Job ID = 12531513 SRX = SRX2797023 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 11493984 spots for SRR5527837/SRR5527837.sra Written 11493984 spots for SRR5527837/SRR5527837.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Warning: skipping mate #1 of read 'SRR5527837.28080 28080 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.28080 28080 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.52478 52478 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.52478 52478 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.122011 122011 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.122011 122011 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.164162 164162 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.164162 164162 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.178767 178767 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.178767 178767 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.211999 211999 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.211999 211999 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.228540 228540 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.228540 228540 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.232065 232065 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.232065 232065 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.283804 283804 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.283804 283804 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.293802 293802 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.293802 293802 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.326533 326533 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.326533 326533 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.331569 331569 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.331569 331569 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.341864 341864 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.341864 341864 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.345088 345088 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.345088 345088 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.358217 358217 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.358217 358217 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.366574 366574 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.366574 366574 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.458111 458111 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.458111 458111 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.499795 499795 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.499795 499795 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.506554 506554 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.506554 506554 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.535846 535846 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.535846 535846 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.548375 548375 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.548375 548375 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.564537 564537 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.564537 564537 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.599400 599400 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.599400 599400 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.607313 607313 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.607313 607313 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.661540 661540 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.661540 661540 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.661818 661818 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.661818 661818 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.722961 722961 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.722961 722961 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.767376 767376 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.767376 767376 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.773723 773723 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.773723 773723 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.800120 800120 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.800120 800120 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.809900 809900 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.809900 809900 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.874078 874078 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.874078 874078 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.906928 906928 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.906928 906928 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1035155 1035155 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1035155 1035155 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1079328 1079328 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1079328 1079328 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1148860 1148860 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1148860 1148860 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.1223457 1223457 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.1223457 1223457 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.1229948 1229948 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.1229948 1229948 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1263848 1263848 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1263848 1263848 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.1293829 1293829 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.1293829 1293829 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1324007 1324007 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1324007 1324007 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.1431053 1431053 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.1431053 1431053 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1461616 1461616 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1461616 1461616 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.1492711 1492711 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.1492711 1492711 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1495173 1495173 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1495173 1495173 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.1509478 1509478 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.1509478 1509478 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1599225 1599225 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1599225 1599225 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1621763 1621763 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1621763 1621763 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1701123 1701123 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1701123 1701123 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1702066 1702066 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1702066 1702066 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.1710865 1710865 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.1710865 1710865 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.1793960 1793960 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.1793960 1793960 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.1881809 1881809 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.1881809 1881809 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1947681 1947681 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1947681 1947681 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1952658 1952658 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1952658 1952658 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.1954909 1954909 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.1954909 1954909 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.2021118 2021118 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.2021118 2021118 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.2073173 2073173 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.2073173 2073173 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.2080537 2080537 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.2080537 2080537 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.2087002 2087002 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.2087002 2087002 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.2129337 2129337 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.2129337 2129337 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.2178166 2178166 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.2178166 2178166 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.2194126 2194126 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.2194126 2194126 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.2201928 2201928 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.2201928 2201928 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.2213182 2213182 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.2213182 2213182 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.2252525 2252525 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.2252525 2252525 