Job ID = 7098173 SRX = SRX2797011 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8120054 spots for SRR5527825/SRR5527825.sra Written 8120054 spots for SRR5527825/SRR5527825.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:11 8120054 reads; of these: 8120054 (100.00%) were unpaired; of these: 155439 (1.91%) aligned 0 times 6500049 (80.05%) aligned exactly 1 time 1464566 (18.04%) aligned >1 times 98.09% overall alignment rate Time searching: 00:01:11 Overall time: 00:01:11 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3374696 / 7964615 = 0.4237 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:54:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:54:30: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:54:30: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:54:36: 1000000 INFO @ Wed, 22 Jul 2020 11:54:42: 2000000 INFO @ Wed, 22 Jul 2020 11:54:47: 3000000 INFO @ Wed, 22 Jul 2020 11:54:53: 4000000 INFO @ Wed, 22 Jul 2020 11:54:56: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:54:56: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:54:56: #1 total tags in treatment: 4589919 INFO @ Wed, 22 Jul 2020 11:54:56: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:54:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:54:56: #1 tags after filtering in treatment: 4589919 INFO @ Wed, 22 Jul 2020 11:54:56: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:54:56: #1 finished! INFO @ Wed, 22 Jul 2020 11:54:56: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:54:56: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:54:56: #2 number of paired peaks: 27 WARNING @ Wed, 22 Jul 2020 11:54:56: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:54:56: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:55:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:55:01: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:55:01: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:55:07: 1000000 INFO @ Wed, 22 Jul 2020 11:55:12: 2000000 INFO @ Wed, 22 Jul 2020 11:55:18: 3000000 INFO @ Wed, 22 Jul 2020 11:55:23: 4000000 INFO @ Wed, 22 Jul 2020 11:55:26: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:55:26: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:55:26: #1 total tags in treatment: 4589919 INFO @ Wed, 22 Jul 2020 11:55:26: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:55:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:55:26: #1 tags after filtering in treatment: 4589919 INFO @ Wed, 22 Jul 2020 11:55:26: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:55:26: #1 finished! INFO @ Wed, 22 Jul 2020 11:55:26: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:55:26: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:55:27: #2 number of paired peaks: 27 WARNING @ Wed, 22 Jul 2020 11:55:27: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:55:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:55:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:55:30: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:55:30: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:55:36: 1000000 INFO @ Wed, 22 Jul 2020 11:55:42: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 11:55:48: 3000000 INFO @ Wed, 22 Jul 2020 11:55:54: 4000000 BigWig に変換しました。 INFO @ Wed, 22 Jul 2020 11:55:57: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:55:57: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:55:57: #1 total tags in treatment: 4589919 INFO @ Wed, 22 Jul 2020 11:55:57: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:55:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:55:57: #1 tags after filtering in treatment: 4589919 INFO @ Wed, 22 Jul 2020 11:55:57: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:55:57: #1 finished! INFO @ Wed, 22 Jul 2020 11:55:57: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:55:57: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:55:58: #2 number of paired peaks: 27 WARNING @ Wed, 22 Jul 2020 11:55:58: Too few paired peaks (27) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:55:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797011/SRX2797011.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling