Job ID = 2010102 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,447,349 reads read : 36,894,698 reads written : 18,447,349 reads 0-length : 18,447,349 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:12 18447349 reads; of these: 18447349 (100.00%) were unpaired; of these: 9829282 (53.28%) aligned 0 times 7388562 (40.05%) aligned exactly 1 time 1229505 (6.66%) aligned >1 times 46.72% overall alignment rate Time searching: 00:02:12 Overall time: 00:02:12 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 2059418 / 8618067 = 0.2390 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:27:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:27:03: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:27:03: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:27:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:27:04: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:27:04: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:27:05: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:27:05: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:27:05: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:27:12: 1000000 INFO @ Fri, 05 Jul 2019 21:27:14: 1000000 INFO @ Fri, 05 Jul 2019 21:27:15: 1000000 INFO @ Fri, 05 Jul 2019 21:27:21: 2000000 INFO @ Fri, 05 Jul 2019 21:27:23: 2000000 INFO @ Fri, 05 Jul 2019 21:27:25: 2000000 INFO @ Fri, 05 Jul 2019 21:27:31: 3000000 INFO @ Fri, 05 Jul 2019 21:27:31: 3000000 INFO @ Fri, 05 Jul 2019 21:27:34: 3000000 INFO @ Fri, 05 Jul 2019 21:27:40: 4000000 INFO @ Fri, 05 Jul 2019 21:27:40: 4000000 INFO @ Fri, 05 Jul 2019 21:27:44: 4000000 INFO @ Fri, 05 Jul 2019 21:27:47: 5000000 INFO @ Fri, 05 Jul 2019 21:27:49: 5000000 INFO @ Fri, 05 Jul 2019 21:27:53: 5000000 INFO @ Fri, 05 Jul 2019 21:27:54: 6000000 INFO @ Fri, 05 Jul 2019 21:27:58: 6000000 INFO @ Fri, 05 Jul 2019 21:27:58: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:27:58: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:27:58: #1 total tags in treatment: 6558649 INFO @ Fri, 05 Jul 2019 21:27:58: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:27:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:27:58: #1 tags after filtering in treatment: 6558649 INFO @ Fri, 05 Jul 2019 21:27:58: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:27:58: #1 finished! INFO @ Fri, 05 Jul 2019 21:27:58: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:27:58: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:27:59: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:27:59: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:27:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:28:02: 6000000 INFO @ Fri, 05 Jul 2019 21:28:03: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:28:03: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:28:03: #1 total tags in treatment: 6558649 INFO @ Fri, 05 Jul 2019 21:28:03: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:28:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:28:03: #1 tags after filtering in treatment: 6558649 INFO @ Fri, 05 Jul 2019 21:28:03: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:28:03: #1 finished! INFO @ Fri, 05 Jul 2019 21:28:03: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:28:03: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:28:03: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:28:03: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:28:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:28:07: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:28:07: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:28:07: #1 total tags in treatment: 6558649 INFO @ Fri, 05 Jul 2019 21:28:07: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:28:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:28:07: #1 tags after filtering in treatment: 6558649 INFO @ Fri, 05 Jul 2019 21:28:07: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:28:07: #1 finished! INFO @ Fri, 05 Jul 2019 21:28:07: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:28:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:28:08: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:28:08: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:28:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772186/SRX2772186.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。