Job ID = 11192872 sra ファイルのダウンロード中... Completed: 1055512K bytes transferred in 16 seconds (516324K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: PAIRED fastq に変換中... Read 8549210 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2767192/SRR5483848.sra Written 8549210 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2767192/SRR5483848.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:14:30 8549210 reads; of these: 8549210 (100.00%) were paired; of these: 744535 (8.71%) aligned concordantly 0 times 6656442 (77.86%) aligned concordantly exactly 1 time 1148233 (13.43%) aligned concordantly >1 times ---- 744535 pairs aligned concordantly 0 times; of these: 61294 (8.23%) aligned discordantly 1 time ---- 683241 pairs aligned 0 times concordantly or discordantly; of these: 1366482 mates make up the pairs; of these: 1137538 (83.25%) aligned 0 times 165009 (12.08%) aligned exactly 1 time 63935 (4.68%) aligned >1 times 93.35% overall alignment rate Time searching: 00:14:30 Overall time: 00:14:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chrI... [bam_rmdup_core] processing reference chrII... [bam_rmdup_core] processing reference chrIII... [bam_rmdup_core] processing reference chrIV... [bam_rmdup_core] processing reference chrIX... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrV... [bam_rmdup_core] processing reference chrVI... [bam_rmdup_core] processing reference chrVII... [bam_rmdup_core] processing reference chrVIII... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXI... [bam_rmdup_core] processing reference chrXII... [bam_rmdup_core] processing reference chrXIII... [bam_rmdup_core] processing reference chrXIV... [bam_rmdup_core] processing reference chrXV... [bam_rmdup_core] processing reference chrXVI... [bam_rmdup_core] 7548720 / 7618173 = 0.9909 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sat, 15 Sep 2018 10:29:21: # Command line: callpeak -t SRX2767192.bam -f BAM -g 12100000 -n SRX2767192.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2767192.05 # format = BAM # ChIP-seq file = ['SRX2767192.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:29:21: # Command line: callpeak -t SRX2767192.bam -f BAM -g 12100000 -n SRX2767192.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2767192.20 # format = BAM # ChIP-seq file = ['SRX2767192.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:29:21: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:29:21: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:29:21: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:29:21: #1 read treatment tags... INFO @ Sat, 15 Sep 2018 10:29:21: # Command line: callpeak -t SRX2767192.bam -f BAM -g 12100000 -n SRX2767192.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2767192.10 # format = BAM # ChIP-seq file = ['SRX2767192.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 15 Sep 2018 10:29:21: #1 read tag files... INFO @ Sat, 15 Sep 2018 10:29:21: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Sat, 15 Sep 2018 10:29:29: #1 tag size is determined as 151 bps INFO @ Sat, 15 Sep 2018 10:29:29: #1 tag size = 151 INFO @ Sat, 15 Sep 2018 10:29:29: #1 total tags in treatment: 308020 INFO @ Sat, 15 Sep 2018 10:29:29: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:29:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:29:29: #1 tag size is determined as 151 bps INFO @ Sat, 15 Sep 2018 10:29:29: #1 tag size = 151 INFO @ Sat, 15 Sep 2018 10:29:29: #1 total tags in treatment: 308020 INFO @ Sat, 15 Sep 2018 10:29:29: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:29:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:29:29: #1 tag size is determined as 151 bps INFO @ Sat, 15 Sep 2018 10:29:29: #1 tag size = 151 INFO @ Sat, 15 Sep 2018 10:29:29: #1 total tags in treatment: 308020 INFO @ Sat, 15 Sep 2018 10:29:29: #1 user defined the maximum tags... INFO @ Sat, 15 Sep 2018 10:29:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 15 Sep 2018 10:29:29: #1 tags after filtering in treatment: 72520 INFO @ Sat, 15 Sep 2018 10:29:29: #1 Redundant rate of treatment: 0.76 INFO @ Sat, 15 Sep 2018 10:29:29: #1 finished! INFO @ Sat, 15 Sep 2018 10:29:29: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:29:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:29:29: #1 tags after filtering in treatment: 72520 INFO @ Sat, 15 Sep 2018 10:29:29: #1 Redundant rate of treatment: 0.76 INFO @ Sat, 15 Sep 2018 10:29:29: #1 finished! INFO @ Sat, 15 Sep 2018 10:29:29: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:29:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:29:29: #1 tags after filtering in treatment: 72520 INFO @ Sat, 15 Sep 2018 10:29:29: #1 Redundant rate of treatment: 0.76 INFO @ Sat, 15 Sep 2018 10:29:29: #1 finished! INFO @ Sat, 15 Sep 2018 10:29:29: #2 Build Peak Model... INFO @ Sat, 15 Sep 2018 10:29:29: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 15 Sep 2018 10:29:29: #2 number of paired peaks: 234 WARNING @ Sat, 15 Sep 2018 10:29:29: Fewer paired peaks (234) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 234 pairs