Job ID = 7098038 SRX = SRX2797008 Genome = sacCer3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 8035081 spots for SRR5527822/SRR5527822.sra Written 8035081 spots for SRR5527822/SRR5527822.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:59 8035081 reads; of these: 8035081 (100.00%) were unpaired; of these: 191900 (2.39%) aligned 0 times 6268209 (78.01%) aligned exactly 1 time 1574972 (19.60%) aligned >1 times 97.61% overall alignment rate Time searching: 00:00:59 Overall time: 00:00:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 3574576 / 7843181 = 0.4558 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:52:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:52:23: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:52:23: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:52:28: 1000000 INFO @ Wed, 22 Jul 2020 11:52:33: 2000000 INFO @ Wed, 22 Jul 2020 11:52:38: 3000000 INFO @ Wed, 22 Jul 2020 11:52:44: 4000000 INFO @ Wed, 22 Jul 2020 11:52:45: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:52:45: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:52:45: #1 total tags in treatment: 4268605 INFO @ Wed, 22 Jul 2020 11:52:45: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:52:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:52:45: #1 tags after filtering in treatment: 4268605 INFO @ Wed, 22 Jul 2020 11:52:45: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:52:45: #1 finished! INFO @ Wed, 22 Jul 2020 11:52:45: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:52:45: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:52:45: #2 number of paired peaks: 33 WARNING @ Wed, 22 Jul 2020 11:52:45: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:52:45: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:52:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:52:53: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:52:53: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:52:58: 1000000 INFO @ Wed, 22 Jul 2020 11:53:03: 2000000 INFO @ Wed, 22 Jul 2020 11:53:08: 3000000 INFO @ Wed, 22 Jul 2020 11:53:14: 4000000 INFO @ Wed, 22 Jul 2020 11:53:15: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:53:15: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:53:15: #1 total tags in treatment: 4268605 INFO @ Wed, 22 Jul 2020 11:53:15: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:53:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:53:15: #1 tags after filtering in treatment: 4268605 INFO @ Wed, 22 Jul 2020 11:53:15: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:53:15: #1 finished! INFO @ Wed, 22 Jul 2020 11:53:15: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:53:15: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:53:15: #2 number of paired peaks: 33 WARNING @ Wed, 22 Jul 2020 11:53:15: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:53:15: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Wed, 22 Jul 2020 11:53:23: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Wed, 22 Jul 2020 11:53:23: #1 read tag files... INFO @ Wed, 22 Jul 2020 11:53:23: #1 read treatment tags... INFO @ Wed, 22 Jul 2020 11:53:28: 1000000 INFO @ Wed, 22 Jul 2020 11:53:33: 2000000 INFO @ Wed, 22 Jul 2020 11:53:38: 3000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Wed, 22 Jul 2020 11:53:43: 4000000 INFO @ Wed, 22 Jul 2020 11:53:44: #1 tag size is determined as 51 bps INFO @ Wed, 22 Jul 2020 11:53:44: #1 tag size = 51 INFO @ Wed, 22 Jul 2020 11:53:44: #1 total tags in treatment: 4268605 INFO @ Wed, 22 Jul 2020 11:53:44: #1 user defined the maximum tags... INFO @ Wed, 22 Jul 2020 11:53:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Wed, 22 Jul 2020 11:53:44: #1 tags after filtering in treatment: 4268605 INFO @ Wed, 22 Jul 2020 11:53:44: #1 Redundant rate of treatment: 0.00 INFO @ Wed, 22 Jul 2020 11:53:44: #1 finished! INFO @ Wed, 22 Jul 2020 11:53:44: #2 Build Peak Model... INFO @ Wed, 22 Jul 2020 11:53:44: #2 looking for paired plus/minus strand peaks... INFO @ Wed, 22 Jul 2020 11:53:44: #2 number of paired peaks: 33 WARNING @ Wed, 22 Jul 2020 11:53:44: Too few paired peaks (33) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Wed, 22 Jul 2020 11:53:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2797008/SRX2797008.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。