Job ID = 10721717 sra ファイルのダウンロード中... Completed: 665310K bytes transferred in 50 seconds (107233K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Read 21189856 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2766304/SRR5482932.sra Written 21189856 spots for /home/okishinya/chipatlas/results/sacCer3/SRX2766304/SRR5482932.sra rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:04:29 21189856 reads; of these: 21189856 (100.00%) were unpaired; of these: 831120 (3.92%) aligned 0 times 15902262 (75.05%) aligned exactly 1 time 4456474 (21.03%) aligned >1 times 96.08% overall alignment rate Time searching: 00:04:29 Overall time: 00:04:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 17 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13171316 / 20358736 = 0.6470 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 04 Jun 2018 03:48:33: # Command line: callpeak -t SRX2766304.bam -f BAM -g 12100000 -n SRX2766304.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX2766304.20 # format = BAM # ChIP-seq file = ['SRX2766304.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 04 Jun 2018 03:48:33: #1 read tag files... INFO @ Mon, 04 Jun 2018 03:48:33: #1 read treatment tags... INFO @ Mon, 04 Jun 2018 03:48:33: # Command line: callpeak -t SRX2766304.bam -f BAM -g 12100000 -n SRX2766304.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX2766304.05 # format = BAM # ChIP-seq file = ['SRX2766304.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 04 Jun 2018 03:48:33: #1 read tag files... INFO @ Mon, 04 Jun 2018 03:48:33: #1 read treatment tags... INFO @ Mon, 04 Jun 2018 03:48:33: # Command line: callpeak -t SRX2766304.bam -f BAM -g 12100000 -n SRX2766304.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX2766304.10 # format = BAM # ChIP-seq file = ['SRX2766304.bam'] # control file = None # effective genome size = 1.21e+07 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 04 Jun 2018 03:48:33: #1 read tag files... INFO @ Mon, 04 Jun 2018 03:48:33: #1 read treatment tags... INFO @ Mon, 04 Jun 2018 03:48:41: 1000000 INFO @ Mon, 04 Jun 2018 03:48:42: 1000000 INFO @ Mon, 04 Jun 2018 03:48:42: 1000000 INFO @ Mon, 04 Jun 2018 03:48:48: 2000000 INFO @ Mon, 04 Jun 2018 03:48:51: 2000000 INFO @ Mon, 04 Jun 2018 03:48:51: 2000000 INFO @ Mon, 04 Jun 2018 03:48:56: 3000000 INFO @ Mon, 04 Jun 2018 03:49:01: 3000000 INFO @ Mon, 04 Jun 2018 03:49:01: 3000000 INFO @ Mon, 04 Jun 2018 03:49:04: 4000000 INFO @ Mon, 04 Jun 2018 03:49:10: 4000000 INFO @ Mon, 04 Jun 2018 03:49:10: 4000000 INFO @ Mon, 04 Jun 2018 03:49:12: 5000000 INFO @ Mon, 04 Jun 2018 03:49:20: 5000000 INFO @ Mon, 04 Jun 2018 03:49:20: 5000000 INFO @ Mon, 04 Jun 2018 03:49:20: 6000000 INFO @ Mon, 04 Jun 2018 03:49:28: 7000000 INFO @ Mon, 04 Jun 2018 03:49:29: #1 tag size is determined as 50 bps INFO @ Mon, 04 Jun 2018 03:49:29: #1 tag size = 50 INFO @ Mon, 04 Jun 2018 03:49:29: #1 total tags in treatment: 7187420 INFO @ Mon, 04 Jun 2018 03:49:29: #1 user defined the maximum tags... INFO @ Mon, 04 Jun 2018 03:49:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 04 Jun 2018 03:49:29: 6000000 INFO @ Mon, 04 Jun 2018 03:49:29: #1 tags after filtering in treatment: 7187420 INFO @ Mon, 04 Jun 2018 03:49:29: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 04 Jun 2018 03:49:29: #1 finished! INFO @ Mon, 04 Jun 2018 03:49:29: #2 Build Peak Model... INFO @ Mon, 04 Jun 2018 03:49:29: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 04 Jun 2018 03:49:29: 6000000 INFO @ Mon, 04 Jun 2018 03:49:30: #2 number of paired peaks: 0 WARNING @ Mon, 04 Jun 2018 03:49:30: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 04 Jun 2018 03:49:30: Process for pairing-model is terminated! cat: SRX2766304.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766304.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766304.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766304.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 04 Jun 2018 03:49:39: 7000000 INFO @ Mon, 04 Jun 2018 03:49:39: 7000000 INFO @ Mon, 04 Jun 2018 03:49:40: #1 tag size is determined as 50 bps INFO @ Mon, 04 Jun 2018 03:49:40: #1 tag size = 50 INFO @ Mon, 04 Jun 2018 03:49:40: #1 total tags in treatment: 7187420 INFO @ Mon, 04 Jun 2018 03:49:40: #1 user defined the maximum tags... INFO @ Mon, 04 Jun 2018 03:49:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 04 Jun 2018 03:49:40: #1 tag size is determined as 50 bps INFO @ Mon, 04 Jun 2018 03:49:40: #1 tag size = 50 INFO @ Mon, 04 Jun 2018 03:49:40: #1 total tags in treatment: 7187420 INFO @ Mon, 04 Jun 2018 03:49:40: #1 user defined the maximum tags... INFO @ Mon, 04 Jun 2018 03:49:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 04 Jun 2018 03:49:40: #1 tags after filtering in treatment: 7187420 INFO @ Mon, 04 Jun 2018 03:49:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 04 Jun 2018 03:49:40: #1 finished! INFO @ Mon, 04 Jun 2018 03:49:40: #2 Build Peak Model... INFO @ Mon, 04 Jun 2018 03:49:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 04 Jun 2018 03:49:40: #1 tags after filtering in treatment: 7187420 INFO @ Mon, 04 Jun 2018 03:49:40: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 04 Jun 2018 03:49:40: #1 finished! INFO @ Mon, 04 Jun 2018 03:49:40: #2 Build Peak Model... INFO @ Mon, 04 Jun 2018 03:49:40: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 04 Jun 2018 03:49:41: #2 number of paired peaks: 0 WARNING @ Mon, 04 Jun 2018 03:49:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 04 Jun 2018 03:49:41: Process for pairing-model is terminated! cat: SRX2766304.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766304.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766304.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766304.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Mon, 04 Jun 2018 03:49:41: #2 number of paired peaks: 0 WARNING @ Mon, 04 Jun 2018 03:49:41: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 04 Jun 2018 03:49:41: Process for pairing-model is terminated! cat: SRX2766304.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX2766304.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766304.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX2766304.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。