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.2324756 2324756 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.2324756 2324756 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.2480825 2480825 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.2480825 2480825 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.2638022 2638022 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.2638022 2638022 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.2856543 2856543 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.2856543 2856543 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.2954720 2954720 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.2954720 2954720 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3098256 3098256 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3098256 3098256 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3153072 3153072 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3153072 3153072 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3195060 3195060 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3195060 3195060 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3251837 3251837 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3251837 3251837 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3308487 3308487 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3308487 3308487 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3343774 3343774 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3343774 3343774 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3376113 3376113 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3376113 3376113 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.3471463 3471463 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.3471463 3471463 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.3525222 3525222 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.3525222 3525222 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.3583409 3583409 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.3583409 3583409 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3712918 3712918 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3712918 3712918 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3797178 3797178 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3797178 3797178 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3829281 3829281 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3829281 3829281 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3859270 3859270 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3859270 3859270 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3926769 3926769 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3926769 3926769 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.3933820 3933820 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.3933820 3933820 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4032805 4032805 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4032805 4032805 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4045160 4045160 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4045160 4045160 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4079834 4079834 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4079834 4079834 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4084013 4084013 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4084013 4084013 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4141604 4141604 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4141604 4141604 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4214909 4214909 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4214909 4214909 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4240091 4240091 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4240091 4240091 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4365475 4365475 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4365475 4365475 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4460757 4460757 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4460757 4460757 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4470813 4470813 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4470813 4470813 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4477252 4477252 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4477252 4477252 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4533025 4533025 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4533025 4533025 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4563836 4563836 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4563836 4563836 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4580921 4580921 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4580921 4580921 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4608196 4608196 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4608196 4608196 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4618131 4618131 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4618131 4618131 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4634839 4634839 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4634839 4634839 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4647541 4647541 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4647541 4647541 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4699292 4699292 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4699292 4699292 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4784788 4784788 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4784788 4784788 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4795087 4795087 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4795087 4795087 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.4804057 4804057 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.4804057 4804057 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.4948356 4948356 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.4948356 4948356 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5111432 5111432 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5111432 5111432 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5120269 5120269 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5120269 5120269 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5146778 5146778 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5146778 5146778 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.5200165 5200165 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.5200165 5200165 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5270156 5270156 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5270156 5270156 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.5330566 5330566 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.5330566 5330566 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5357668 5357668 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5357668 5357668 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.5405617 5405617 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.5405617 5405617 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.5426776 5426776 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.5426776 5426776 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5442159 5442159 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5442159 5442159 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5454433 5454433 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5454433 5454433 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5568797 5568797 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5568797 5568797 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5631526 5631526 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5631526 5631526 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.5633334 5633334 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.5633334 5633334 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.5696253 5696253 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.5696253 5696253 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5765132 5765132 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5765132 5765132 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5780517 5780517 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5780517 5780517 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.5832491 5832491 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.5832491 5832491 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.5867721 5867721 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.5867721 5867721 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.5934965 5934965 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.5934965 5934965 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.5970622 5970622 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.5970622 5970622 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6051257 6051257 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6051257 6051257 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6163798 6163798 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6163798 6163798 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6197916 