Job ID = 11192873 sra ファイルのダウンロード中... Completed: 804296K bytes transferred in 22 seconds (299483K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 6307927 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2767193/SRR5483849.sra Written 6307927 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2767193/SRR5483849.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:15 6307927 reads; of these: 6307927 (100.00%) were paired; of these: 381444 (6.05%) aligned concordantly 0 times 4892104 (77.55%) aligned concordantly exactly 1 time 1034379 (16.40%) aligned concordantly >1 times ---- 381444 pairs aligned concordantly 0 times; of these: 24549 (6.44%) aligned discordantly 1 time ---- 356895 pairs aligned 0 times concordantly or discordantly; of these: 713790 mates make up the pairs; of these: 509414 (71.37%) aligned 0 times 152255 (21.33%) aligned exactly 1 time 52121 (7.30%) aligned >1 times 95.96% overall alignment rate Time searching: 00:15:15 Overall time: 00:15:15 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 5621640 / 5684249 = 0.9890 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:33:04: # Command line: callpeak -t SRX2767193.bam -f BAM -g 12100000 -n SRX2767193.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2767193.10 # format = BAM # ChIP-seq file = ['SRX2767193.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:33:04: # Command line: callpeak -t SRX2767193.bam -f BAM -g 12100000 -n SRX2767193.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2767193.05 # format = BAM # ChIP-seq file = ['SRX2767193.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:33:04: # Command line: callpeak -t SRX2767193.bam -f BAM -g 12100000 -n SRX2767193.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2767193.20 # format = BAM # ChIP-seq file = ['SRX2767193.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:33:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:33:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:33:04: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:33:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:33:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:33:04: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:33:13: #1 tag size is determined as 151 bps INFO @ Sat, 15 Sep 2018 10:33:13: #1 tag size = 151 INFO @ Sat, 15 Sep 2018 10:33:13: #1 total tags in treatment: 320371 INFO @ Sat, 15 Sep 2018 10:33:13: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:33:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:33:13: #1 tags after filtering in treatment: 68205 INFO @ Sat, 15 Sep 2018 10:33:13: #1 Redundant rate of treatment: 0.79 INFO @ Sat, 15 Sep 2018 10:33:13: #1 finished! INFO @ Sat, 15 Sep 2018 10:33:13: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:33:13: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:33:13: #2 number of paired peaks: 250 WARNING @ Sat, 15 Sep 2018 10:33:13: Fewer paired peaks (250) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 250 pairs to build model! INFO @ Sat, 15 Sep 2018 10:33:13: start model_add_line... INFO @ Sat, 15 Sep 2018 10:33:13: start X-correlation... INFO @ Sat, 15 Sep 2018 10:33:13: end of X-cor INFO @ Sat, 15 Sep 2018 10:33:13: #2 finished! INFO @ Sat, 15 Sep 2018 10:33:13: #2 predicted fragment length is 179 bps INFO @ Sat, 15 Sep 2018 10:33:13: #2 alternative fragment length(s) may be 41,153,179,203,244,261,283,309,352,377,417,442,458,526,548 bps INFO @ Sat, 15 Sep 2018 10:33:13: #2.2 Generate R script for model : SRX2767193.10_model.r WARNING @ Sat, 15 Sep 2018 10:33:13: #2 Since the d (179) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 15 Sep 2018 10:33:13: #2 You may need to consider one of the other alternative d(s): 41,153,179,203,244,261,283,309,352,377,417,442,458,526,548 WARNING @ Sat, 15 Sep 2018 10:33:13: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 15 Sep 2018 10:33:13: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:33:13: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:33:13: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:33:14: #4 Write output xls file... SRX2767193.10_peaks.xls INFO @ Sat, 15 Sep 2018 10:33:14: #4 Write peak in narrowPeak format file... SRX2767193.10_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:33:14: #4 Write summits bed file... SRX2767193.10_summits.bed INFO @ Sat, 15 Sep 2018 10:33:14: Done! pass1 - making usageList (6 chroms): 1 millis pass2 - checking and writing primary data (8 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 15 Sep 2018 10:33:14: #1 tag size is determined as 151 bps INFO @ Sat, 15 Sep 2018 10:33:14: #1 tag size = 151 INFO @ Sat, 15 Sep 2018 10:33:14: #1 total tags in treatment: 320371 INFO @ Sat, 15 Sep 2018 10:33:14: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:33:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:33:14: #1 tags after filtering in treatment: 68205 INFO @ Sat, 15 Sep 2018 10:33:14: #1 Redundant rate of treatment: 0.79 INFO @ Sat, 15 Sep 2018 10:33:14: #1 finished! INFO @ Sat, 15 Sep 2018 10:33:14: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:33:14: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:33:14: #2 number of paired peaks: 250 WARNING @ Sat, 15 Sep 2018 10:33:14: Fewer paired peaks (250) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 250 pairs to build model! INFO @ Sat, 15 Sep 2018 10:33:14: start model_add_line... INFO @ Sat, 15 Sep 2018 10:33:14: start X-correlation... INFO @ Sat, 15 Sep 2018 10:33:14: end of X-cor INFO @ Sat, 15 Sep 2018 10:33:14: #2 finished! INFO @ Sat, 15 Sep 2018 10:33:14: #2 predicted fragment length is 179 bps INFO @ Sat, 15 Sep 2018 10:33:14: #2 alternative fragment length(s) may be 41,153,179,203,244,261,283,309,352,377,417,442,458,526,548 bps INFO @ Sat, 15 Sep 2018 10:33:14: #2.2 Generate R script for model : SRX2767193.20_model.r WARNING @ Sat, 15 Sep 2018 10:33:14: #2 Since the d (179) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 15 Sep 2018 10:33:14: #2 You may need to consider one of the other alternative d(s): 41,153,179,203,244,261,283,309,352,377,417,442,458,526,548 WARNING @ Sat, 15 Sep 2018 10:33:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 15 Sep 2018 10:33:14: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:33:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:33:15: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:33:15: #4 Write output xls file... SRX2767193.20_peaks.xls INFO @ Sat, 15 Sep 2018 10:33:15: #4 Write peak in narrowPeak format file... SRX2767193.20_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:33:15: #4 Write summits bed file... SRX2767193.20_summits.bed INFO @ Sat, 15 Sep 2018 10:33:15: Done! BedGraph に変換しました。 BigWig に変換中... pass1 - making usageList (2 chroms): 2 millis pass2 - checking and writing primary data (3 records, 4 fields): 2 millis CompletedMACS2peakCalling BigWig に変換しました。 INFO @ Sat, 15 Sep 2018 10:33:17: #1 tag size is determined as 151 bps INFO @ Sat, 15 Sep 2018 10:33:17: #1 tag size = 151 INFO @ Sat, 15 Sep 2018 10:33:17: #1 total tags in treatment: 320371 INFO @ Sat, 15 Sep 2018 10:33:17: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:33:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:33:17: #1 tags after filtering in treatment: 68205 INFO @ Sat, 15 Sep 2018 10:33:17: #1 Redundant rate of treatment: 0.79 INFO @ Sat, 15 Sep 2018 10:33:17: #1 finished! INFO @ Sat, 15 Sep 2018 10:33:17: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:33:17: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:33:17: #2 number of paired peaks: 250 WARNING @ Sat, 15 Sep 2018 10:33:17: Fewer paired peaks (250) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 250 pairs to build model! INFO @ Sat, 15 Sep 2018 10:33:17: start model_add_line... INFO @ Sat, 15 Sep 2018 10:33:17: start X-correlation... INFO @ Sat, 15 Sep 2018 10:33:17: end of X-cor INFO @ Sat, 15 Sep 2018 10:33:17: #2 finished! INFO @ Sat, 15 Sep 2018 10:33:17: #2 predicted fragment length is 179 bps INFO @ Sat, 15 Sep 2018 10:33:17: #2 alternative fragment length(s) may be 41,153,179,203,244,261,283,309,352,377,417,442,458,526,548 bps INFO @ Sat, 15 Sep 2018 10:33:17: #2.2 Generate R script for model : SRX2767193.05_model.r WARNING @ Sat, 15 Sep 2018 10:33:17: #2 Since the d (179) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 15 Sep 2018 10:33:17: #2 You may need to consider one of the other alternative d(s): 41,153,179,203,244,261,283,309,352,377,417,442,458,526,548 WARNING @ Sat, 15 Sep 2018 10:33:17: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 15 Sep 2018 10:33:17: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:33:17: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:33:18: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:33:18: #4 Write output xls file... SRX2767193.05_peaks.xls INFO @ Sat, 15 Sep 2018 10:33:18: #4 Write peak in narrowPeak format file... SRX2767193.05_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:33:18: #4 Write summits bed file... SRX2767193.05_summits.bed INFO @ Sat, 15 Sep 2018 10:33:18: Done! pass1 - making usageList (11 chroms): 2 millis pass2 - checking and writing primary data (26 records, 4 fields): 1 millis CompletedMACS2peakCalling