to build model! INFO @ Sat, 15 Sep 2018 10:29:29: start model_add_line... INFO @ Sat, 15 Sep 2018 10:29:29: #2 number of paired peaks: 234 WARNING @ Sat, 15 Sep 2018 10:29:29: Fewer paired peaks (234) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 234 pairs to build model! INFO @ Sat, 15 Sep 2018 10:29:29: start model_add_line... INFO @ Sat, 15 Sep 2018 10:29:29: start X-correlation... INFO @ Sat, 15 Sep 2018 10:29:29: #2 number of paired peaks: 234 WARNING @ Sat, 15 Sep 2018 10:29:29: Fewer paired peaks (234) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 234 pairs to build model! INFO @ Sat, 15 Sep 2018 10:29:29: start model_add_line... INFO @ Sat, 15 Sep 2018 10:29:29: start X-correlation... INFO @ Sat, 15 Sep 2018 10:29:29: start X-correlation... INFO @ Sat, 15 Sep 2018 10:29:29: end of X-cor INFO @ Sat, 15 Sep 2018 10:29:29: end of X-cor INFO @ Sat, 15 Sep 2018 10:29:29: end of X-cor INFO @ Sat, 15 Sep 2018 10:29:29: #2 finished! INFO @ Sat, 15 Sep 2018 10:29:29: #2 finished! INFO @ Sat, 15 Sep 2018 10:29:29: #2 finished! INFO @ Sat, 15 Sep 2018 10:29:29: #2 predicted fragment length is 175 bps INFO @ Sat, 15 Sep 2018 10:29:29: #2 predicted fragment length is 175 bps INFO @ Sat, 15 Sep 2018 10:29:29: #2 predicted fragment length is 175 bps INFO @ Sat, 15 Sep 2018 10:29:29: #2 alternative fragment length(s) may be 12,47,84,113,133,175,206,236,251,266,294,314,340,368,398,438,460,505,539,561,588 bps INFO @ Sat, 15 Sep 2018 10:29:29: #2 alternative fragment length(s) may be 12,47,84,113,133,175,206,236,251,266,294,314,340,368,398,438,460,505,539,561,588 bps INFO @ Sat, 15 Sep 2018 10:29:29: #2 alternative fragment length(s) may be 12,47,84,113,133,175,206,236,251,266,294,314,340,368,398,438,460,505,539,561,588 bps INFO @ Sat, 15 Sep 2018 10:29:29: #2.2 Generate R script for model : SRX2767192.05_model.r INFO @ Sat, 15 Sep 2018 10:29:29: #2.2 Generate R script for model : SRX2767192.20_model.r INFO @ Sat, 15 Sep 2018 10:29:29: #2.2 Generate R script for model : SRX2767192.10_model.r WARNING @ Sat, 15 Sep 2018 10:29:29: #2 Since the d (175) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 15 Sep 2018 10:29:29: #2 You may need to consider one of the other alternative d(s): 12,47,84,113,133,175,206,236,251,266,294,314,340,368,398,438,460,505,539,561,588 WARNING @ Sat, 15 Sep 2018 10:29:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 15 Sep 2018 10:29:29: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:29:29: #3 Pre-compute pvalue-qvalue table... WARNING @ Sat, 15 Sep 2018 10:29:29: #2 Since the d (175) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 15 Sep 2018 10:29:29: #2 Since the d (175) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 15 Sep 2018 10:29:29: #2 You may need to consider one of the other alternative d(s): 12,47,84,113,133,175,206,236,251,266,294,314,340,368,398,438,460,505,539,561,588 WARNING @ Sat, 15 Sep 2018 10:29:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. WARNING @ Sat, 15 Sep 2018 10:29:29: #2 You may need to consider one of the other alternative d(s): 12,47,84,113,133,175,206,236,251,266,294,314,340,368,398,438,460,505,539,561,588 INFO @ Sat, 15 Sep 2018 10:29:29: #3 Call peaks... WARNING @ Sat, 15 Sep 2018 10:29:29: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 15 Sep 2018 10:29:29: #3 Call peaks... INFO @ Sat, 15 Sep 2018 10:29:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:29:29: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 15 Sep 2018 10:29:29: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:29:29: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:29:29: #3 Call peaks for each chromosome... INFO @ Sat, 15 Sep 2018 10:29:29: #4 Write output xls file... SRX2767192.10_peaks.xls INFO @ Sat, 15 Sep 2018 10:29:29: #4 Write peak in narrowPeak format file... SRX2767192.10_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:29:29: #4 Write summits bed file... SRX2767192.10_summits.bed INFO @ Sat, 15 Sep 2018 10:29:29: Done! INFO @ Sat, 15 Sep 2018 10:29:29: #4 Write output xls file... SRX2767192.05_peaks.xls INFO @ Sat, 15 Sep 2018 10:29:29: #4 Write peak in narrowPeak format file... SRX2767192.05_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:29:29: #4 Write summits bed file... SRX2767192.05_summits.bed INFO @ Sat, 15 Sep 2018 10:29:29: Done! INFO @ Sat, 15 Sep 2018 10:29:29: #4 Write output xls file... SRX2767192.20_peaks.xls INFO @ Sat, 15 Sep 2018 10:29:29: #4 Write peak in narrowPeak format file... SRX2767192.20_peaks.narrowPeak INFO @ Sat, 15 Sep 2018 10:29:29: #4 Write summits bed file... SRX2767192.20_summits.bed INFO @ Sat, 15 Sep 2018 10:29:29: Done! pass1 - making usageList (3 chroms)pass1 - making usageList (2 chroms)pass1 - making usageList (1 chroms): 2 millis : 2 millis : 2 millis pass2 - checking and writing primary data (1 records, 4 fields): 4 millis pass2 - checking and writing primary data (3 records, 4 fields): 4 millis pass2 - checking and writing primary data (6 records, 4 fields): 4 millis CompletedMACS2peakCalling CompletedMACS2peakCalling CompletedMACS2peakCalling