Job ID = 2010148 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 10,767,897 reads read : 10,767,897 reads written : 10,767,897 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:25 10767897 reads; of these: 10767897 (100.00%) were unpaired; of these: 6102283 (56.67%) aligned 0 times 3947070 (36.66%) aligned exactly 1 time 718544 (6.67%) aligned >1 times 43.33% overall alignment rate Time searching: 00:01:25 Overall time: 00:01:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 873501 / 4665614 = 0.1872 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:36:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:36:15: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:36:15: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:36:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:36:16: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:36:16: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:36:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:36:17: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:36:17: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:36:23: 1000000 INFO @ Fri, 05 Jul 2019 21:36:24: 1000000 INFO @ Fri, 05 Jul 2019 21:36:26: 1000000 INFO @ Fri, 05 Jul 2019 21:36:31: 2000000 INFO @ Fri, 05 Jul 2019 21:36:33: 2000000 INFO @ Fri, 05 Jul 2019 21:36:35: 2000000 INFO @ Fri, 05 Jul 2019 21:36:38: 3000000 INFO @ Fri, 05 Jul 2019 21:36:42: 3000000 INFO @ Fri, 05 Jul 2019 21:36:43: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:36:43: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:36:43: #1 total tags in treatment: 3792113 INFO @ Fri, 05 Jul 2019 21:36:43: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:36:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:36:44: #1 tags after filtering in treatment: 3792113 INFO @ Fri, 05 Jul 2019 21:36:44: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:36:44: #1 finished! INFO @ Fri, 05 Jul 2019 21:36:44: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:36:44: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:36:44: 3000000 INFO @ Fri, 05 Jul 2019 21:36:44: #2 number of paired peaks: 31 WARNING @ Fri, 05 Jul 2019 21:36:44: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:36:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:36:48: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:36:48: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:36:48: #1 total tags in treatment: 3792113 INFO @ Fri, 05 Jul 2019 21:36:48: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:36:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:36:48: #1 tags after filtering in treatment: 3792113 INFO @ Fri, 05 Jul 2019 21:36:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:36:48: #1 finished! INFO @ Fri, 05 Jul 2019 21:36:48: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:36:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:36:49: #2 number of paired peaks: 31 WARNING @ Fri, 05 Jul 2019 21:36:49: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:36:49: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:36:51: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:36:51: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:36:51: #1 total tags in treatment: 3792113 INFO @ Fri, 05 Jul 2019 21:36:51: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:36:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:36:51: #1 tags after filtering in treatment: 3792113 INFO @ Fri, 05 Jul 2019 21:36:51: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:36:51: #1 finished! INFO @ Fri, 05 Jul 2019 21:36:51: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:36:51: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:36:51: #2 number of paired peaks: 31 WARNING @ Fri, 05 Jul 2019 21:36:51: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:36:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772223/SRX2772223.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。