Job ID = 2010079 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 28,209,228 reads read : 28,209,228 reads written : 28,209,228 rm: cannot remove ‘[DSE]RR*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:04 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:26 28209228 reads; of these: 28209228 (100.00%) were unpaired; of these: 11244966 (39.86%) aligned 0 times 14578509 (51.68%) aligned exactly 1 time 2385753 (8.46%) aligned >1 times 60.14% overall alignment rate Time searching: 00:10:30 Overall time: 00:10:32 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 8315095 / 16964262 = 0.4902 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 05 Jul 2019 21:42:07: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:42:07: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:42:07: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:42:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:42:08: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:42:08: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:42:09: # Command line: callpeak -t /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.bam -f BAM -g 12100000 -n /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 05 Jul 2019 21:42:09: #1 read tag files... INFO @ Fri, 05 Jul 2019 21:42:09: #1 read treatment tags... INFO @ Fri, 05 Jul 2019 21:42:17: 1000000 INFO @ Fri, 05 Jul 2019 21:42:18: 1000000 INFO @ Fri, 05 Jul 2019 21:42:19: 1000000 INFO @ Fri, 05 Jul 2019 21:42:27: 2000000 INFO @ Fri, 05 Jul 2019 21:42:27: 2000000 INFO @ Fri, 05 Jul 2019 21:42:28: 2000000 INFO @ Fri, 05 Jul 2019 21:42:35: 3000000 INFO @ Fri, 05 Jul 2019 21:42:36: 3000000 INFO @ Fri, 05 Jul 2019 21:42:38: 3000000 INFO @ Fri, 05 Jul 2019 21:42:43: 4000000 INFO @ Fri, 05 Jul 2019 21:42:46: 4000000 INFO @ Fri, 05 Jul 2019 21:42:47: 4000000 INFO @ Fri, 05 Jul 2019 21:42:51: 5000000 INFO @ Fri, 05 Jul 2019 21:42:55: 5000000 INFO @ Fri, 05 Jul 2019 21:42:57: 5000000 INFO @ Fri, 05 Jul 2019 21:42:59: 6000000 INFO @ Fri, 05 Jul 2019 21:43:05: 6000000 INFO @ Fri, 05 Jul 2019 21:43:06: 6000000 INFO @ Fri, 05 Jul 2019 21:43:07: 7000000 INFO @ Fri, 05 Jul 2019 21:43:14: 7000000 INFO @ Fri, 05 Jul 2019 21:43:15: 8000000 INFO @ Fri, 05 Jul 2019 21:43:16: 7000000 INFO @ Fri, 05 Jul 2019 21:43:20: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:43:20: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:43:20: #1 total tags in treatment: 8649167 INFO @ Fri, 05 Jul 2019 21:43:20: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:43:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:43:21: #1 tags after filtering in treatment: 8649167 INFO @ Fri, 05 Jul 2019 21:43:21: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:43:21: #1 finished! INFO @ Fri, 05 Jul 2019 21:43:21: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:43:21: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:43:21: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:43:21: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:43:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:43:24: 8000000 INFO @ Fri, 05 Jul 2019 21:43:25: 8000000 INFO @ Fri, 05 Jul 2019 21:43:30: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:43:30: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:43:30: #1 total tags in treatment: 8649167 INFO @ Fri, 05 Jul 2019 21:43:30: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:43:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:43:30: #1 tags after filtering in treatment: 8649167 INFO @ Fri, 05 Jul 2019 21:43:30: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:43:30: #1 finished! INFO @ Fri, 05 Jul 2019 21:43:30: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:43:30: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:43:30: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:43:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:43:30: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Fri, 05 Jul 2019 21:43:31: #1 tag size is determined as 50 bps INFO @ Fri, 05 Jul 2019 21:43:31: #1 tag size = 50 INFO @ Fri, 05 Jul 2019 21:43:31: #1 total tags in treatment: 8649167 INFO @ Fri, 05 Jul 2019 21:43:31: #1 user defined the maximum tags... INFO @ Fri, 05 Jul 2019 21:43:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 05 Jul 2019 21:43:31: #1 tags after filtering in treatment: 8649167 INFO @ Fri, 05 Jul 2019 21:43:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 05 Jul 2019 21:43:31: #1 finished! INFO @ Fri, 05 Jul 2019 21:43:31: #2 Build Peak Model... INFO @ Fri, 05 Jul 2019 21:43:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 05 Jul 2019 21:43:32: #2 number of paired peaks: 0 WARNING @ Fri, 05 Jul 2019 21:43:32: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 05 Jul 2019 21:43:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/sacCer3/SRX2772169/SRX2772169.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。