Job ID = 2010087 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 20,285,448 reads read : 40,570,896 reads written : 20,285,448 reads 0-length : 20,285,448 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:02:48 20285448 reads; of these: 20285448 (100.00%) were unpaired; of these: 7961085 (39.25%) aligned 0 times 10750980 (53.00%) aligned exactly 1 time 1573383 (7.76%) aligned >1 times 60.75% overall alignment rate Time searching: 00:02:48 Overall time: 00:02:48 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4873010 / 12324363 = 0.3954 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:25:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:25:47: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:25:47: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:25:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:25:48: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:25:48: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:25:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:25:49: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:25:49: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:25:55: 1000000 INFO @ Fri, 05 Jul 2019 21:25:57: 1000000 INFO @ Fri, 05 Jul 2019 21:25:57: 1000000 INFO @ Fri, 05 Jul 2019 21:26:03: 2000000 INFO @ Fri, 05 Jul 2019 21:26:05: 2000000 INFO @ Fri, 05 Jul 2019 21:26:05: 2000000 INFO @ Fri, 05 Jul 2019 21:26:11: 3000000 INFO @ Fri, 05 Jul 2019 21:26:15: 3000000 INFO @ Fri, 05 Jul 2019 21:26:15: 3000000 INFO @ Fri, 05 Jul 2019 21:26:19: 4000000 INFO @ Fri, 05 Jul 2019 21:26:22: 4000000 INFO @ Fri, 05 Jul 2019 21:26:23: 4000000 INFO @ Fri, 05 Jul 2019 21:26:26: 5000000 INFO @ Fri, 05 Jul 2019 21:26:29: 5000000 INFO @ Fri, 05 Jul 2019 21:26:31: 5000000 INFO @ Fri, 05 Jul 2019 21:26:34: 6000000 INFO @ Fri, 05 Jul 2019 21:26:37: 6000000 INFO @ Fri, 05 Jul 2019 21:26:39: 6000000 INFO @ Fri, 05 Jul 2019 21:26:41: 7000000 INFO @ Fri, 05 Jul 2019 21:26:44: 7000000 INFO @ Fri, 05 Jul 2019 21:26:45: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:26:45: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:26:45: #1 total tags in treatment: 7451353 INFO @ Fri, 05 Jul 2019 21:26:45: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:26:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:26:46: #1 tags after filtering in treatment: 7451353 INFO @ Fri, 05 Jul 2019 21:26:46: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:26:46: #1 finished! INFO @ Fri, 05 Jul 2019 21:26:46: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:26:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:26:46: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:26:46: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:26:46: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:26:47: 7000000 INFO @ Fri, 05 Jul 2019 21:26:47: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:26:47: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:26:47: #1 total tags in treatment: 7451353 INFO @ Fri, 05 Jul 2019 21:26:47: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:26:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:26:48: #1 tags after filtering in treatment: 7451353 INFO @ Fri, 05 Jul 2019 21:26:48: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:26:48: #1 finished! INFO @ Fri, 05 Jul 2019 21:26:48: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:26:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:26:48: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:26:48: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:26:48: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:26:50: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:26:50: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:26:50: #1 total tags in treatment: 7451353 INFO @ Fri, 05 Jul 2019 21:26:50: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:26:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:26:50: #1 tags after filtering in treatment: 7451353 INFO @ Fri, 05 Jul 2019 21:26:50: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:26:50: #1 finished! INFO @ Fri, 05 Jul 2019 21:26:50: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:26:50: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:26:51: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:26:51: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:26:51: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772175/SRX2772175.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。