INFO @ Sat, 17 Apr 2021 08:16:56: 3000000 INFO @ Sat, 17 Apr 2021 08:16:56: 6000000 INFO @ Sat, 17 Apr 2021 08:17:01: 10000000 INFO @ Sat, 17 Apr 2021 08:17:03: 4000000 INFO @ Sat, 17 Apr 2021 08:17:04: 7000000 INFO @ Sat, 17 Apr 2021 08:17:10: 11000000 INFO @ Sat, 17 Apr 2021 08:17:10: 5000000 INFO @ Sat, 17 Apr 2021 08:17:13: 8000000 INFO @ Sat, 17 Apr 2021 08:17:16: 6000000 INFO @ Sat, 17 Apr 2021 08:17:19: 12000000 INFO @ Sat, 17 Apr 2021 08:17:22: 9000000 INFO @ Sat, 17 Apr 2021 08:17:23: 7000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 17 Apr 2021 08:17:27: 13000000 INFO @ Sat, 17 Apr 2021 08:17:30: 8000000 INFO @ Sat, 17 Apr 2021 08:17:31: 10000000 BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 08:17:36: 14000000 INFO @ Sat, 17 Apr 2021 08:17:37: 9000000 INFO @ Sat, 17 Apr 2021 08:17:40: 11000000 INFO @ Sat, 17 Apr 2021 08:17:43: #1 tag size is determined as 51 bps INFO @ Sat, 17 Apr 2021 08:17:43: #1 tag size = 51 INFO @ Sat, 17 Apr 2021 08:17:43: #1 total tags in treatment: 6562181 INFO @ Sat, 17 Apr 2021 08:17:43: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 08:17:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 08:17:43: #1 tags after filtering in treatment: 3193603 INFO @ Sat, 17 Apr 2021 08:17:43: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 17 Apr 2021 08:17:43: #1 finished! INFO @ Sat, 17 Apr 2021 08:17:43: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 08:17:43: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 08:17:43: #2 number of paired peaks: 35 WARNING @ Sat, 17 Apr 2021 08:17:43: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 08:17:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.05_peaks.narrowPeak: No such file or directory INFO @ Sat, 17 Apr 2021 08:17:44: 10000000 pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 08:17:49: 12000000 INFO @ Sat, 17 Apr 2021 08:17:50: 11000000 INFO @ Sat, 17 Apr 2021 08:17:57: 12000000 INFO @ Sat, 17 Apr 2021 08:17:58: 13000000 INFO @ Sat, 17 Apr 2021 08:18:04: 13000000 INFO @ Sat, 17 Apr 2021 08:18:07: 14000000 INFO @ Sat, 17 Apr 2021 08:18:10: 14000000 INFO @ Sat, 17 Apr 2021 08:18:13: #1 tag size is determined as 51 bps INFO @ Sat, 17 Apr 2021 08:18:13: #1 tag size = 51 INFO @ Sat, 17 Apr 2021 08:18:13: #1 total tags in treatment: 6562181 INFO @ Sat, 17 Apr 2021 08:18:13: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 08:18:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 08:18:13: #1 tags after filtering in treatment: 3193603 INFO @ Sat, 17 Apr 2021 08:18:13: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 17 Apr 2021 08:18:13: #1 finished! INFO @ Sat, 17 Apr 2021 08:18:13: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 08:18:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 08:18:14: #2 number of paired peaks: 35 WARNING @ Sat, 17 Apr 2021 08:18:14: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 08:18:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 08:18:15: #1 tag size is determined as 51 bps INFO @ Sat, 17 Apr 2021 08:18:15: #1 tag size = 51 INFO @ Sat, 17 Apr 2021 08:18:15: #1 total tags in treatment: 6562181 INFO @ Sat, 17 Apr 2021 08:18:15: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 08:18:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 08:18:15: #1 tags after filtering in treatment: 3193603 INFO @ Sat, 17 Apr 2021 08:18:15: #1 Redundant rate of treatment: 0.51 INFO @ Sat, 17 Apr 2021 08:18:15: #1 finished! INFO @ Sat, 17 Apr 2021 08:18:15: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 08:18:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 08:18:15: #2 number of paired peaks: 35 WARNING @ Sat, 17 Apr 2021 08:18:15: Too few paired peaks (35) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 08:18:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797025/SRX2797025.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling