Job ID = 7098188 SRX = SRX2797012 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 6087922 spots for SRR5527826/SRR5527826.sra Written 6087922 spots for SRR5527826/SRR5527826.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:39 6087922 reads; of these: 6087922 (100.00%) were unpaired; of these: 105698 (1.74%) aligned 0 times 3528000 (57.95%) aligned exactly 1 time 2454224 (40.31%) aligned >1 times 98.26% overall alignment rate Time searching: 00:00:39 Overall time: 00:00:39 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3303885 / 5982224 = 0.5523 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:54:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:54:09: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:54:09: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:54:15: 1000000 INFO @ Wed, 22 Jul 2020 11:54:21: 2000000 INFO @ Wed, 22 Jul 2020 11:54:25: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:54:25: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:54:25: #1 total tags in treatment: 2678339 INFO @ Wed, 22 Jul 2020 11:54:25: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:54:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:54:25: #1 tags after filtering in treatment: 2678339 INFO @ Wed, 22 Jul 2020 11:54:25: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:54:25: #1 finished! INFO @ Wed, 22 Jul 2020 11:54:25: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:54:25: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:54:25: #2 number of paired peaks: 30 WARNING @ Wed, 22 Jul 2020 11:54:25: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:54:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:54:38: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:54:38: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:54:38: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:54:44: 1000000 INFO @ Wed, 22 Jul 2020 11:54:50: 2000000 INFO @ Wed, 22 Jul 2020 11:54:54: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:54:54: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:54:54: #1 total tags in treatment: 2678339 INFO @ Wed, 22 Jul 2020 11:54:54: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:54:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:54:54: #1 tags after filtering in treatment: 2678339 INFO @ Wed, 22 Jul 2020 11:54:54: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:54:54: #1 finished! INFO @ Wed, 22 Jul 2020 11:54:54: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:54:54: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:54:54: #2 number of paired peaks: 30 WARNING @ Wed, 22 Jul 2020 11:54:54: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:54:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:55:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:55:08: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:55:08: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:55:14: 1000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 11:55:20: 2000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 11:55:24: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:55:24: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:55:24: #1 total tags in treatment: 2678339 INFO @ Wed, 22 Jul 2020 11:55:24: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:55:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:55:24: #1 tags after filtering in treatment: 2678339 INFO @ Wed, 22 Jul 2020 11:55:24: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:55:24: #1 finished! INFO @ Wed, 22 Jul 2020 11:55:24: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:55:24: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:55:24: #2 number of paired peaks: 30 WARNING @ Wed, 22 Jul 2020 11:55:24: Too few paired peaks (30) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:55:24: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797012/SRX2797012.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling