Job ID = 10045050 sra ファイルのダウンロード中... Completed: 94165K bytes transferred in 5 seconds (135836K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 5474212 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2766936/SRR5483583.sra Written 5474212 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:00:58 5474212 reads; of these: 5474212 (100.00%) were unpaired; of these: 170164 (3.11%) aligned 0 times 5010586 (91.53%) aligned exactly 1 time 293462 (5.36%) aligned >1 times 96.89% overall alignment rate Time searching: 00:00:58 Overall time: 00:00:58 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_rmdupse_core] 937561 / 5304048 = 0.1768 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 02 Oct 2017 14:39:44: # Command line: callpeak -t SRX2766936.bam -f BAM -g 12100000 -n SRX2766936.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2766936.20 # format = BAM # ChIP-seq file = ['SRX2766936.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 02 Oct 2017 14:39:44: #1 read tag files... INFO @ Mon, 02 Oct 2017 14:39:44: #1 read treatment tags... INFO @ Mon, 02 Oct 2017 14:39:44: # Command line: callpeak -t SRX2766936.bam -f BAM -g 12100000 -n SRX2766936.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2766936.10 # format = BAM # ChIP-seq file = ['SRX2766936.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 02 Oct 2017 14:39:44: #1 read tag files... INFO @ Mon, 02 Oct 2017 14:39:44: #1 read treatment tags... INFO @ Mon, 02 Oct 2017 14:39:44: # Command line: callpeak -t SRX2766936.bam -f BAM -g 12100000 -n SRX2766936.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2766936.05 # format = BAM # ChIP-seq file = ['SRX2766936.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 02 Oct 2017 14:39:44: #1 read tag files... INFO @ Mon, 02 Oct 2017 14:39:44: #1 read treatment tags... INFO @ Mon, 02 Oct 2017 14:39:51: 1000000 INFO @ Mon, 02 Oct 2017 14:39:51: 1000000 INFO @ Mon, 02 Oct 2017 14:39:51: 1000000 INFO @ Mon, 02 Oct 2017 14:39:57: 2000000 INFO @ Mon, 02 Oct 2017 14:39:58: 2000000 INFO @ Mon, 02 Oct 2017 14:39:58: 2000000 INFO @ Mon, 02 Oct 2017 14:40:04: 3000000 INFO @ Mon, 02 Oct 2017 14:40:05: 3000000 INFO @ Mon, 02 Oct 2017 14:40:05: 3000000 INFO @ Mon, 02 Oct 2017 14:40:10: 4000000 INFO @ Mon, 02 Oct 2017 14:40:12: 4000000 INFO @ Mon, 02 Oct 2017 14:40:12: 4000000 INFO @ Mon, 02 Oct 2017 14:40:13: #1 tag size is determined as 51 bps INFO @ Mon, 02 Oct 2017 14:40:13: #1 tag size = 51 INFO @ Mon, 02 Oct 2017 14:40:13: #1 total tags in treatment: 4366487 INFO @ Mon, 02 Oct 2017 14:40:13: #1 user defined the maximum tags... INFO @ Mon, 02 Oct 2017 14:40:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 02 Oct 2017 14:40:13: #1 tags after filtering in treatment: 4366487 INFO @ Mon, 02 Oct 2017 14:40:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 02 Oct 2017 14:40:13: #1 finished! INFO @ Mon, 02 Oct 2017 14:40:13: #2 Build Peak Model... INFO @ Mon, 02 Oct 2017 14:40:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 02 Oct 2017 14:40:13: #2 number of paired peaks: 0 WARNING @ Mon, 02 Oct 2017 14:40:13: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 02 Oct 2017 14:40:13: Process for pairing-model is terminated! cat: SRX2766936.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 12 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766936.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766936.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766936.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 02 Oct 2017 14:40:14: #1 tag size is determined as 51 bps INFO @ Mon, 02 Oct 2017 14:40:14: #1 tag size = 51 INFO @ Mon, 02 Oct 2017 14:40:14: #1 total tags in treatment: 4366487 INFO @ Mon, 02 Oct 2017 14:40:14: #1 user defined the maximum tags... INFO @ Mon, 02 Oct 2017 14:40:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 02 Oct 2017 14:40:14: #1 tags after filtering in treatment: 4366487 INFO @ Mon, 02 Oct 2017 14:40:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 02 Oct 2017 14:40:14: #1 finished! INFO @ Mon, 02 Oct 2017 14:40:14: #2 Build Peak Model... INFO @ Mon, 02 Oct 2017 14:40:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 02 Oct 2017 14:40:14: #2 number of paired peaks: 0 WARNING @ Mon, 02 Oct 2017 14:40:14: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 02 Oct 2017 14:40:14: Process for pairing-model is terminated! cat: SRX2766936.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766936.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766936.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766936.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 02 Oct 2017 14:40:15: #1 tag size is determined as 51 bps INFO @ Mon, 02 Oct 2017 14:40:15: #1 tag size = 51 INFO @ Mon, 02 Oct 2017 14:40:15: #1 total tags in treatment: 4366487 INFO @ Mon, 02 Oct 2017 14:40:15: #1 user defined the maximum tags... INFO @ Mon, 02 Oct 2017 14:40:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 02 Oct 2017 14:40:15: #1 tags after filtering in treatment: 4366487 INFO @ Mon, 02 Oct 2017 14:40:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 02 Oct 2017 14:40:15: #1 finished! INFO @ Mon, 02 Oct 2017 14:40:15: #2 Build Peak Model... INFO @ Mon, 02 Oct 2017 14:40:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 02 Oct 2017 14:40:15: #2 number of paired peaks: 0 WARNING @ Mon, 02 Oct 2017 14:40:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 02 Oct 2017 14:40:15: Process for pairing-model is terminated! cat: SRX2766936.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 4 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766936.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766936.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766936.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。