6197916 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6197916 6197916 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.6282554 6282554 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.6282554 6282554 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.6332959 6332959 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.6332959 6332959 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6369570 6369570 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6369570 6369570 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6375029 6375029 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6375029 6375029 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.6436618 6436618 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.6436618 6436618 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.6575070 6575070 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.6575070 6575070 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6610797 6610797 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6610797 6610797 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6627606 6627606 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6627606 6627606 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.6687529 6687529 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.6687529 6687529 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6778991 6778991 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6778991 6778991 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.6808776 6808776 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.6808776 6808776 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.6813670 6813670 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.6813670 6813670 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6913562 6913562 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6913562 6913562 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.6932224 6932224 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.6932224 6932224 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.6984535 6984535 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.6984535 6984535 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7021526 7021526 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7021526 7021526 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.7052058 7052058 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.7052058 7052058 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7090960 7090960 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7090960 7090960 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.7127618 7127618 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.7127618 7127618 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7138287 7138287 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.7138287 7138287 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7138287 7138287 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.7138287 7138287 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.7169470 7169470 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.7169470 7169470 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7209766 7209766 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7209766 7209766 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7357251 7357251 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7357251 7357251 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7401464 7401464 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7401464 7401464 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7523963 7523963 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7523963 7523963 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7574237 7574237 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7574237 7574237 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7685259 7685259 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7685259 7685259 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.7698923 7698923 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.7698923 7698923 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7714673 7714673 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7714673 7714673 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7788594 7788594 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.7788594 7788594 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7788594 7788594 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.7788594 7788594 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.7830000 7830000 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.7830000 7830000 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7851655 7851655 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7851655 7851655 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7886766 7886766 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7886766 7886766 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.7967341 7967341 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.7967341 7967341 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.8038728 8038728 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.8038728 8038728 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.8057407 8057407 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.8057407 8057407 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.8114077 8114077 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.8114077 8114077 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.8263024 8263024 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.8263024 8263024 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.8275181 8275181 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.8275181 8275181 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.8303698 8303698 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.8303698 8303698 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.8412132 8412132 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.8412132 8412132 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.8425975 8425975 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.8425975 8425975 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.8505769 8505769 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.8505769 8505769 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.8729547 8729547 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.8729547 8729547 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.8849554 8849554 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.8849554 8849554 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.8880013 8880013 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.8880013 8880013 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.8898355 8898355 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.8898355 8898355 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.8932958 8932958 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.8932958 8932958 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.8964230 8964230 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.8964230 8964230 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9110614 9110614 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9110614 9110614 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9124381 9124381 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9124381 9124381 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.9132689 9132689 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.9132689 9132689 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9145804 9145804 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.9145804 9145804 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9145804 9145804 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.9145804 9145804 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9180146 9180146 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9180146 9180146 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.9207848 9207848 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.9207848 9207848 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.9213939 9213939 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.9213939 9213939 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.9373639 9373639 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.9373639 9373639 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9407680 9407680 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9407680 9407680 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9465780 9465780 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9465780 9465780 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.9571330 9571330 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.9571330 9571330 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9590785 9590785 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9590785 9590785 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9626837 9626837 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9626837 9626837 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.9631304 9631304 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.9631304 9631304 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9662105 