Job ID = 10045051 sra ファイルのダウンロード中... Completed: 99338K bytes transferred in 5 seconds (150671K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 5757182 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2766937/SRR5483584.sra Written 5757182 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:58 5757182 reads; of these: 5757182 (100.00%) were unpaired; of these: 990255 (17.20%) aligned 0 times 4323245 (75.09%) aligned exactly 1 time 443682 (7.71%) aligned >1 times 82.80% overall alignment rate Time searching: 00:00:58 Overall time: 00:00:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 1332870 / 4766927 = 0.2796 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 02 Oct 2017 14:39:36: # Command line: callpeak -t SRX2766937.bam -f BAM -g 12100000 -n SRX2766937.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2766937.05 # format = BAM # ChIP-seq file = ['SRX2766937.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 02 Oct 2017 14:39:36: #1 read tag files... INFO @ Mon, 02 Oct 2017 14:39:36: #1 read treatment tags... INFO @ Mon, 02 Oct 2017 14:39:36: # Command line: callpeak -t SRX2766937.bam -f BAM -g 12100000 -n SRX2766937.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2766937.20 # format = BAM # ChIP-seq file = ['SRX2766937.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 02 Oct 2017 14:39:36: #1 read tag files... INFO @ Mon, 02 Oct 2017 14:39:36: #1 read treatment tags... INFO @ Mon, 02 Oct 2017 14:39:36: # Command line: callpeak -t SRX2766937.bam -f BAM -g 12100000 -n SRX2766937.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2766937.10 # format = BAM # ChIP-seq file = ['SRX2766937.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 02 Oct 2017 14:39:36: #1 read tag files... INFO @ Mon, 02 Oct 2017 14:39:36: #1 read treatment tags... INFO @ Mon, 02 Oct 2017 14:39:42: 1000000 INFO @ Mon, 02 Oct 2017 14:39:42: 1000000 INFO @ Mon, 02 Oct 2017 14:39:44: 1000000 INFO @ Mon, 02 Oct 2017 14:39:48: 2000000 INFO @ Mon, 02 Oct 2017 14:39:48: 2000000 INFO @ Mon, 02 Oct 2017 14:39:51: 2000000 INFO @ Mon, 02 Oct 2017 14:39:54: 3000000 INFO @ Mon, 02 Oct 2017 14:39:54: 3000000 INFO @ Mon, 02 Oct 2017 14:39:56: #1 tag size is determined as 51 bps INFO @ Mon, 02 Oct 2017 14:39:56: #1 tag size = 51 INFO @ Mon, 02 Oct 2017 14:39:56: #1 total tags in treatment: 3434057 INFO @ Mon, 02 Oct 2017 14:39:56: #1 user defined the maximum tags... INFO @ Mon, 02 Oct 2017 14:39:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 02 Oct 2017 14:39:56: #1 tags after filtering in treatment: 3434057 INFO @ Mon, 02 Oct 2017 14:39:56: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 02 Oct 2017 14:39:56: #1 finished! INFO @ Mon, 02 Oct 2017 14:39:56: #2 Build Peak Model... INFO @ Mon, 02 Oct 2017 14:39:56: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 02 Oct 2017 14:39:56: #1 tag size is determined as 51 bps INFO @ Mon, 02 Oct 2017 14:39:56: #1 tag size = 51 INFO @ Mon, 02 Oct 2017 14:39:56: #1 total tags in treatment: 3434057 INFO @ Mon, 02 Oct 2017 14:39:56: #1 user defined the maximum tags... INFO @ Mon, 02 Oct 2017 14:39:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 02 Oct 2017 14:39:57: #2 number of paired peaks: 31 WARNING @ Mon, 02 Oct 2017 14:39:57: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 02 Oct 2017 14:39:57: Process for pairing-model is terminated! cat: SRX2766937.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766937.05_model.r': そのようなファイルやディレクトリはありません INFO @ Mon, 02 Oct 2017 14:39:57: #1 tags after filtering in treatment: 3434057 INFO @ Mon, 02 Oct 2017 14:39:57: #1 Redundant rate of treatment: 0.00 rm: INFO @ Mon, 02 Oct 2017 14:39:57: #1 finished! cannot remove `SRX2766937.05_*.xls': そのようなファイルやディレクトリはありません INFO @ Mon, 02 Oct 2017 14:39:57: #2 Build Peak Model... INFO @ Mon, 02 Oct 2017 14:39:57: #2 looking for paired plus/minus strand peaks... rm: cannot remove `SRX2766937.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 02 Oct 2017 14:39:57: #2 number of paired peaks: 31 WARNING @ Mon, 02 Oct 2017 14:39:57: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 02 Oct 2017 14:39:57: Process for pairing-model is terminated! cat: SRX2766937.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766937.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766937.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766937.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 02 Oct 2017 14:39:59: 3000000 INFO @ Mon, 02 Oct 2017 14:40:02: #1 tag size is determined as 51 bps INFO @ Mon, 02 Oct 2017 14:40:02: #1 tag size = 51 INFO @ Mon, 02 Oct 2017 14:40:02: #1 total tags in treatment: 3434057 INFO @ Mon, 02 Oct 2017 14:40:02: #1 user defined the maximum tags... INFO @ Mon, 02 Oct 2017 14:40:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 02 Oct 2017 14:40:02: #1 tags after filtering in treatment: 3434057 INFO @ Mon, 02 Oct 2017 14:40:02: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 02 Oct 2017 14:40:02: #1 finished! INFO @ Mon, 02 Oct 2017 14:40:02: #2 Build Peak Model... INFO @ Mon, 02 Oct 2017 14:40:02: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 02 Oct 2017 14:40:02: #2 number of paired peaks: 31 WARNING @ Mon, 02 Oct 2017 14:40:02: Too few paired peaks (31) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 02 Oct 2017 14:40:02: Process for pairing-model is terminated! cat: SRX2766937.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 5 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766937.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766937.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766937.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。