9662105 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9662105 9662105 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9838972 9838972 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9838972 9838972 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9877491 9877491 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9877491 9877491 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9910968 9910968 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9910968 9910968 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.9957749 9957749 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.9957749 9957749 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.9991136 9991136 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.9991136 9991136 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.10210659 10210659 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.10210659 10210659 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.10280089 10280089 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.10280089 10280089 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.10450730 10450730 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.10450730 10450730 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.10537254 10537254 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.10537254 10537254 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.10571102 10571102 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.10571102 10571102 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.10660984 10660984 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.10660984 10660984 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.10674639 10674639 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.10674639 10674639 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.10685512 10685512 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.10685512 10685512 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.10812146 10812146 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.10812146 10812146 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.10832026 10832026 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.10832026 10832026 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.10855432 10855432 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.10855432 10855432 length=1' because it was < 2 characters long Warning: skipping mate #1 of read 'SRR5527837.11020535 11020535 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #1 of read 'SRR5527837.11020535 11020535 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.11166775 11166775 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.11166775 11166775 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.11210051 11210051 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.11210051 11210051 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.11377067 11377067 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.11377067 11377067 length=1' because it was < 2 characters long Warning: skipping mate #2 of read 'SRR5527837.11459080 11459080 length=1' because length (1) <= # seed mismatches (0) Warning: skipping mate #2 of read 'SRR5527837.11459080 11459080 length=1' because it was < 2 characters long Multiseed full-index search: 00:07:45 11493984 reads; of these: 11493984 (100.00%) were paired; of these: 1685328 (14.66%) aligned concordantly 0 times 5816609 (50.61%) aligned concordantly exactly 1 time 3992047 (34.73%) aligned concordantly >1 times ---- 1685328 pairs aligned concordantly 0 times; of these: 646536 (38.36%) aligned discordantly 1 time ---- 1038792 pairs aligned 0 times concordantly or discordantly; of these: 2077584 mates make up the pairs; of these: 1165857 (56.12%) aligned 0 times 116201 (5.59%) aligned exactly 1 time 795526 (38.29%) aligned >1 times 94.93% overall alignment rate Time searching: 00:07:45 Overall time: 00:07:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 3257730 / 10389421 = 0.3136 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 08:13:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 08:13:38: #1 read tag files... INFO @ Sat, 17 Apr 2021 08:13:38: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 08:13:46: 1000000 INFO @ Sat, 17 Apr 2021 08:13:55: 2000000 INFO @ Sat, 17 Apr 2021 08:14:03: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 08:14:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 08:14:07: #1 read tag files... INFO @ Sat, 17 Apr 2021 08:14:07: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 08:14:13: 4000000 INFO @ Sat, 17 Apr 2021 08:14:18: 1000000 INFO @ Sat, 17 Apr 2021 08:14:23: 5000000 INFO @ Sat, 17 Apr 2021 08:14:27: 2000000 INFO @ Sat, 17 Apr 2021 08:14:33: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.7.1/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 17 Apr 2021 08:14:37: 3000000 INFO @ Sat, 17 Apr 2021 08:14:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 17 Apr 2021 08:14:37: #1 read tag files... INFO @ Sat, 17 Apr 2021 08:14:37: #1 read treatment tags... INFO @ Sat, 17 Apr 2021 08:14:43: 7000000 INFO @ Sat, 17 Apr 2021 08:14:47: 4000000 INFO @ Sat, 17 Apr 2021 08:14:48: 1000000 INFO @ Sat, 17 Apr 2021 08:14:54: 8000000 INFO @ Sat, 17 Apr 2021 08:14:58: 5000000 INFO @ Sat, 17 Apr 2021 08:14:58: 2000000 INFO @ Sat, 17 Apr 2021 08:15:05: 9000000 INFO @ Sat, 17 Apr 2021 08:15:08: 3000000 INFO @ Sat, 17 Apr 2021 08:15:08: 6000000 INFO @ Sat, 17 Apr 2021 08:15:15: 10000000 INFO @ Sat, 17 Apr 2021 08:15:18: 4000000 INFO @ Sat, 17 Apr 2021 08:15:19: 7000000 INFO @ Sat, 17 Apr 2021 08:15:26: 11000000 INFO @ Sat, 17 Apr 2021 08:15:29: 5000000 INFO @ Sat, 17 Apr 2021 08:15:30: 8000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 17 Apr 2021 08:15:37: 12000000 INFO @ Sat, 17 Apr 2021 08:15:40: 6000000 INFO @ Sat, 17 Apr 2021 08:15:41: 9000000 INFO @ Sat, 17 Apr 2021 08:15:47: 13000000 BigWig に変換しました。 INFO @ Sat, 17 Apr 2021 08:15:51: 7000000 INFO @ Sat, 17 Apr 2021 08:15:52: 10000000 INFO @ Sat, 17 Apr 2021 08:15:57: 14000000 INFO @ Sat, 17 Apr 2021 08:16:02: 8000000 INFO @ Sat, 17 Apr 2021 08:16:03: 11000000 INFO @ Sat, 17 Apr 2021 08:16:08: 15000000 INFO @ Sat, 17 Apr 2021 08:16:11: #1 tag size is determined as 51 bps INFO @ Sat, 17 Apr 2021 08:16:11: #1 tag size = 51 INFO @ Sat, 17 Apr 2021 08:16:11: #1 total tags in treatment: 6595670 INFO @ Sat, 17 Apr 2021 08:16:11: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 08:16:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 08:16:11: #1 tags after filtering in treatment: 3302503 INFO @ Sat, 17 Apr 2021 08:16:11: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 17 Apr 2021 08:16:11: #1 finished! INFO @ Sat, 17 Apr 2021 08:16:11: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 08:16:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 08:16:11: #2 number of paired peaks: 29 WARNING @ Sat, 17 Apr 2021 08:16:11: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 08:16:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 08:16:13: 9000000 INFO @ Sat, 17 Apr 2021 08:16:14: 12000000 INFO @ Sat, 17 Apr 2021 08:16:23: 10000000 INFO @ Sat, 17 Apr 2021 08:16:24: 13000000 INFO @ Sat, 17 Apr 2021 08:16:32: 11000000 INFO @ Sat, 17 Apr 2021 08:16:33: 14000000 INFO @ Sat, 17 Apr 2021 08:16:42: 12000000 INFO @ Sat, 17 Apr 2021 08:16:43: 15000000 INFO @ Sat, 17 Apr 2021 08:16:46: #1 tag size is determined as 51 bps INFO @ Sat, 17 Apr 2021 08:16:46: #1 tag size = 51 INFO @ Sat, 17 Apr 2021 08:16:46: #1 total tags in treatment: 6595670 INFO @ Sat, 17 Apr 2021 08:16:46: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 08:16:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 08:16:46: #1 tags after filtering in treatment: 3302503 INFO @ Sat, 17 Apr 2021 08:16:46: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 17 Apr 2021 08:16:46: #1 finished! INFO @ Sat, 17 Apr 2021 08:16:46: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 08:16:46: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 08:16:46: #2 number of paired peaks: 29 WARNING @ Sat, 17 Apr 2021 08:16:46: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 08:16:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 17 Apr 2021 08:16:51: 13000000 INFO @ Sat, 17 Apr 2021 08:17:00: 14000000 INFO @ Sat, 17 Apr 2021 08:17:08: 15000000 INFO @ Sat, 17 Apr 2021 08:17:11: #1 tag size is determined as 51 bps INFO @ Sat, 17 Apr 2021 08:17:11: #1 tag size = 51 INFO @ Sat, 17 Apr 2021 08:17:11: #1 total tags in treatment: 6595670 INFO @ Sat, 17 Apr 2021 08:17:11: #1 user defined the maximum tags... INFO @ Sat, 17 Apr 2021 08:17:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 17 Apr 2021 08:17:11: #1 tags after filtering in treatment: 3302503 INFO @ Sat, 17 Apr 2021 08:17:11: #1 Redundant rate of treatment: 0.50 INFO @ Sat, 17 Apr 2021 08:17:11: #1 finished! INFO @ Sat, 17 Apr 2021 08:17:11: #2 Build Peak Model... INFO @ Sat, 17 Apr 2021 08:17:11: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 17 Apr 2021 08:17:11: #2 number of paired peaks: 29 WARNING @ Sat, 17 Apr 2021 08:17:11: Too few paired peaks (29) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 17 Apr 2021 08:17:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797023/SRX2797023.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling